Effect of Different Synchronization Regimens on Reproductive Variables of Crossbred (Swamp × Riverine) Nulliparous and Multiparous Buffaloes during Peak and Low Breeding Seasons
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animal Ethics
2.2. Description of Experimental Animals and Husbandry Practices
2.3. Estrus Synchronization Protocols
2.4. Follicular Dynamics, Estrus Detection, AI, and Pregnancy Diagnosis
2.5. Statistical Analyses
3. Results
3.1. Effect of Parity, Protocol, and Breeding Season on the Reproductive Performance in Crossbred Buffaloes
3.2. Effect of Synchronization Protocols on the Reproductive Parameters of Different Parities of Buffaloes
3.3. Effect of Protocols on the Reproductive Performance of Buffaloes in Different Seasons
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- Singh, J.; Nanda, A.S.; Adams, G.P. The reproductive pattern and efficiency of female buffaloes. Anim. Reprod. Sci. 2000, 61, 593–604. [Google Scholar] [CrossRef]
- Barile, V.L.; Terzano, G.M.; Pacelli, C.; Todini, L.; Malfatti, A.; Barbato, O. LH peak and ovulation after two different estrus synchronization treatments in buffalo cows in the daylight-lengthening period. Theriogenology 2015, 84, 286–293. [Google Scholar] [CrossRef]
- Giuseppe, C.; Gianluca, N.; Bianca, G.; Giorgio, G.; Alberto, P.; Rossella, D.P.; D’Occhio, M.J.; Luigi, Z. Embryonic mortality in buffaloes synchronized and mated by AI during the seasonal decline in reproductive function. Theriogenology 2005, 63, 2334–2340. [Google Scholar]
- Rossi, P.; Vecchio, D.; Neglia, G.; Di, P.R.; Gasparrini, B.; D’Occhio, M.J.; Campanile, G. Seasonal fluctuations in the response of Italian Mediterranean buffaloes to synchronization of ovulation and timed artificial insemination. Theriogenology 2014, 82, 132–137. [Google Scholar] [CrossRef]
- Presicce, G.A.; Senatore, E.M.; Bella, A.; Santis, G.D.; Barile, V.L.; Mauro, G.J.D.; Terzano, G.M.; Stecco, R.; Parmeggiani, A. Ovarian follicular dynamics and hormonal profiles in heifer and mixed-parity Mediterranean Italian buffaloes (Bubalus bubalis) following an estrus synchronization protocol. Theriogenology 2004, 61, 1343–1355. [Google Scholar] [CrossRef] [PubMed]
- Baruselli, P.S.; Soares, J.G.; Bayeux, B.M.; Silva, J.C.B.; Mingoti, R.D.; Carvalho, N.A.T. Assisted reproductive technologies (ART) in water buffaloes. Anim. Reprod. 2018, 15, 971–983. [Google Scholar] [CrossRef]
- Campanile, G.; Baruselli, P.S.; Neglia, G.; Vecchio, D.; Gasparrini, B.; Gimenes, L.U.; Zicarelli, L.; D’Occhio, M.J. Ovarian function in the buffalo and implications for embryo development and assisted reproduction. Anim. Reprod. Sci. 2010, 12, 1–11. [Google Scholar] [CrossRef]
- Campanile, G.; Vecchio, D.; Di, P.R.; Neglia, G.; Gasparrini, B.; Prandi, A.; Zicarelli, L.; D’Occhio, M.J. Delayed treatment with GnRH agonist, hCG and progesterone and reduced embryonic mortality in buffaloes. Theriogenology 2008, 70, 1544–1549. [Google Scholar] [CrossRef]
- Carvalho, N.; Soares, J.; Reis, E.; Baruselli, P. Use of DIB and CRESTAR to synchronize ovulation and FTAI in buffalo heifers during the non-breeding season. Acta Sci. Vet. 2011, 16, 390–394. [Google Scholar]
