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Article

Isolation of Phaeobacter sp. from Larvae of Atlantic Bonito (Sarda sarda) in a Mesocosmos Unit, and Its Use for the Rearing of European Seabass Larvae (Dicentrarchus labrax L.)

1
Department of Biology, University of Patras, Rio 26504 Patras, Greece
2
Aquaculture and Fisheries Group, Department of Animal Sciences, Wageningen University and Research, 6700AH Wageningen, The Netherlands
3
Biotechnology, and Aquaculture, Hellenic Center for Marine Research, Institute of Marine Biology, P.O. Box 2214, 71003 Heraklion Crete, Greece
*
Author to whom correspondence should be addressed.
Microorganisms 2021, 9(1), 128; https://doi.org/10.3390/microorganisms9010128
Received: 26 November 2020 / Revised: 24 December 2020 / Accepted: 6 January 2021 / Published: 8 January 2021
(This article belongs to the Special Issue Animal Microbiota: Securing Optimal Gene-Diet-Microbiota Interactions)
The target of this study was to use indigenous probiotic bacteria in the rearing of seabass larvae. A Phaeobacter sp. strain isolated from bonito yolk-sac larvae (Sarda sarda) and identified by amplification of 16S rDNA showed in vitro inhibition against Vibrio anguillarum. This Phaeobacter sp. strain was used in the rearing of seabass larvae (Dicentrarchus labrax L.) in a large-scale trial. The survival of seabass after 60 days of rearing and the specific growth rate at the late exponential growth phase were significantly higher in the treatment receiving probiotics (p < 0.05). Microbial community richness as determined by denaturing gradient gel electrophoresis (DGGE) showed an increase in bacterial diversity with fish development. Changes associated with the administration of probiotics were observed 11 and 18 days after hatching but were not apparent after probiotic administration stopped. In a small challenge experiment, seabass larvae from probiotic treatment showed increased survival (p < 0.05) after experimental infection with a mild pathogen (Vibrio harveyi). Overall, our results showed that the use of an indigenous probiotic strain had a beneficial impact on larval rearing in industry-like conditions. View Full-Text
Keywords: larviculture; fish microbiota; antagonism; microbiology; challenge test larviculture; fish microbiota; antagonism; microbiology; challenge test
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MDPI and ACS Style

Makridis, P.; Kokou, F.; Bournakas, C.; Papandroulakis, N.; Sarropoulou, E. Isolation of Phaeobacter sp. from Larvae of Atlantic Bonito (Sarda sarda) in a Mesocosmos Unit, and Its Use for the Rearing of European Seabass Larvae (Dicentrarchus labrax L.). Microorganisms 2021, 9, 128. https://doi.org/10.3390/microorganisms9010128

AMA Style

Makridis P, Kokou F, Bournakas C, Papandroulakis N, Sarropoulou E. Isolation of Phaeobacter sp. from Larvae of Atlantic Bonito (Sarda sarda) in a Mesocosmos Unit, and Its Use for the Rearing of European Seabass Larvae (Dicentrarchus labrax L.). Microorganisms. 2021; 9(1):128. https://doi.org/10.3390/microorganisms9010128

Chicago/Turabian Style

Makridis, Pavlos, Fotini Kokou, Christos Bournakas, Nikos Papandroulakis, and Elena Sarropoulou. 2021. "Isolation of Phaeobacter sp. from Larvae of Atlantic Bonito (Sarda sarda) in a Mesocosmos Unit, and Its Use for the Rearing of European Seabass Larvae (Dicentrarchus labrax L.)" Microorganisms 9, no. 1: 128. https://doi.org/10.3390/microorganisms9010128

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