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Preparation of Poly (dl-Lactide-co-Glycolide) Nanoparticles Encapsulated with Periglaucine A and Betulinic Acid for In Vitro Anti-Acanthamoeba and Cytotoxicity Activities

1
Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia
2
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia
3
Department of Nano-Medicine Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi Arabia
4
School of Pharmacy, Nottingham University Malaysia Campus, Semenyih, Selangor, Kuala Lumpur 43500, Malaysia
5
School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80161, Thailand
6
Research Excellence Center for Innovation and Health Products, Walailak University, Nakhon Si Thammarat 80161, Thailand
*
Authors to whom correspondence should be addressed.
Pathogens 2018, 7(3), 62; https://doi.org/10.3390/pathogens7030062
Received: 19 June 2018 / Revised: 9 July 2018 / Accepted: 12 July 2018 / Published: 16 July 2018
Poly (dl-lactide-co-glycolide) (PLGA) microspheres were synthesized as delivery system for the natural anti-parasitic compounds, Periglaucine A (PGA) and Betulinic acid (BA). Periglaucine A and Betulinic acid were encapsulated in PLGA nanoparticles by single emulsion method with an average particle size of approximately 100–500 nm. Periglaucine A and Betulinic acid encapsulation efficiency was observed to be 90% and 35% respectively. Anti-Acanthamoeba property of Periglaucine A and Betulinic acid remained intact after encapsulation. PGA-PLGA and BA-PLGA nanoparticles demonstrated inhibition in viability of Acanthamoeba triangularis trophozoites by 74.9%, 59.9%, 49.9% and 71.2%, 52.2%, 88% respectively at concentration of 100 µg/mL, 50 µg/mL and 25 µg/mL. Cytotoxicity of PGA-PLGA and BA-PLGA nanoparticles has been evaluated against lung epithelial cell line and showed dose dependent cytotoxicity value of IC50 2 µg/mL and 20 µg/mL respectively. Futher, increased viability was observed in lung epithelial cell line in higher doses of synthesized polymeric nanoparticles. Results indicate that poly (dl-lactide-co-glycolide) (PLGA) nanoparticles could be exploratory delivery systems for natural products to improve their therapeutic efficacy. View Full-Text
Keywords: anti-Acanthamoeba; betulinic acid; cytotoxicity; periglaucine A; PLGA-nanoparticles anti-Acanthamoeba; betulinic acid; cytotoxicity; periglaucine A; PLGA-nanoparticles
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MDPI and ACS Style

Mahboob, T.; Nawaz, M.; Tian-Chye, T.; Samudi, C.; Wiart, C.; Nissapatorn, V. Preparation of Poly (dl-Lactide-co-Glycolide) Nanoparticles Encapsulated with Periglaucine A and Betulinic Acid for In Vitro Anti-Acanthamoeba and Cytotoxicity Activities. Pathogens 2018, 7, 62.

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