Next Article in Journal
Nonlinear Dynamic Response of Ropeway Roller Batteries via an Asymptotic Approach
Previous Article in Journal
Energy Transformation on Vault in Elite Artistic Gymnastics: Comparisons between Simple and Difficult Tsukahara and Yurchenko Vaults
Article

Decellularized Skeletal Muscles Support the Generation of In Vitro Neuromuscular Tissue Models

1
Istituto di Ricerca Pediatrica “Città della Speranza”, 35127 Padova, Italy
2
Department of Molecular Medicine, University of Padova, 35121 Padova, Italy
3
UCL Great Ormond Street Institute of Child Health, London WC1N 1EH, UK
4
UCL Royal Free Hospital—Division of Surgery and Interventional Sciences, London NW3 2PS, UK
5
Department of Biomedical Sciences, University of Padova, 35131 Padova, Italy
6
Veneto Institute Molecular Medicine (VIMM) Fondazione Ricerca Biomedica Avanzata ONLUS, 35129 Padova, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Barbara Nebe
Appl. Sci. 2021, 11(20), 9485; https://doi.org/10.3390/app11209485
Received: 17 September 2021 / Revised: 5 October 2021 / Accepted: 7 October 2021 / Published: 13 October 2021
Decellularized skeletal muscle (dSkM) constructs have received much attention in recent years due to the versatility of their applications in vitro. In search of adequate in vitro models of the skeletal muscle tissue, the dSkM offers great advantages in terms of the preservation of native-tissue complexity, including three-dimensional organization, the presence of residual signaling molecules within the construct, and their myogenic and neurotrophic abilities. Here, we attempted to develop a 3D model of neuromuscular tissue. To do so, we repopulated rat dSkM with human primary myogenic cells along with murine fibroblasts and we coupled them with organotypic rat spinal cord samples. Such culture conditions not only maintained multiple cell type viability in a long-term experimental setup, but also resulted in functionally active construct capable of contraction. In addition, we have developed a customized culture system which enabled easy access, imaging, and analysis of in vitro engineered co-cultures. This work demonstrates the ability of dSkM to support the development of a contractile 3D in vitro model of neuromuscular tissue fit for long-term experimental evaluations. View Full-Text
Keywords: decellularized muscle; co-culture; neuromuscular model; 3D culture; skeletal muscle; spinal cord culture decellularized muscle; co-culture; neuromuscular model; 3D culture; skeletal muscle; spinal cord culture
Show Figures

Figure 1

MDPI and ACS Style

Raffa, P.; Easler, M.; Cecchinato, F.; Auletta, B.; Scattolini, V.; Perin, S.; Gerli, M.F.M.; Caccin, P.; Elvassore, N.; De Coppi, P.; Urciuolo, A. Decellularized Skeletal Muscles Support the Generation of In Vitro Neuromuscular Tissue Models. Appl. Sci. 2021, 11, 9485. https://doi.org/10.3390/app11209485

AMA Style

Raffa P, Easler M, Cecchinato F, Auletta B, Scattolini V, Perin S, Gerli MFM, Caccin P, Elvassore N, De Coppi P, Urciuolo A. Decellularized Skeletal Muscles Support the Generation of In Vitro Neuromuscular Tissue Models. Applied Sciences. 2021; 11(20):9485. https://doi.org/10.3390/app11209485

Chicago/Turabian Style

Raffa, Paolo, Maria Easler, Francesca Cecchinato, Beatrice Auletta, Valentina Scattolini, Silvia Perin, Mattia F.M. Gerli, Paola Caccin, Nicola Elvassore, Paolo De Coppi, and Anna Urciuolo. 2021. "Decellularized Skeletal Muscles Support the Generation of In Vitro Neuromuscular Tissue Models" Applied Sciences 11, no. 20: 9485. https://doi.org/10.3390/app11209485

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop