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Article

A Study on Myogenesis by Regulation of Reactive Oxygen Species and Cytotoxic Activity by Selenium Nanoparticles

by 1,2,3,†, 1,3,†, 1,4,†, 5, 1, 1,6,7, 1,2,3,6,8,9,10, 1,2,3,6,* and 1,2,3,6,8,9,10,*
1
Institute of Tissue Regeneration Engineering (ITREN), Dankook University, 119 Dandae-ro, Cheonan 31116, Chungcheongnam-do, Korea
2
Department of Biomaterials Science, College of Dentistry, Dankook University, 119 Dandae-ro, Cheonan 31116, Chungcheongnam-do, Korea
3
Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, 119 Dandae-ro, Cheonan 31116, Chungcheongnam-do, Korea
4
Department of Materials Science and Engineering, Korea University, Seoul 02841, Korea
5
Department of Dental Hygiene, Hanseo University, Seosan 31962, Korea
6
UCL Eastman-Korea Dental Medicine Innovation Centre, Dankook University, 119 Dandae-ro, Cheonan 31116, Chungcheongnam-do, Korea
7
Division of Biomaterials and Tissue Engineering, Eastman Dental Institute, University College London, London WC1E 6HH, UK
8
Cell & Matter Institute, Dankook University, Cheonan 31116, Chungcheongnam-do, Korea
9
Department of Regenerative Dental Medicine, College of Dentistry, Dankook University, Cheonan 31116, Chungcheongnam-do, Korea
10
Mechanobiology Dental Medicine Research Center, Cheonan 31116, Chungcheongnam-do, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work as first authors.
Academic Editors: Alessandra Napolitano and Stanley Omaye
Antioxidants 2021, 10(11), 1727; https://doi.org/10.3390/antiox10111727
Received: 14 September 2021 / Revised: 21 October 2021 / Accepted: 27 October 2021 / Published: 29 October 2021
Reactive oxygen species (ROS) are continuously produced by skeletal muscle during contractile activity and even at rest. However, the ROS generated from excessive exercise or traumatic damage may produce more ROS than can be neutralized by an antioxidant capacity, which can be harmful to muscle function. In particular, selenium is a known antioxidant that regulates physiological functions such as cell differentiation and anti-inflammatory function. In this study, we developed nano-sized antioxidative biomaterials using selenium to investigate the protective and differentiation effects against C2C12 myoblasts in an H2O2-induced oxidative stress environment. The selenium nanoparticles (SeNPs) were produced with a size of 35.6 ± 4.3 nm and showed antioxidant effects according to the 3,3′,5,5′-tetramethylbenzidine assay. Then, SeNPs were treated to C2C12 cells with or without H2O2. Our results showed that SeNPs reduced C2C12 apoptosis and intracellular ROS levels. Additionally, SeNPs effectively up-regulated in the presence of H2O2, MyoD, MyoG, α-actinin, and myosin heavy chain, which are well known to increase during myoblast differentiation as assayed by qRT-PCR, immunocytochemistry-staining, western blotting. These results demonstrate that SeNPs can accelerate differentiation with its protective effects from the ROS environment and can be applied to the treatment of skeletal muscle in a cellular redox environment. View Full-Text
Keywords: C2C12; skeletal muscle differentiation; selenium nanoparticle; ROS; antioxidant C2C12; skeletal muscle differentiation; selenium nanoparticle; ROS; antioxidant
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MDPI and ACS Style

Lee, S.-C.; Lee, N.-H.; Patel, K.D.; Jun, S.-K.; Park, J.-H.; Knowles, J.C.; Kim, H.-W.; Lee, H.-H.; Lee, J.-H. A Study on Myogenesis by Regulation of Reactive Oxygen Species and Cytotoxic Activity by Selenium Nanoparticles. Antioxidants 2021, 10, 1727. https://doi.org/10.3390/antiox10111727

AMA Style

Lee S-C, Lee N-H, Patel KD, Jun S-K, Park J-H, Knowles JC, Kim H-W, Lee H-H, Lee J-H. A Study on Myogenesis by Regulation of Reactive Oxygen Species and Cytotoxic Activity by Selenium Nanoparticles. Antioxidants. 2021; 10(11):1727. https://doi.org/10.3390/antiox10111727

Chicago/Turabian Style

Lee, Sang-Cheol, Na-Hyun Lee, Kapil D. Patel, Soo-Kyung Jun, Jeong-Hui Park, Jonathan C. Knowles, Hae-Won Kim, Hae-Hyoung Lee, and Jung-Hwan Lee. 2021. "A Study on Myogenesis by Regulation of Reactive Oxygen Species and Cytotoxic Activity by Selenium Nanoparticles" Antioxidants 10, no. 11: 1727. https://doi.org/10.3390/antiox10111727

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