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Appl. Sci. 2018, 8(12), 2517; https://doi.org/10.3390/app8122517

Valorization of Waste Glycerol to Dihydroxyacetone with Biocatalysts Obtained from Gluconobacter oxydans

1
Department of Biotechnology, Microbiology and Food Evaluation, Faculty of Food Sciences, Warsaw University of Life Sciences-SGGW, Nowoursynowska St. 166, 02-787 Warsaw, Poland
2
The Energy and Resources Institute (TERI), Darbari Seth Block, IHC Complex, Lodhi Road, New Delhi 110003, India
*
Author to whom correspondence should be addressed.
Received: 20 October 2018 / Revised: 10 November 2018 / Accepted: 29 November 2018 / Published: 6 December 2018
(This article belongs to the Special Issue Hazardous Waste Treatment)
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Abstract

Waste glycerol is the main by-product generated during biodiesel production, in an amount reaching up to 10% of the produced biofuel. Is there any method which allows changing this waste into industrial valuable compounds? This manuscript describes a method for valorization of crude glycerol via microbial bioconversion. It has been shown that the use of free and immobilized biocatalysts obtained from Gluconobacter oxydans can enable beneficial valorization of crude glycerol to industrially valuable dihydroxyacetone. The highest concentration of this compound, reaching over 20 g·L−1, was obtained after 72 h of biotransformation with free G. oxydans cells, in a medium containing 30 or 50 g·L−1 of waste glycerol. Using a free cell extract resulted in higher concentrations of dihydroxyacetone and a higher valorization efficiency (up to 98%) compared to the reaction with an immobilized cell extract. Increasing waste glycerol concentration to 50 g·L−1 causes neither a faster nor higher increase in product yield and reaction efficiency compared to its initial concentration of 30 g·L−1. The proposed method could be an alternative for utilization of a petrochemical waste into industry applicated chemicals. View Full-Text
Keywords: waste/crude glycerol; dihydroxyacetone; cell extract; immobilization; Gluconobacter oxydans; glycerol dehydrogenase waste/crude glycerol; dihydroxyacetone; cell extract; immobilization; Gluconobacter oxydans; glycerol dehydrogenase
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Stasiak-Różańska, L.; Berthold-Pluta, A.; Dikshit, P.K. Valorization of Waste Glycerol to Dihydroxyacetone with Biocatalysts Obtained from Gluconobacter oxydans. Appl. Sci. 2018, 8, 2517.

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