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Brief Report

Human Cytomegalovirus Infection Suppresses CD34+ Progenitor Cell Engraftment in Humanized Mice

1
Vaccine and Gene Therapy Institute, Oregon Health & Science University, Beaverton, OR 97006, USA
2
Department of Microbiology and Immunology, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA
3
Department of Immunobiology, BIO5 Institute, University of Arizona, Tucson, AZ 85719, USA
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(4), 525; https://doi.org/10.3390/microorganisms8040525
Received: 10 March 2020 / Revised: 3 April 2020 / Accepted: 4 April 2020 / Published: 6 April 2020
(This article belongs to the Special Issue Cytomegalovirus: Biology and Infection)
Human cytomegalovirus (HCMV) infection is a serious complication in hematopoietic stem cell transplant (HSCT) recipients due to virus-induced myelosuppression and impairment of stem cell engraftment. Despite the clear clinical link between myelosuppression and HCMV infection, little is known about the mechanism(s) by which the virus inhibits normal hematopoiesis because of the strict species specificity and the lack of surrogate animal models. In this study, we developed a novel humanized mouse model system that recapitulates the HCMV-mediated engraftment failure after hematopoietic cell transplantation. We observed significant alterations in the hematopoietic populations in peripheral lymphoid tissues following engraftment of a subset of HCMV+ CD34+ hematopoietic progenitor cells (HPCs) within the transplant, suggesting that a small proportion of HCMV-infected CD34+ HPCs can profoundly affect HPC differentiation in the bone marrow microenvironment. This model will be instrumental to gain insight into the fundamental mechanisms of HCMV myelosuppression after HSCT and provides a platform to assess novel treatment strategies. View Full-Text
Keywords: human cytomegalovirus; progenitor cell; hematopoietic stem cell transplant; myelosuppression; hematopoiesis; humanized mice human cytomegalovirus; progenitor cell; hematopoietic stem cell transplant; myelosuppression; hematopoiesis; humanized mice
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MDPI and ACS Style

Crawford, L.B.; Tempel, R.; Streblow, D.N.; Yurochko, A.D.; Goodrum, F.D.; Nelson, J.A.; Caposio, P. Human Cytomegalovirus Infection Suppresses CD34+ Progenitor Cell Engraftment in Humanized Mice. Microorganisms 2020, 8, 525. https://doi.org/10.3390/microorganisms8040525

AMA Style

Crawford LB, Tempel R, Streblow DN, Yurochko AD, Goodrum FD, Nelson JA, Caposio P. Human Cytomegalovirus Infection Suppresses CD34+ Progenitor Cell Engraftment in Humanized Mice. Microorganisms. 2020; 8(4):525. https://doi.org/10.3390/microorganisms8040525

Chicago/Turabian Style

Crawford, Lindsey B., Rebecca Tempel, Daniel N. Streblow, Andrew D. Yurochko, Felicia D. Goodrum, Jay A. Nelson, and Patrizia Caposio. 2020. "Human Cytomegalovirus Infection Suppresses CD34+ Progenitor Cell Engraftment in Humanized Mice" Microorganisms 8, no. 4: 525. https://doi.org/10.3390/microorganisms8040525

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