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Experimental Challenges for Reduced Genomes: The Cell Model Escherichia coli

Graduate School of Life and Environmental Sciences, University of Tsukuba, 305-8572 Ibaraki, Japan
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Microorganisms 2020, 8(1), 3; https://doi.org/10.3390/microorganisms8010003
Received: 25 November 2019 / Revised: 11 December 2019 / Accepted: 17 December 2019 / Published: 18 December 2019
(This article belongs to the Section Systems Microbiology)
Genome reduction, as a top-down approach to obtain the minimal genetic information essential for a living organism, has been conducted with bacterial cells for decades. The most popular and well-studied cell models for genome reduction are Escherichia coli strains. As the previous literature intensively introduced the genetic construction and application of the genome-reduced Escherichia coli strains, the present review focuses the design principles and compares the reduced genome collections from the specific viewpoint of growth, which represents a fundamental property of living cells and is an important feature for their biotechnological application. For the extended simplification of the genomic sequences, the approach of experimental evolution and concern for medium optimization are newly proposed. The combination of the current techniques of genomic construction and the newly proposed methodologies could allow us to acquire growing Escherichia coli cells carrying the extensively reduced genome and to address the question of what the minimal genome essential for life is. View Full-Text
Keywords: genome reduction; growth rate; experimental evolution; minimal genome; Escherichia coli genome reduction; growth rate; experimental evolution; minimal genome; Escherichia coli
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MDPI and ACS Style

Kurokawa, M.; Ying, B.-W. Experimental Challenges for Reduced Genomes: The Cell Model Escherichia coli. Microorganisms 2020, 8, 3. https://doi.org/10.3390/microorganisms8010003

AMA Style

Kurokawa M, Ying B-W. Experimental Challenges for Reduced Genomes: The Cell Model Escherichia coli. Microorganisms. 2020; 8(1):3. https://doi.org/10.3390/microorganisms8010003

Chicago/Turabian Style

Kurokawa, Masaomi; Ying, Bei-Wen. 2020. "Experimental Challenges for Reduced Genomes: The Cell Model Escherichia coli" Microorganisms 8, no. 1: 3. https://doi.org/10.3390/microorganisms8010003

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