- Barile, V. Technologies Related with the Artificial Insemination in Buffalo. J. Buffalo Sci. 2012, 1, 139–146. [Google Scholar] [CrossRef]
- De Rensis, F.; Ronci, G.; Guarneri, P.; Nguyen, B.X.; Presicce, G.A.; Huszenicza, G.; Scaramuzzi, R.J. Conception rate after fixed time insemination following ovsynch protocol with and without progesterone supplementation in cyclic and non-cyclic Mediterranean Italian buffaloes (Bubalus bubalis). Theriogenology 2005, 63, 1824–1831. [Google Scholar] [CrossRef]
- Du, C.; Nan, L.; Sabek, A.; Wang, H.; Luo, X.; Hua, G.; Zhang, S. Evaluation of Ovsynch versus modified Ovsynch program on pregnancy rate in water buffaloes: A meta-analysis. Trop. Anim. Health Prod. 2021, 53, 397. [Google Scholar] [CrossRef]
- Warriach, H.M.; Ahmad, N. Follicular waves during the oestrous cycle in Nili-Ravi buffaloes undergoing spontaneous and PGF2alpha-induced luteolysis. Anim. Reprod. Sci. 2007, 101, 332–337. [Google Scholar] [CrossRef]
- Carvalho, N.A.T.; Nichi, M.; Henriquez, C.E.P.; Oliveira, C.A.; Baruselli, P.S. Use of Human Chorionic Gonadotropin (hCG) for fixed-time artificial insemination in buffalo (Bubalus bubalis). Anim. Reprod. 2007, 4, 98–102. [Google Scholar]
- Berber, R.C.D.A.; Madureira, E.H.; Baruselli, P.S. Comparison of two Ovsynch protocols (GnRH versus LH) for fixed timed insemination in buffalo (Bubalus bubalis). Theriogenology 2002, 57, 1421–1430. [Google Scholar] [CrossRef]
- Karuppanasamy, K.; Sharma, R.K.; Phulia, S.K.; Jerome, A.; Kavya, K.M.; Ghuman, S.P.S.; Kumar, H.; Singh, I.; Krishnaswamy, N. Ovulation and fertility response using modified Heatsynch and Ovsynch protocols in the anovular Murrah buffalo (Bubalus bubalis). Theriogenology 2017, 95, 83–86. [Google Scholar] [CrossRef]
- Rathore, R.; Sharma, R.K.; Phulia, S.K.; Mudgal, V.; Jerome, A.; Ghuman, S.P.S.; Singh, I. Comparative efficacy of oestrus synchronization protocols in buffalo (Bubalus bubalis). Trop. Anim. Health Prod. 2017, 49, 1–6. [Google Scholar] [CrossRef] [PubMed]
- Armando José, O.; Rojas, A.F.; Velazquez, M.A.; Muro, J.D.; Márquez, Y.C.; Vilanova, L.T. Efficiency of two timed artificial insemination protocols in Murrah buffaloes managed under a semi-intensive system in the tropics. Trop. Anim. Health Prod. 2010, 42, 1149–1154. [Google Scholar]
- Waqas, M.; Mehmood, M.; Shahzad, Q.; Kausar, R.; Sattar, A.; Naseer, Z. Comparative efficacy of G6G and Ovsynch protocols on synchronization and pregnancy rate in Nili-Ravi buffalo. Anim. Reprod. Sci. 2016, 166, 9–14. [Google Scholar] [CrossRef]
- Kalwar, Q.; Ahmedmemon, A.; Bhutto, M.B.; Kunbhar, H.K.; Mirani, A.H.; Anwar, M.; Ahmedwagan, S. Estrus response and fertility rate in Kundhi buffaloes following estrus synchronization in breeding season. J. Adv. Res. 2015, 14, 362–365. [Google Scholar] [CrossRef]
- Gupta, K.K.; Shukla, S.N.; Inwati, P.; Shrivastava, O.P. Fertility response in postpartum anoestrus buffaloes (Bubalus bubalis) using modified Ovsynch based timed insemination protocols. Vet. World 2015, 8, 316–319. [Google Scholar] [CrossRef]
