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Article

Prevalence and Molecular Characterization of Salmonella Isolated from Broiler Farms at the Tolima Region—Colombia

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Poultry Research Group, Faculty of Veterinary Medicine, University of Tolima, Altos the Santa Helena, A.A 546, Ibagué 730006299, Colombia
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Investigación y Vigilancia Integrada de la Resistencia Antimicrobiana, Agrosavia, Km 14 Vía Mosquera—Bogotá CI Tibaitatá, Mosquera 250047, Colombia
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Immunobiology and Pathogenesis Research Group, Faculty of Veterinary Medicine, University of Tolima, Altos the Santa Helena, A.A 546, Ibagué 730006299, Colombia
*
Author to whom correspondence should be addressed.
Academic Editor: Morgan B. Farnell
Animals 2021, 11(4), 970; https://doi.org/10.3390/ani11040970
Received: 20 February 2021 / Revised: 8 March 2021 / Accepted: 9 March 2021 / Published: 31 March 2021
(This article belongs to the Special Issue Poultry Microbiology and Immunology)
Salmonella spp. is a major foodborne pathogen with a worldwide distribution that is responsible for salmonellosis in animals and humans. Salmonella contamination of poultry and derivative products occurs at different levels. The aim of this study was to estimate the prevalence of Salmonella in broilers farms and to conduct a phenotypic and molecular characterization of Salmonella isolates. The prevalence at the broiler farm level was 26.67%, and all isolates were found to belong to the serovar Salmonella Paratyphi B. These results suggest a common source of Salmonella contamination between broiler farms, presumably via feed, parent flocks or hatchery machines. Salmonella Paratyphi B is present in different segments of the poultry chain in the Tolima region. Additional studies are needed to identify the main source of Salmonella in broilers, chicken carcasses, and eggs commercialized in the Tolima region.
Salmonella is an important animal and human pathogen responsible for Salmonellosis, and it is frequently associated with the consumption of contaminated poultry products. The aim of this study was to estimate the prevalence of Salmonella in the poultry farms and to determine the genetic relationship. A total of 135 samples collected from fifteen broiler farms, including cloacal, feed, water, environmental and farm operator faeces samples were subjected to microbiological isolation. Molecular confirmation of Salmonella isolates was carried out by amplification of the invA gene, discrimination of d-tartrate-fermenting Salmonella isolates using multiplex PCR, and subsequently analysed by pulsed-field gel electrophoresis (PFGE). A survey questionnaire was conducted to identify potential risk factors for Salmonella presence in broiler farms. The prevalence of Salmonella at the farm level was 26.67%, and Salmonella isolates were serotyped as S. Paratyphi B and all isolates were d-tartrate-fermenting (dT+). PFGE showed three highly similar clusters and one significantly different Salmonella isolate. S. Paratyphi B continued to be present in different links of the poultry chain in the Tolima region, and identification of its main source is necessary to control its dissemination. View Full-Text
Keywords: broilers; Salmonella; PFGE; prevalence; risk factors broilers; Salmonella; PFGE; prevalence; risk factors
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MDPI and ACS Style

Rodríguez-Hernández, R.; Bernal, J.F.; Cifuentes, J.F.; Fandiño, L.C.; Herrera-Sánchez, M.P.; Rondón-Barragán, I.; Verjan Garcia, N. Prevalence and Molecular Characterization of Salmonella Isolated from Broiler Farms at the Tolima Region—Colombia. Animals 2021, 11, 970. https://doi.org/10.3390/ani11040970

AMA Style

Rodríguez-Hernández R, Bernal JF, Cifuentes JF, Fandiño LC, Herrera-Sánchez MP, Rondón-Barragán I, Verjan Garcia N. Prevalence and Molecular Characterization of Salmonella Isolated from Broiler Farms at the Tolima Region—Colombia. Animals. 2021; 11(4):970. https://doi.org/10.3390/ani11040970

Chicago/Turabian Style

Rodríguez-Hernández, Roy, Johan F. Bernal, Jimmy F. Cifuentes, Luz Clemencia Fandiño, María P. Herrera-Sánchez, Iang Rondón-Barragán, and Noel Verjan Garcia. 2021. "Prevalence and Molecular Characterization of Salmonella Isolated from Broiler Farms at the Tolima Region—Colombia" Animals 11, no. 4: 970. https://doi.org/10.3390/ani11040970

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