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Article

Actinotignum schaalii: Relation to Concomitants and Connection to Patients’ Conditions in Polymicrobial Biofilms of Urinary Tract Catheters and Urines

1
Molecular Genetics Laboratory, Centre for Cardiovascular Surgery and Transplantation, 61600 Brno, Czech Republic
2
Department of Clinical Immunology and Allergology, Medical Faculty, Masaryk University, 61600 Brno, Czech Republic
3
Research Centre for Toxic Compounds in the Environment, Masaryk University, 61600 Brno, Czech Republic
4
Department of Botany and Zoology, Faculty of Science, Masaryk University, 61600 Brno, Czech Republic
5
Institute of Microbiology, Faculty of Medicine, St. Anne’s University Hospital, Masaryk University, 61600 Brno, Czech Republic
*
Author to whom correspondence should be addressed.
Academic Editor: Giovanni Di Bonaventura
Microorganisms 2021, 9(3), 669; https://doi.org/10.3390/microorganisms9030669
Received: 8 February 2021 / Revised: 5 March 2021 / Accepted: 11 March 2021 / Published: 23 March 2021
(This article belongs to the Special Issue Clinical Implications of Microbial Biofilm)
Actinotignum schaalii is an emerging, opportunistic pathogen and its connection to non-infectious diseases and conditions, such as prostate or bladder cancer, or chronic inflammation has been proposed. Here, we analyzed 297 urine, ureteral and urinary catheter samples from 128 patients by Polymerase Chain Reaction followed by Denaturing Gradient Gel Electrophoresis and Sequencing (PCR-DGGE-S), and culture, and 29 of these samples also by 16S rRNA Illumina sequencing, to establish A. schaalii’s prevalence in urinary tract-related samples, its relation to other bacteria, and its potential association with patients’ conditions and samples’ characteristics. A. schaalii-positive samples were significantly more diverse than A. schaalii negative and between-group diversity was higher than intra-group. Propionimicrobium lymphophilum, Fusobacterium nucleatum, Veillonella sp., Morganella sp., and Aerococcus sp. were significantly more often present in A. schaalii-positive samples; thus, we suggest these species are A. schaalii’s concomitants, while Enterobacter and Staphylococcaceae were more often identified in A. schaalii-negative samples; therefore, we propose A. schaalii and these species are mutually exclusive. Additionally, a significantly higher A. schaalii prevalence in patients with ureter stricture associated hydronephrosis (p = 0.020) was noted. We suggest that A. schaalii could be an early polybacterial biofilm colonizer, together with concomitant species, known for pro-inflammatory features. View Full-Text
Keywords: Actinotignum; Actinobaculum; hydronephrosis; urinary catheter; ureteral stent; Double-J catheter; Propionimicrobium; Fusobacterium; urobiome; microbiome; colonisation Actinotignum; Actinobaculum; hydronephrosis; urinary catheter; ureteral stent; Double-J catheter; Propionimicrobium; Fusobacterium; urobiome; microbiome; colonisation
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MDPI and ACS Style

Kotásková, I.; Syrovátka, V.; Obručová, H.; Vídeňská, P.; Zwinsová, B.; Holá, V.; Blaštíková, E.; Růžička, F.; Freiberger, T. Actinotignum schaalii: Relation to Concomitants and Connection to Patients’ Conditions in Polymicrobial Biofilms of Urinary Tract Catheters and Urines. Microorganisms 2021, 9, 669. https://doi.org/10.3390/microorganisms9030669

AMA Style

Kotásková I, Syrovátka V, Obručová H, Vídeňská P, Zwinsová B, Holá V, Blaštíková E, Růžička F, Freiberger T. Actinotignum schaalii: Relation to Concomitants and Connection to Patients’ Conditions in Polymicrobial Biofilms of Urinary Tract Catheters and Urines. Microorganisms. 2021; 9(3):669. https://doi.org/10.3390/microorganisms9030669

Chicago/Turabian Style

Kotásková, Iva, Vít Syrovátka, Hana Obručová, Petra Vídeňská, Barbora Zwinsová, Veronika Holá, Eva Blaštíková, Filip Růžička, and Tomáš Freiberger. 2021. "Actinotignum schaalii: Relation to Concomitants and Connection to Patients’ Conditions in Polymicrobial Biofilms of Urinary Tract Catheters and Urines" Microorganisms 9, no. 3: 669. https://doi.org/10.3390/microorganisms9030669

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