- Bahareldin-Ali, A.; Qin, G.; Guo, R.; Tsigkou, A.; Tan, Z.; Huang, J.; Li, H.; Li, H.; Shi, Z. Endocrine and ovarian responses in water buffalo cows immunized against inhibin and subjected to the Ovsynch protocol. J. Integrat. Agric. 2015, 14, 1827–1837. [Google Scholar] [CrossRef]
- Mirmahmoudi, R.; Prakash, B.S. The endocrine changes, the timing of ovulation and the efficacy of the Doublesynch protocol in the Murrah buffalo (Bubalus bubalis). Gen. Comp. Endocrinol. 2012, 177, 153–159. [Google Scholar] [CrossRef] [PubMed]
- Abulaiti, A.; El-Qaliouby, H.S.; El Bahgy, H.E.K.; Naseer, Z.; Ahmed, Z.; Hua, G.; Yang, L. GPGMH, a New Fixed Timed-AI Synchronization Regimen for Swamp and River Crossbred Buffaloes (Bubalus bubalis). Front. Vet. Sci. 2021, 8, 646247. [Google Scholar] [CrossRef]
- Carvalho, N.A.; Soares, J.G.; Souza, D.C.; Vannucci, F.S.; Amaral, R.; Maio, J.R.; Sales, J.N.; Sá Filho, M.F.; Baruselli, P.S. Different circulating progesterone concentrations during synchronization of ovulation protocol did not affect ovarian follicular and pregnancy responses in seasonal anestrous buffalo cows. Theriogenology 2014, 81, 490–495. [Google Scholar] [CrossRef] [PubMed]
- Neglia, G.; Gasparrini, B.; Palo, R.; Rosa, C.; Zicarelli, L.; Campanile, G. Comparison of pregnancy rates with two estrus synchronization protocols in Italian Mediterranean buffalo cows. Theriogenology 2003, 60, 125–133. [Google Scholar] [CrossRef]
- Singh, I.; Balhara, A.K. New approaches in buffalo artificial insemination programs with special reference to India. Theriogenology 2016, 86, 194–199. [Google Scholar] [CrossRef] [PubMed]
- Campanile, G.; Di Palo, R.; Neglia, G.; Vecchio, D.; Gasparrini, B.; Prandi, A.; Galiero, G.; D’Occhio, M.J. Corpus luteum function and embryonic mortality in buffaloes treated with a GnRH agonist, hCG and progesterone. Theriogenology 2007, 67, 1393–1398. [Google Scholar] [CrossRef] [PubMed]
- Sharma, R.K.; Phulia, S.K.; Jerome, A.; Singh, I. Ovsynch Plus protocol improves ovarian response in anovular Murrah buffaloes in low-breeding season. Reprod. Dom. Anim. 2017, 52, 1030–1035. [Google Scholar] [CrossRef]
- Yendraliza Handoko, J.; Rodiallah, M. Reproductive performance of buffalo-cows with various synchronization protocols in kampar regency of Riau province. IOP Conf. Ser. Earth Environ. Sci. 2019, 260, 12057. [Google Scholar] [CrossRef]
- Brito, L.F.C.; Satrap, R.; Marson, E.P.; Kastelic, J.P. Efficacy of PGF2α to synchronize estrus in water buffalo cows (Bubalus bubalis) is dependent upon plasma progesterone concentration, corpus luteum size and ovarian follicular status. Anim. Reprod. Sci. 2002, 73, 23–35. [Google Scholar] [CrossRef]
- Derar, R.; Hussein, H.A.; Fahmy, S.; El-Sherry, T.M.; Megahed, G. The effect of parity on the efficacy of an ovulation synchronization (Ovsynch) protocol in buffalo (Bubalus bubalis). Anim. Reprod. 2012, 9, 52–60. [Google Scholar]
- Carvalho, N.A.; Soares, J.G.; Souza, D.C.; Maio, J.R.; Sales, J.N.; Martins, J.B.; Macari, R.C.; D’Occhio, M.J.; Baruselli, P.S. Ovulation synchronization with estradiol benzoate or GnRH in a timed artificial insemination protocol in buffalo cows and heifers during the nonbreeding season. Theriogenology 2017, 87, 333–338. [Google Scholar] [CrossRef] [PubMed]
