Next Article in Journal
Antimicrobial Activity of Six Essential Oils Against a Group of Human Pathogens: A Comparative Study
Previous Article in Journal
Disease Manifestation and Viral Sequences in a Bonobo More Than 30 Years after Papillomavirus Infection
Article

Motility, Biofilm Formation and Antimicrobial Efflux of Sessile and Planktonic Cells of Achromobacter xylosoxidans

1
Department of Clinical Medicine, Health, Aarhus University, DK-8200 Aarhus, Denmark
2
Department of Clinical Microbiology, Aarhus University Hospital, DK-8200 Aarhus, Denmark
*
Author to whom correspondence should be addressed.
Pathogens 2019, 8(1), 14; https://doi.org/10.3390/pathogens8010014
Received: 19 December 2018 / Revised: 18 January 2019 / Accepted: 24 January 2019 / Published: 27 January 2019
(This article belongs to the Section Human Pathogens)
Achromobacter xylosoxidans is an innately multidrug-resistant bacterium capable of forming biofilms in the respiratory tract of cystic fibrosis (CF) patients. During the transition from the planktonic stage to biofilm growth, bacteria undergo a transcriptionally regulated differentiation. An isolate of A. xylosoxidans cultured from the sputum of a CF patient was separated into sessile and planktonic stages in vitro, and the transcriptomes were compared. The selected genes of interest were subsequently inactivated, and flagellar motility was found to be decisive for biofilm formation in vitro. The spectrum of a new resistance-nodulation-cell division (RND)-type multidrug efflux pump (AxyEF-OprN) was characterized by inactivation of the membrane fusion protein. AxyEF-OprN is capable of extruding some fluoroquinolones (levofloxacin and ciprofloxacin), tetracyclines (doxycycline and tigecycline) and carpabenems (ertapenem and imipenem), which are classes of antimicrobials that are widely used for treatment of CF pulmonary infections. View Full-Text
Keywords: Achromobacter xylosoxidans; cystic fibrosis; transcriptomics; biofilm; antimicrobial efflux Achromobacter xylosoxidans; cystic fibrosis; transcriptomics; biofilm; antimicrobial efflux
Show Figures

Figure 1

MDPI and ACS Style

Nielsen, S.M.; Penstoft, L.N.; Nørskov-Lauritsen, N. Motility, Biofilm Formation and Antimicrobial Efflux of Sessile and Planktonic Cells of Achromobacter xylosoxidans. Pathogens 2019, 8, 14. https://doi.org/10.3390/pathogens8010014

AMA Style

Nielsen SM, Penstoft LN, Nørskov-Lauritsen N. Motility, Biofilm Formation and Antimicrobial Efflux of Sessile and Planktonic Cells of Achromobacter xylosoxidans. Pathogens. 2019; 8(1):14. https://doi.org/10.3390/pathogens8010014

Chicago/Turabian Style

Nielsen, Signe M., Line N. Penstoft, and Niels Nørskov-Lauritsen. 2019. "Motility, Biofilm Formation and Antimicrobial Efflux of Sessile and Planktonic Cells of Achromobacter xylosoxidans" Pathogens 8, no. 1: 14. https://doi.org/10.3390/pathogens8010014

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop