Effect of Simultaneous Exposure of Pigs to Streptococcus suis Serotypes 2 and 9 on Their Colonization and Transmission, and on Mortality
Abstract
:1. Introduction
2. Results
2.1. Colonization
2.2. Transmission
2.3. Clinical Disease and Mortality
3. Discussion
4. Materials and Methods
4.1. Inoculum
4.2. Pigs
4.3. Experimental Design
4.4. Clinical Inspection
4.5. Sampling
4.6. Laboratory Tests
4.6.1. Bacterial Examination
4.6.2. Multiplex Real-Time Quantitative PCR
4.7. Statistical Analysis
4.7.1. S. suis Colonization
4.7.2. Animal Survival
4.7.3. Transmission
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Inoculated Pigs | Contact Exposed Pigs | |||
---|---|---|---|---|
Time (DPE) 1 | Mean load ‘Mono’ Minus Mean Load ‘Dual’ (log10 eq. CFU/sample) 2,3 | p-Value | Mean load ‘Mono’ Minus Mean Load ‘Dual’ (log10 eq. CFU/sample) 2,3 | p-Value |
1 | 0.64 | 0.352 | 1.57 | <0.001 |
2 | 0.46 | 0.873 | 1.40 | 0.004 |
3 | 0.79 | 0.158 | 1.72 | <0.001 |
4 | 0.82 | 0.158 | 1.76 | <0.001 |
5 | −0.06 | 0.999 | 0.87 | 0.351 |
6 | 0.55 | 0.999 | 1.48 | 0.012 |
7 | 0.31 | 0.999 | 1.24 | 0.078 |
8 | −0.64 | 0.999 | 0.29 | 0.999 |
Inoculated Pigs | Contact Exposed Pigs | |||
---|---|---|---|---|
Time (DPE) 1 | Mean load ‘Mono’ Minus Mean Load ‘Dual’ (log10 eq. CFU/sample) 2,3 | p-Value | Mean load ‘Mono’ Minus Mean Load ‘Dual’ (log10 eq. CFU/sample) 2,3 | p-Value |
1 | 1.61 | 0.004 | 1.72 | <0.001 |
2 | 0.86 | 0.361 | 0.96 | 0.240 |
3 | 0.79 | 0.361 | 0.90 | 0.227 |
4 | 0.29 | 0.999 | 0.40 | 0.999 |
5 | 0.00 | 0.999 | 0.00 | 0.999 |
6 | 0.00 | 0.999 | 0.09 | 0.999 |
7 | 0.13 | 0.999 | 0.24 | 0.999 |
8 | 0.03 | 0.999 | 0.13 | 0.999 |
Inoculated Pigs | Contact Exposed Pigs | |||
---|---|---|---|---|
Time (DPE) 1 | Mean load ‘Mono’ Minus Mean Load ‘Dual’ (log10 eq. CFU/sample) 2,3 | p-Value | Mean load ‘Mono’ Minus Mean Load ‘Dual’ (log10 eq. CFU/sample) 2,3 | p-Value |
1 | 0.94 | 0.233 | 0.67 | 0.709 |
2 | 0.98 | 0.203 | 0.71 | 0.620 |
3 | 0.72 | 0.650 | 0.45 | 0.999 |
4 | 0.48 | 0.999 | 0.21 | 0.999 |
Inoculated Pigs | Contact Exposed Pigs | |||
---|---|---|---|---|
Time (DPE) 1 | Mean load ‘Mono’ Minus Mean Load ‘Dual’ (log10 eq. CFU/sample) 2,3 | p-Value | Mean load ‘Mono’ Minus Mean Load ‘Dual’ (log10 eq. CFU/sample) 2,3 | p-Value |
1 | 0.73 | 0.999 | 1.29 | 0.352 |
2 | 1.63 | 0.157 | 2.19 | 0.013 |
3 | 1.15 | 0.566 | 1.70 | 0.133 |
4 | 1.06 | 0.747 | 1.61 | 0.170 |
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Dekker, N.; Bouma, A.; Daemen, I.; Vernooij, H.; Van Leengoed, L.; Wagenaar, J.A.; Stegeman, A. Effect of Simultaneous Exposure of Pigs to Streptococcus suis Serotypes 2 and 9 on Their Colonization and Transmission, and on Mortality. Pathogens 2017, 6, 46. https://doi.org/10.3390/pathogens6040046
Dekker N, Bouma A, Daemen I, Vernooij H, Van Leengoed L, Wagenaar JA, Stegeman A. Effect of Simultaneous Exposure of Pigs to Streptococcus suis Serotypes 2 and 9 on Their Colonization and Transmission, and on Mortality. Pathogens. 2017; 6(4):46. https://doi.org/10.3390/pathogens6040046
Chicago/Turabian StyleDekker, Niels, Annemarie Bouma, Ineke Daemen, Hans Vernooij, Leo Van Leengoed, Jaap A. Wagenaar, and Arjan Stegeman. 2017. "Effect of Simultaneous Exposure of Pigs to Streptococcus suis Serotypes 2 and 9 on Their Colonization and Transmission, and on Mortality" Pathogens 6, no. 4: 46. https://doi.org/10.3390/pathogens6040046
APA StyleDekker, N., Bouma, A., Daemen, I., Vernooij, H., Van Leengoed, L., Wagenaar, J. A., & Stegeman, A. (2017). Effect of Simultaneous Exposure of Pigs to Streptococcus suis Serotypes 2 and 9 on Their Colonization and Transmission, and on Mortality. Pathogens, 6(4), 46. https://doi.org/10.3390/pathogens6040046