Variables | Protocol | Parity | Season | Main Effects | Interactions | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
GPGMH (n = 216) | OVS (n = 66) | Multiparous (n = 154) | Nulliparous (n = 128) | PBS (n = 186) | LBS (n = 96) | Protocol | Parity | Season | Protocol × Parity | Protocol × Season | Parity × Season | Protocol × Parity × Season | |
Follicle diameter at day 6 (mm) | 7.3 ± 2.3 | 8.2 ± 2.6 | 8.6 ± 2.6 | 6.9 ± 1.9 | 7.4 ± 0.9 | 7.5 ± 0.9 | 0.020 | <0.0001 | 0.5705 | 0.290 | 0.327 | 0.617 | 0.249 |
Interval to estrus after 2nd GnRH (h) | 9.7 ± 1.1 | 8.5 ± 1.2 | 9.1 ± 1.1 | 9.2 ± 1.2 | 8.9 ± 0.7 | 9.3 ± 0.7 | <0.0001 | 0.722 | 0.0500 | 0.043 | 0.028 | 0.402 | 0.548 |
Estrus response (%) | 186/216 (86.1) | 50/66 (75.8) | 139/154 (89.0) | 97/128 (75.8) | 162/186 (87.1) | 74/96 (77.1) | 0.0463 | 0.022 | 0.0105 | 0.042 | 0.014 | 0.156 | 0.134 |
Estrus duration (h) | 15.7 ± 1.3 | 14.1 ± 1.7 | 16.0 ± 0.9 | 14.9 ± 1.6 | 15.0 ± 0.6 | 15.1 ± 0.5 | 0.036 | <0.0001 | 0.7070 | 0.716 | 0.822 | 0.461 | 0.252 |
Silent estrus rate (%) | 35/216 (16.2) | 19/66 (28.8) | 26/154 (16.9) | 28/128 (21.9) | 28/186 (15.1) | 26/96 (27.1) | 0.0017 | 0.084 | 0.2780 | 0.024 | 0.031 | 0.625 | 0.169 |
Interval to ovulation after 2nd GnRH (h) | 24.4 ± 0.9 | 23.8 ± 0.9 | 24.4 ± 0.9 | 23.7 ± 0.9 | 24.3 ± 0.5 | 23.9 ± 0.4 | 0.0002 | <0.0001 | 0.0106 | 0.047 | 0.765 | 0.075 | 0.164 |
Ovulation follicle diameter (mm) | 13.1 ± 2.3 | 13.0 ± 1.8 | 13.9 ± 1.8 | 12.1 ± 1.7 | 13.7 ± 0.8 | 13.3 ± 0.8 | 0.898 | <0.0001 | 0.0938 | 0.051 | 0.879 | 0.467 | 0.392 |
Ovulation rate (%) | 177/216 (81.9) | 43/66 (65.2) | 130/154 (84.4) | 90/128 (70.3) | 154/186 (82.7) | 66/96 (68.8) | 0.0377 | 0.0044 | 0.0012 | 0.053 | 0.013 | 0.022 | 0.149 |
Incidence of follicular cysts (%) | 5/216 (2.3) | 2/66 (3.0) | 4/154 (2.6) | 3/128 (2.3) | 4/186 (2.2) | 3/96 (3.1) | 0.874 | 0.736 | 0.2890 | 0.713 | 0.790 | 0.449 | 0.359 |
Pregnancy rate (%) | 98/216 (45.4) | 21/66 (31.8) | 73/154 (47.4) | 46/128 (35.9) | 85/186 (45.7) | 34/96 (35.4) | 0.0511 | 0.0137 | 0.0372 | 0.300 | 0.0530 | 0.519 | 0.123 |
Variables | Multiparous (n = 154) | Nulliparous (n = 128) | Statistical Effect and Interaction * | ||||
---|---|---|---|---|---|---|---|
GPGMH (n = 122) | OVS (n = 32) | GPGMH (n = 94) | OVS (n = 34) | p | PT | p × PT | |
Follicle diameter at day 6 (mm) | 8.4 ± 2.3 | 8.7 ± 2.8 | 6.1 ± 1.6 B | 7.6 ± 2.2 A | 0.0003 | 0.0514 | 0.1774 |
Interval to estrus onset after 2nd GnRH (h) | 9.9 ± 1.2 a | 8.3 ± 1.0 b | 9.5 ± 0.9 | 8.8 ± 1.4 | 0.7888 | 0.0001 | 0.0424 |
Estrus response (%) | 114/122 (93.4) a | 25/32 (78.1) b | 72/94 (76.6) | 25/34 (73.5) | 0.0030 | 0.1410 | 0.0210 |
Estrus duration (h) | 16.2 ± 1.2 | 15.7 ± 0.7 | 15.2 ± 1.1 | 14.5 ± 2.1 | <0.0001 | 0.0350 | 0.6305 |
Silent estrus rate (%) | 15/122 (12.3) b | 11/32 (34.4) a | 20/94 (21.3) | 8/34 (23.5) | 0.0080 | 0.0840 | 0.0100 |
Interval to ovulation after 2nd GnRH (h) | 24.6 ± 0.9 | 24.3 ± 0.8 | 24.2 ± 0.9 | 23.2 ± 0.8 | <0.0001 | 0.0027 | 0.0310 |
Ovulation follicle diameter (mm) | 14.4 ± 2.2 | 13.5 ± 1.4 | 11.8 ± 1.5 | 12.5 ± 2.0 | <0.0001 | 0.7839 | 0.0200 |
