Next Article in Journal
The Uropathogenic Specific Protein Gene usp from Escherichia coli and Salmonella bongori is a Novel Member of the TyrR and H-NS Regulons
Previous Article in Journal
Impact of Cover Crops on the Soil Microbiome of Tree Crops
Previous Article in Special Issue
Safety Aspects of Genetically Modified Lactic Acid Bacteria
Article

Whole-Genome Sequencing of Lactobacillus helveticus D75 and D76 Confirms Safety and Probiotic Potential

1
Department of Microbiology, State Research Institute of Highly Pure Biopreparations, 197110 St. Petersburg, Russia
2
Department of Internal Diseases, Gastroenterology and Dietetics, North-Western State Medical University named after I.I. Mechnikov, 195067 St. Petersburg, Russia
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(3), 329; https://doi.org/10.3390/microorganisms8030329
Received: 24 December 2019 / Revised: 14 February 2020 / Accepted: 24 February 2020 / Published: 26 February 2020
(This article belongs to the Special Issue Safety Aspects of Lactic Acid Bacteria)
Whole-genome DNA sequencing of Lactobacillus D75 and D76 strains (Vitaflor, Russia) was determined using the PacBio RS II platform, which was followed by de novo assembly with SMRT Portal 2.3.0. The average nucleotide identity (ANI) test showed that both strains belong to the Lactobacillus helveticus, but not to the L. acidophilus, as previously assumed. In addition, 31 exopolysaccharide (EPS) production genes (nine of which form a single genetic cluster), 13 adhesion genes, 38 milk protein and 11 milk sugar utilization genes, 13 genes for and against specific antagonistic activity, eight antibiotic resistance genes, and also three CRISPR blocks and eight Cas I-B system genes were identified in the genomes of both strains. The expression of bacteriocin helveticin J genes was confirmed. In fact, the presence of identified genes suggests that L. helveticus D75 and D76 are able to form biofilms on the outer mucin layer, inhibit the growth of pathogens and pathobionts, utilize milk substrates with the formation of digestible milk sugars and bioactive peptides, resist bacteriophages, show some genome-determined resistance to antibiotics, and stimulate the host’s immune system. Pathogenicity genes have not been identified. The study results confirm the safety and high probiotic potential of the strains. View Full-Text
Keywords: Lactobacillus helveticus; probiotics; whole genome sequencing; PacBio; probiotic genes; bacteriocins; gene expression Lactobacillus helveticus; probiotics; whole genome sequencing; PacBio; probiotic genes; bacteriocins; gene expression
Show Figures

Figure 1

MDPI and ACS Style

Toropov, V.; Demyanova, E.; Shalaeva, O.; Sitkin, S.; Vakhitov, T. Whole-Genome Sequencing of Lactobacillus helveticus D75 and D76 Confirms Safety and Probiotic Potential. Microorganisms 2020, 8, 329. https://doi.org/10.3390/microorganisms8030329

AMA Style

Toropov V, Demyanova E, Shalaeva O, Sitkin S, Vakhitov T. Whole-Genome Sequencing of Lactobacillus helveticus D75 and D76 Confirms Safety and Probiotic Potential. Microorganisms. 2020; 8(3):329. https://doi.org/10.3390/microorganisms8030329

Chicago/Turabian Style

Toropov, Vyacheslav; Demyanova, Elena; Shalaeva, Olga; Sitkin, Stanislav; Vakhitov, Timur. 2020. "Whole-Genome Sequencing of Lactobacillus helveticus D75 and D76 Confirms Safety and Probiotic Potential" Microorganisms 8, no. 3: 329. https://doi.org/10.3390/microorganisms8030329

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop