Identification of Bile Salt Hydrolase and Bile Salt Resistance in a Probiotic Bacterium Lactobacillus gasseri JCM1131T
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Strains Used in This Study
2.2. Cloning and Heterologous Expression of a Putative Bile Salt Hydrolase Gene
2.3. Bile Salt Hydrolase Activity
2.4. Biochemical Characterization
2.5. Bile Salt Tolerance Test
2.6. Structural Modeling
2.7. Transcriptional Analysis
3. Results and Discussion
3.1. Identification of BSH Activity and Bile Salt Resistance of Lactobacillus gasseri JCM1131T
3.2. Sequence and Phylogenetic Analyses of a Putative BSH Gene
3.3. Heterologous Expression of the Putative BSH Gene in E. coli
3.4. Bile Salt Hydrolase Assay
3.5. Biochemical Characterization of LagBSH
3.6. Transcriptional Analysis of lagBSH Gene
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Strain | Minimum Inhibitory Concentrations (%) | |||
---|---|---|---|---|
Substrates | ||||
TCA | TDCA | GCA | GDCA | |
JCM1131T | >0.5 | >0.5 | >0.5 | 0.05 |
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Kusada, H.; Morinaga, K.; Tamaki, H. Identification of Bile Salt Hydrolase and Bile Salt Resistance in a Probiotic Bacterium Lactobacillus gasseri JCM1131T. Microorganisms 2021, 9, 1011. https://doi.org/10.3390/microorganisms9051011
Kusada H, Morinaga K, Tamaki H. Identification of Bile Salt Hydrolase and Bile Salt Resistance in a Probiotic Bacterium Lactobacillus gasseri JCM1131T. Microorganisms. 2021; 9(5):1011. https://doi.org/10.3390/microorganisms9051011
Chicago/Turabian StyleKusada, Hiroyuki, Kana Morinaga, and Hideyuki Tamaki. 2021. "Identification of Bile Salt Hydrolase and Bile Salt Resistance in a Probiotic Bacterium Lactobacillus gasseri JCM1131T" Microorganisms 9, no. 5: 1011. https://doi.org/10.3390/microorganisms9051011