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Open AccessArticle

The Microbiota Profile in Inflamed and Non-Inflamed Ileal Pouch–Anal Anastomosis

1
Department of Gastrointestinal Surgery, Aalborg University Hospital, Hobrovej 18-22, 9000 Aalborg, Denmark
2
Department of Clinical Medicine, Aalborg University, Søndre Skovvej 15, 9000 Aalborg, Denmark
3
Center for Microbial Communities, Aalborg University, Frederik Bajers Vej 7H, 9220 Aalborg, Denmark
4
Department of Clinical Microbiology, Aalborg University Hospital, Mølleparkvej 10, 9000 Aalborg, Denmark
5
Department of Infectious Disease, Aalborg University Hospital, Mølleparkvej 4, 9000 Aalborg, Denmark
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(10), 1611; https://doi.org/10.3390/microorganisms8101611
Received: 21 September 2020 / Revised: 13 October 2020 / Accepted: 19 October 2020 / Published: 20 October 2020
(This article belongs to the Section Gut Microbiota)
The objective was to determine the bacterial composition in inflamed and non-inflamed pouches for comparison to the microbiota of healthy individuals. Pouch patients and healthy individuals were included between November 2017 and June 2019 at the Department of Gastrointestinal Surgery, Aalborg University Hospital, Denmark. A faecal sample was collected from all participants for microbiota analysis using 16S rRNA amplicon sequencing. Overall, 38 participants were included in the study. Eleven patients with a normally functioning pouch, 9 patients with chronic pouchitis, 6 patients with familial adenomatous polyposis, and 12 healthy individuals. Patients with chronic pouchitis had overall lower microbial diversity and richness compared to patients with a normal pouch function (p < 0.001 and p = 0.009) and healthy individuals (p < 0.001 and p < 0.001). No significant difference was found between patients with familial adenomatous polyposis and chronic pouchitis (microbial diversity p = 0.39 and richness p = 0.78). Several taxa from the family Enterobacteriaceae, especially genus Escherichia, were associated primarily with patients with chronic pouchitis, while taxa from the genus Bacteroides primarily were associated with healthy individuals and patients with a normally functioning pouch. Finally, a microbial composition gradient could be established from healthy individuals through patients with normal pouch function and familial adenomatous polyposis to patients with chronic pouchitis. View Full-Text
Keywords: Bacteroides; dysbiosis; Escherichia; Enterobacteriaceae; FAP; IPAA; microbiota; pouchitis Bacteroides; dysbiosis; Escherichia; Enterobacteriaceae; FAP; IPAA; microbiota; pouchitis
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MDPI and ACS Style

Kousgaard, S.J.; Michaelsen, T.Y.; Nielsen, H.L.; Kirk, K.F.; Albertsen, M.; Thorlacius-Ussing, O. The Microbiota Profile in Inflamed and Non-Inflamed Ileal Pouch–Anal Anastomosis. Microorganisms 2020, 8, 1611. https://doi.org/10.3390/microorganisms8101611

AMA Style

Kousgaard SJ, Michaelsen TY, Nielsen HL, Kirk KF, Albertsen M, Thorlacius-Ussing O. The Microbiota Profile in Inflamed and Non-Inflamed Ileal Pouch–Anal Anastomosis. Microorganisms. 2020; 8(10):1611. https://doi.org/10.3390/microorganisms8101611

Chicago/Turabian Style

Kousgaard, Sabrina J.; Michaelsen, Thomas Y.; Nielsen, Hans L.; Kirk, Karina F.; Albertsen, Mads; Thorlacius-Ussing, Ole. 2020. "The Microbiota Profile in Inflamed and Non-Inflamed Ileal Pouch–Anal Anastomosis" Microorganisms 8, no. 10: 1611. https://doi.org/10.3390/microorganisms8101611

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