Next Article in Journal
Reanalysis of Lactobacillus paracasei Lbs2 Strain and Large-Scale Comparative Genomics Places Many Strains into Their Correct Taxonomic Position
Next Article in Special Issue
Comparative Transcriptomic Analysis of the Interaction between Penicillium expansum and Apple Fruit (Malus pumila Mill.) during Early Stages of Infection
Previous Article in Journal
Microbial Community Structure and Methane Cycling Potential along a Thermokarst Pond-Peatland Continuum
Previous Article in Special Issue
Identification and Toxigenic Potential of Fungi Isolated from Capsicum Peppers
Open AccessArticle

Elucidation of the Initial Growth Process and the Infection Mechanism of Penicillium digitatum on Postharvest Citrus (Citrus reticulata Blanco)

1
School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China
2
Nanjing Dongshan Foreign Language School, 99 Shanggao Road, Jiangning District, Nanjing 211103, China
*
Author to whom correspondence should be addressed.
The authors contributed equally to this study.
Microorganisms 2019, 7(11), 485; https://doi.org/10.3390/microorganisms7110485
Received: 24 September 2019 / Revised: 18 October 2019 / Accepted: 21 October 2019 / Published: 24 October 2019
(This article belongs to the Special Issue Interplay between Fungal Pathogens and Harvested Crops)
Green mold disease, a common citrus post-harvest disease caused by Penicillium digitatum, has an unresolved initial infection mechanism. Understanding the infection mechanism leads to the development of potential controls and preventive measures against the disease. The present study aimed to delineate the infection mechanism by investigating spore germination, changes of organic molecules and enzyme activity, and differential expression of genes in the P. digitatum infection. P. digitatum spore germination was observed by a pathology section scanner and it was found that in vivo germination was 3 h behind the in vitro germination. In addition, cell wall degrading enzymes and soluble sugar and titratable acid content during the infection process measured dynamically. The level of pectinase reached its maximum of 6067 U/g before 48 hpi, while cellulase increased rapidly after 48 hpi. The soluble sugar and organic acid content increased considerably with the progression of the infection. The transcriptomic profile of P. digitatum before and after infection was analyzed by RNA-seq. The genes related to cell wall degrading enzymes were significantly up-regulated and annotated to participate in two major carbon source synthesis pathways. The study delineated the initial infection mechanism of P. digitatum which eventually opened the gate way for the development of new control strategies in the future. View Full-Text
Keywords: Penicillium digitatum; citrus; initial process; cell wall degrading enzyme; RNA-seq Penicillium digitatum; citrus; initial process; cell wall degrading enzyme; RNA-seq
Show Figures

Figure 1

MDPI and ACS Style

Qian, X.; Yang, Q.; Zhang, Q.; Abdelhai, M.H.; Dhanasekaran, S.; Serwah, B.N.A.; Gu, N.; Zhang, H. Elucidation of the Initial Growth Process and the Infection Mechanism of Penicillium digitatum on Postharvest Citrus (Citrus reticulata Blanco). Microorganisms 2019, 7, 485.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop