Seroprevalence Study of Conserved Enterotoxigenic Escherichia coli Antigens in Globally Diverse Populations
Abstract
:1. Introduction
2. Materials and Methods
2.1. Recombinant Antigen Production
2.2. Human Serum Samples
2.3. ELISA
2.4. Statistical Analysis
2.5. Ethics Statement
3. Results
3.1. Seroprevalence
3.2. Immunogenicity
4. Discussion
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Antigen | Isotype | Antigen | ||||
---|---|---|---|---|---|---|
Present | Absent | |||||
Change * | N | Change | N | p-Value | ||
EtpA | IgG | 0.13 | 6 | 0.12 | 119 | 0.891 |
IgA | 0.04 | 0.14 | 0.689 | |||
EatA | IgG | 0.08 | 6 | −0.05 | 119 | 0.280 |
IgA | 0.32 | 0.01 | 0.034 | |||
LT | IgG | 0.20 | 20 | −0.02 | 105 | 0.006 |
IgA | −0.02 | −0.05 | 0.771 |
Age | Symptoms | Antigen | Symptoms:Antigen * | |||
---|---|---|---|---|---|---|
EtpA IgG | 0.074 | p-value | 0.008 | 0.081 | 0.945 | 0.045 |
η2 | 0.058 | 0.025 | 0.000 | 0.033 | ||
EtpA IgA | 0.046 | p-value | 0.021 | 0.463 | 0.641 | 0.347 |
η2 | 0.043 | 0.004 | 0.002 | 0.007 | ||
EatA IgG † | 0.064 | p-value | 0.013 | 0.567 | 0.429 | 0.709 |
η2 | 0.051 | 0.003 | 0.005 | 0.001 | ||
EatA IgA | 0.072 | p-value | 0.107 | 0.232 | 0.047 | 0.405 |
η2 | 0.021 | 0.012 | 0.033 | 0.006 | ||
LT IgG | 0.223 | p-value | 0.010 | 0.001 | 0.001 | 0.494 |
η2 | 0.054 | 0.096 | 0.088 | 0.004 | ||
LT IgA † | 0.093 | p-value | 0.630 | 0.001 | 0.363 | 0.006 |
η2 | 0.002 | 0.081 | 0.007 | 0.062 |
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Kuhlmann, F.M.; Grigura, V.; Vickers, T.J.; Prouty, M.G.; Iannotti, L.L.; Dulience, S.J.L.; Fleckenstein, J.M. Seroprevalence Study of Conserved Enterotoxigenic Escherichia coli Antigens in Globally Diverse Populations. Microorganisms 2023, 11, 2221. https://doi.org/10.3390/microorganisms11092221
Kuhlmann FM, Grigura V, Vickers TJ, Prouty MG, Iannotti LL, Dulience SJL, Fleckenstein JM. Seroprevalence Study of Conserved Enterotoxigenic Escherichia coli Antigens in Globally Diverse Populations. Microorganisms. 2023; 11(9):2221. https://doi.org/10.3390/microorganisms11092221
Chicago/Turabian StyleKuhlmann, Frederick Matthew, Vadim Grigura, Timothy J. Vickers, Michael G. Prouty, Lora L. Iannotti, Sherlie Jean Louis Dulience, and James M. Fleckenstein. 2023. "Seroprevalence Study of Conserved Enterotoxigenic Escherichia coli Antigens in Globally Diverse Populations" Microorganisms 11, no. 9: 2221. https://doi.org/10.3390/microorganisms11092221
APA StyleKuhlmann, F. M., Grigura, V., Vickers, T. J., Prouty, M. G., Iannotti, L. L., Dulience, S. J. L., & Fleckenstein, J. M. (2023). Seroprevalence Study of Conserved Enterotoxigenic Escherichia coli Antigens in Globally Diverse Populations. Microorganisms, 11(9), 2221. https://doi.org/10.3390/microorganisms11092221