Frequencies Evaluation of β-Casein Gene Polymorphisms in Dairy Cows Reared in Central Italy
Abstract
Simple Summary
Abstract
1. Introduction
2. Materials and Methods
3. Results and Discussion
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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βcasein Variant | Amino Acid Position | ||||||||
---|---|---|---|---|---|---|---|---|---|
36 | 37 | 67 | 72 | 88 | 93 | 106 | 122 | 138 | |
A2 * | Glu (E) | Glu (E) | Pro (P) | Gln (Q) | Leu (L) | Met (M) | His (H) | Ser (S) | Pro (P) |
A1 * | Glu (E) | Glu (E) | His (H) | Gln (Q) | Leu (L) | Met (M) | His (H) | Ser (S) | Pro (P) |
A3 * | Glu (E) | Glu (E) | Pro (P) | Gln (Q) | Leu (L) | Met (M) | Gln (Q) | Ser (S) | Pro (P) |
B * | Glu (E) | Glu (E) | His (H) | Gln (Q) | Leu (L) | Met (M) | His (H) | Arg (R) | Pro (P) |
C * | Glu (E) | Lys (K) | His (H) | Gln (Q) | Leu (L) | Met (M) | His (H) | Ser (S) | Pro (P) |
E * | Lys (K) | Glu (E) | Pro (P) | Gln (Q) | Leu (L) | Met (M) | His (H) | Ser (S) | Pro (P) |
I * | Glu (E) | Glu (E) | Pro (P) | Gln (Q) | Leu (L) | Leu (L) | His (H) | Ser (S) | Pro (P) |
D | Glu (E) | Glu (E) | Pro (P) | Gln (Q) | Leu (L) | Met (M) | His (H) | Ser (S) | Pro (P) |
F | Glu (E) | Glu (E) | His (H) | Gln (Q) | Leu (L) | Met (M) | His (H) | Ser (S) | Leu (L) |
G | Glu (E) | Glu (E) | His (H) | Gln (Q) | Leu (L) | Met (M) | His (H) | Leu (L) | Pro (P) |
H1 | Glu (E) | Glu (E) | Pro (P) | Gln (Q) | Ile (I) | Met (M) | His (H) | Ser (S) | Pro (P) |
H2 | Glu (E) | Glu (E) | Pro (P) | Glu (E) | Leu (L) | Leu (L) | His (H) | Ser (S) | Glu (E) |
Target Gene | Target Sequence | Primer Sequences | Amplification Product (bp) | Reference |
---|---|---|---|---|
CSN2 | Exon 6 | For CATCAATAAGGTAAAACCCCTCATATT Rev TTGTCAAAGTTTTTATTTCTTGCACTG | 274 | This study |
Exon 7 | For TTTCCAGGATGAACTCCAGGAT Rev CATCAGAAGTTAAACAGGCACAGTTAG | 547 |
Allele | Allele Frequency (%) | Genotype | Genotype Frequency (%) |
---|---|---|---|
A2 | 60.65 | A2/A2 | 36.96 |
A1 | 30.39 | A1/A2 | 35.79 |
B | 5.68 | A1/A1 | 9.88 |
I | 3.10 | A2/B | 7.55 |
A3 | 0.15 | A2/I | 3.93 |
C | 0.03 | A1/B | 3.07 |
A1/I | 2.03 | ||
B/I | 0.25 | ||
B/B | 0.18 | ||
A2/A3 | 0.12 | ||
A3/B | 0.12 | ||
A1/A3 | 0.06 | ||
A1/C | 0.06 |
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Sebastiani, C.; Arcangeli, C.; Ciullo, M.; Torricelli, M.; Cinti, G.; Fisichella, S.; Biagetti, M. Frequencies Evaluation of β-Casein Gene Polymorphisms in Dairy Cows Reared in Central Italy. Animals 2020, 10, 252. https://doi.org/10.3390/ani10020252
Sebastiani C, Arcangeli C, Ciullo M, Torricelli M, Cinti G, Fisichella S, Biagetti M. Frequencies Evaluation of β-Casein Gene Polymorphisms in Dairy Cows Reared in Central Italy. Animals. 2020; 10(2):252. https://doi.org/10.3390/ani10020252
Chicago/Turabian StyleSebastiani, Carla, Chiara Arcangeli, Marcella Ciullo, Martina Torricelli, Giulia Cinti, Stefano Fisichella, and Massimo Biagetti. 2020. "Frequencies Evaluation of β-Casein Gene Polymorphisms in Dairy Cows Reared in Central Italy" Animals 10, no. 2: 252. https://doi.org/10.3390/ani10020252
APA StyleSebastiani, C., Arcangeli, C., Ciullo, M., Torricelli, M., Cinti, G., Fisichella, S., & Biagetti, M. (2020). Frequencies Evaluation of β-Casein Gene Polymorphisms in Dairy Cows Reared in Central Italy. Animals, 10(2), 252. https://doi.org/10.3390/ani10020252