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Open AccessFeature PaperArticle

A Facile One-Pot Synthesis of Water-Soluble, Patchy Fe3O4-Au Nanoparticles for Application in Radiation Therapy

1
Department Chemistry and Pharmacy, Physical Chemistry I and ICMM, Friedrich-Alexander University of Erlangen, Egerlandstr. 3, D-91058 Erlangen, Germany
2
French-German Research Institute of Saint-Louis, 5 rue du Général Cassagnou, F-68300 Saint-Louis, France
3
Department of Radiation Oncology, Friedrich-Alexander University of Erlangen, Universitätsstr. 27, D-91054 Erlangen, Germany
4
Department of Anatomy, Chair of Anatomy I, Friedrich-Alexander University of Erlangen, Krankenhausstr. 9, D-91054 Erlangen, Germany
*
Author to whom correspondence should be addressed.
Appl. Sci. 2019, 9(1), 15; https://doi.org/10.3390/app9010015
Received: 15 November 2018 / Revised: 14 December 2018 / Accepted: 18 December 2018 / Published: 21 December 2018
(This article belongs to the Special Issue Magnetic Nanomaterials for Drug Delivery and Therapy)
A facile one-pot synthesis route for the preparation of water-soluble, biocompatible patchy Fe3O4-Au nanoparticles (Fe3O4-Au pNPs) was developed. Biocompatibility was attained through surface functionalization with 1-methyl-3-(dodecylphosphonic acid) imidazolium bromide. The morphology, composition, crystal structure and magnetic properties of the Fe3O4-Au pNPs were investigated by conducting experiments with transmission electron microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction and superconducting quantum interference device, respectively. Internalization of the Fe3O4-Au pNPs by MCF-7 cells occurred via endocytosis. The performance of the Fe3O4-Au pNPs as X-ray radiosensitizer in tumor cells was compared with that of gold nanocluster and Fe3O4 NPs. For this reason, MCF-7, A549 and MCF-10A cells were loaded with the respective kind of nanoparticles and treated with X-rays at doses of 1, 2 or 3 Gy. The nanoparticle-induced changes of the concentration of the reactive oxygen species (ROS) were detected using specific assays, and the cell survival under X-ray exposure was assessed employing the clonogenic assay. In comparison with the gold nanocluster and Fe3O4 NPs, the Fe3O4-Au pNPs exhibited the highest catalytic capacity for ROS generation in MCF-7 and A549 cells, whereas in the X-ray-induced ROS formation in healthy MCF-10A cells was hardly enhanced by the Fe3O4 NPs and Fe3O4-Au pNPs. Moreover, the excellent performance of Fe3O4-Au pNPs as X-ray radiosensitizers was verified by the quickly decaying radiation dose survival curve of the nanoparticle-loaded MCF-7 and A549 cells and corroborated by the small values of the associated dose-modifying factors. View Full-Text
Keywords: X-ray radiosensitizer; radiotherapy; patchy Fe3O4-Au nanoparticles; MCF-7 cells; A549 cells; MCF-10A cells X-ray radiosensitizer; radiotherapy; patchy Fe3O4-Au nanoparticles; MCF-7 cells; A549 cells; MCF-10A cells
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MDPI and ACS Style

Klein, S.; Hübner, J.; Menter, C.; Distel, L.V.R.; Neuhuber, W.; Kryschi, C. A Facile One-Pot Synthesis of Water-Soluble, Patchy Fe3O4-Au Nanoparticles for Application in Radiation Therapy. Appl. Sci. 2019, 9, 15.

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