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HMT Exerts an Anticancer Effect by Targeting PAK-1

1
Department of Science in Korean Medicine, College of Korean Medicine, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul 02435, Korea
2
Department of Food Science & Services, Eulji University, Seongnam 13135, Kyungido, Korea
3
Department of Cancer Preventive Material Development, College of Korean Medicine, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul 02435, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Domenico Osella
Appl. Sci. 2021, 11(13), 6034; https://doi.org/10.3390/app11136034
Received: 31 May 2021 / Revised: 25 June 2021 / Accepted: 27 June 2021 / Published: 29 June 2021
Heamatang (HMT) is a classic medicinal formula used in traditional Chinese and Korean medicine; it contains seven distinct components, mainly of herbal origin. HMT is used as an antiaging remedy, treating urinary disorders and increasing energy and vitality. However, the therapeutic applications of this formula have not been evaluated with evidence-based science. Therefore, we assessed HMT through various in vitro methods, including cell viability assay, fluorescence-activated cell sorting assay (FACS), Western blotting, migration assay, three-dimensional (3D) cell culture, siRNA-mediated PAK-1 knockdown, and crystal violet assays. HMT decreased PAK-1 expression in PC-3 cells and inhibited cell viability, growth, and motility. The inhibition of cell motility by HMT was correlated with PAK-1-mediated inhibition of Lim domain kinase (LIMK) and cofilin. HMT induced G1 arrest and apoptosis through the transcriptional regulation of cell cycle regulatory proteins and apoptosis-related proteins (increase in c-cas3 and inhibition of PARP and BCL-2). Moreover, HMT suppressed PAK-1 expression, leading to the inhibition of AKT activities. Finally, we showed that decursin was the active ingredient involved in the inhibitory effect of HMT on PAK-1. Our findings demonstrated that HMT exerts its anticancer influence through the inhibition of PAK-1. The HMT formula could be applied in various fields, including functional health food and pharmaceutical development. View Full-Text
Keywords: PAK-1; prostate cancer; complementary and alternative medicine; heamatang; decursin PAK-1; prostate cancer; complementary and alternative medicine; heamatang; decursin
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MDPI and ACS Style

Xu, Y.; Cha, J.-S.; Lee, S.-O.; Ryu, S.-I.; Lee, Y.-K.; Han, H.; Kim, J.-E.; Lee, M.-H.; Lee, E.-O.; Lee, H.-J. HMT Exerts an Anticancer Effect by Targeting PAK-1. Appl. Sci. 2021, 11, 6034. https://doi.org/10.3390/app11136034

AMA Style

Xu Y, Cha J-S, Lee S-O, Ryu S-I, Lee Y-K, Han H, Kim J-E, Lee M-H, Lee E-O, Lee H-J. HMT Exerts an Anticancer Effect by Targeting PAK-1. Applied Sciences. 2021; 11(13):6034. https://doi.org/10.3390/app11136034

Chicago/Turabian Style

Xu, Yinzhu, Jin-Sol Cha, Seon-Ok Lee, Soo-In Ryu, You-Kyung Lee, Hengmin Han, Jung-Eun Kim, Min-Ho Lee, Eun-Ok Lee, and Hyo-Jeong Lee. 2021. "HMT Exerts an Anticancer Effect by Targeting PAK-1" Applied Sciences 11, no. 13: 6034. https://doi.org/10.3390/app11136034

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