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Article

Flow Control Techniques for Enhancing the Bio-Recognition Performance of Microfluidic-Integrated Biosensors

1
Department of Mechanical, Aerospace and Civil Engineering, University of Manchester, Manchester M13 9PL, UK
2
Harvard John A. Paulson School of Engineering and Applied Sciences, Harvard University, Allston, MA 02134, USA
3
Manchester Academic Health Science Centre, Manchester University NHS Foundation Trust, Southmoor Road, Wythenshawe, Manchester M13 9PL, UK
*
Author to whom correspondence should be addressed.
Academic Editors: Jianzhong Lin and Raed Abu-Reziq
Appl. Sci. 2021, 11(15), 7168; https://doi.org/10.3390/app11157168
Received: 1 June 2021 / Revised: 8 July 2021 / Accepted: 26 July 2021 / Published: 3 August 2021
(This article belongs to the Special Issue Fluid Flows Modelling in Microfluidic Systems)
Biosensors are favored devices for the fast and cost-effective detection of biological species without the need for laboratories. Microfluidic integration with biosensors has advanced their capabilities in selectivity, sensitivity, controllability, and conducting multiple binding assays simultaneously. Despite all the improvements, their design and fabrication are still challenging and time-consuming. The current study aims to enhance microfluidic-integrated biosensors’ performance. Three different functional designs are presented with both active (with the help of electroosmotic flow) and passive (geometry optimization) methods. For validation and further studies, these solutions are applied to an experimental setup for DNA hybridization. The numerical results for the original case have been validated with the experimental data from previous literature. Convection, diffusion, migration, and hybridization of DNA strands during the hybridization process have been simulated with finite element method (FEM) in 3D. Based on the results, increasing the velocity on top of the functionalized surface, by reducing the thickness of the microchamber in that area, would increase the speed of surface coverage by up to 62%. An active flow control with the help of electric field would increase this speed by 32%. In addition, other essential parameters in the fabrication of the microchamber, such as changes in pressure and bulk concentration, have been studied. The suggested designs are simple, applicable and cost-effective, and would not add extra challenges to the fabrication process. Overall, the effect of the geometry of the microchamber on the time and effectiveness of biosensors is inevitable. More studies on the geometry optimization of the microchamber and position of the electrodes using machine learning methods would be beneficial in future works. View Full-Text
Keywords: engineering; computational fluid dynamics; mass transfer in microfluidic systems; biosensor design and optimization; electroosmotic flow engineering; computational fluid dynamics; mass transfer in microfluidic systems; biosensor design and optimization; electroosmotic flow
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MDPI and ACS Style

Shahbazi, F.; Souri, M.; Jabbari, M.; Keshmiri, A. Flow Control Techniques for Enhancing the Bio-Recognition Performance of Microfluidic-Integrated Biosensors. Appl. Sci. 2021, 11, 7168. https://doi.org/10.3390/app11157168

AMA Style

Shahbazi F, Souri M, Jabbari M, Keshmiri A. Flow Control Techniques for Enhancing the Bio-Recognition Performance of Microfluidic-Integrated Biosensors. Applied Sciences. 2021; 11(15):7168. https://doi.org/10.3390/app11157168

Chicago/Turabian Style

Shahbazi, Fatemeh, Mohammad Souri, Masoud Jabbari, and Amir Keshmiri. 2021. "Flow Control Techniques for Enhancing the Bio-Recognition Performance of Microfluidic-Integrated Biosensors" Applied Sciences 11, no. 15: 7168. https://doi.org/10.3390/app11157168

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