Ovulation rate (%) | 109/122 (89.3) a | 21/32 (65.6) b | 68/94 (72.3) | 22/34 (64.7) | 0.0056 | 0.1060 | 0.0470 |
Incidence of follicular cysts (%) | 3/122 (2.5) | 1/32 (3.1) | 2/94 (2.1) | 1/34 (2.9) | 0.7347 | 0.9018 | 0.9600 |
Pregnancy rate (%) | 62/122 (50.8) a | 11/32 (34.4) b | 36/94 (38.3) | 10/34 (29.4) | 0.0170 | 0.0538 | 0.3330 |
Variables | PBS (n = 186) | LBS (n = 96) | Statistical Effect and Interaction * | ||||
---|---|---|---|---|---|---|---|
GPGMH (n = 143) | OVS (n = 43) | GPGMH (n = 73) | OVS (n = 23) | S | PT | S × PT | |
Follicle diameter at day 6 (mm) | 7.2 ± 1.3 | 7.5 ± 1.4 | 7.1 ± 1.2 | 7.9 ± 1.5 | 0.1009 | 0.5580 | 0.4664 |
Interval to estrus onset after 2nd GnRH (h) | 9.2 ± 0.7 | 8.6 ± 1.0 | 9.1 ± 0.7 | 9.5 ± 1.2 | 0.6152 | 0.0410 | 0.0239 |
Estrus response (%) | 129/143 (90.2) a | 33/43 (76.7) b | 57/73 (78.1) | 17/23 (73.9) | 0.0020 | 0.0069 | 0.0940 |
Estrus duration (h) | 15.4 ± 0.7 a | 14.7 ± 0.9 b | 15.5 ± 0.9 A | 14.7 ± 0.8 B | <0.0001 | 0.5398 | 0.9023 |
Silent estrus rate (%) | 16/143 (11.2) b | 12/43 (27.9) a | 19/73 (26.0) | 7/23 (30.4) | 0.0795 | 0.6838 | 0.0009 |
Interval to ovulation after 2nd GnRH (h) | 24.7 ± 0.7a | 23.9 ± 0.6 b | 24.5 ± 0.7 A | 23.4 ± 0.5 B | <0.0001 | 0.0067 | 0.3589 |
Ovulation follicle diameter (mm) | 14.1 ± 1.1 a | 13.6 ± 1.3 b | 14.4 ± 1.0 A | 13.4 ± 1.1 B | 0.4645 | 0.0043 | 0.9656 |
Ovulation rate (%) | 125/143 (87.4) a | 29/43 (67.4) b | 52/73 (71.2) | 14/23 (60.9) | <0.0001 | 0.0003 | 0.0388 |
Incidence of follicular cysts (%) | 3/143 (4.2) | 1//43 (4.7) | 02/73 (6.8) | 1/23 (13.0) | 0.1878 | 0.6794 | 0.3799 |
Pregnancy rate (%) | 70/143 (49.0) a | 15/43 (34.9) b | 28/73 (38.4) | 6/23 (26.1) | 0.0549 | <0.0001 | 0.0019 |
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. |
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
Abulaiti, A.; Naseer, Z.; Ahmed, Z.; Wang, D.; Hua, G.; Yang, L. Effect of Different Synchronization Regimens on Reproductive Variables of Crossbred (Swamp × Riverine) Nulliparous and Multiparous Buffaloes during Peak and Low Breeding Seasons. Animals 2022, 12, 415. https://doi.org/10.3390/ani12040415
Abulaiti A, Naseer Z, Ahmed Z, Wang D, Hua G, Yang L. Effect of Different Synchronization Regimens on Reproductive Variables of Crossbred (Swamp × Riverine) Nulliparous and Multiparous Buffaloes during Peak and Low Breeding Seasons. Animals. 2022; 12(4):415. https://doi.org/10.3390/ani12040415
Chicago/Turabian StyleAbulaiti, Adili, Zahid Naseer, Zulfiqar Ahmed, Dong Wang, Guohua Hua, and Liguo Yang. 2022. "Effect of Different Synchronization Regimens on Reproductive Variables of Crossbred (Swamp × Riverine) Nulliparous and Multiparous Buffaloes during Peak and Low Breeding Seasons" Animals 12, no. 4: 415. https://doi.org/10.3390/ani12040415
APA StyleAbulaiti, A., Naseer, Z., Ahmed, Z., Wang, D., Hua, G., & Yang, L. (2022). Effect of Different Synchronization Regimens on Reproductive Variables of Crossbred (Swamp × Riverine) Nulliparous and Multiparous Buffaloes during Peak and Low Breeding Seasons. Animals, 12(4), 415. https://doi.org/10.3390/ani12040415