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Open AccessArticle

DNA Methylation Changes Induced by Cold in Psychrophilic and Psychrotolerant Naganishia Yeast Species

Department of Agricultural, Food and Environmental Sciences, University of Perugia, Borgo XX Giugno, 74, 06121 Perugia, Italy
*
Author to whom correspondence should be addressed.
Contributed equally to this work.
Microorganisms 2020, 8(2), 296; https://doi.org/10.3390/microorganisms8020296
Received: 15 January 2020 / Revised: 11 February 2020 / Accepted: 14 February 2020 / Published: 20 February 2020
(This article belongs to the Special Issue Non-Conventional Yeasts)
The involvement of DNA methylation in the response to cold stress of two different yeast species (Naganishia antarctica, psychrophilic, and Naganishia albida, psychrotolerant), exhibiting different temperature aptitudes, has been studied. Consecutive incubations at respective optimum temperatures, at 4 °C (cold stress) and at optimum temperatures again, were performed. After Methylation Sensitive Amplified Polymorphism (MSAP) fingerprints a total of 550 and 423 clear and reproducible fragments were amplified from N. antarctica and N. albida strains, respectively. The two Naganishia strains showed a different response in terms of level of DNA methylation during cold stress and recovery from cold stress. The percentage of total methylated fragments in psychrophilic N. antarctica did not show any significant change. On the contrary, the methylation of psychrotolerant N. albida exhibited a nonsignificant increase during the incubation at 4 °C and continued during the recovery step, showing a significant difference if compared with control condition, resembling an uncontrolled response to cold stress. A total of 12 polymorphic fragments were selected, cloned, and sequenced. Four fragments were associated to genes encoding for elongation factor G and for chitin synthase export chaperon. To the best of our knowledge, this is the first study on DNA methylation in the response to cold stress carried out by comparing a psychrophilic and a psychrotolerant yeast species. View Full-Text
Keywords: cold-adapted yeasts; cold stress response; Naganishia albida; Naganishia antarctica cold-adapted yeasts; cold stress response; Naganishia albida; Naganishia antarctica
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Turchetti, B.; Marconi, G.; Sannino, C.; Buzzini, P.; Albertini, E. DNA Methylation Changes Induced by Cold in Psychrophilic and Psychrotolerant Naganishia Yeast Species. Microorganisms 2020, 8, 296.

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