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Review

WGS Analysis and Interpretation in Clinical and Public Health Microbiology Laboratories: What Are the Requirements and How Do Existing Tools Compare?

1
IBM Research—Australia, Level 5, 204 Lygon Street, Carlton, Victoria 3206, Australia
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IBM Research—Zuerich, Säumerstrasse 4, Rüschlikon 8803, Switzerland
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Bio21 Institute, University of Melbourne, 30 Flemington Road, Melbourne, Parkville, VIC 3052, Australia
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Author to whom correspondence should be addressed.
Pathogens 2014, 3(2), 437-458; https://doi.org/10.3390/pathogens3020437
Received: 24 April 2014 / Revised: 30 May 2014 / Accepted: 3 June 2014 / Published: 11 June 2014
Recent advances in DNA sequencing technologies have the potential to transform the field of clinical and public health microbiology, and in the last few years numerous case studies have demonstrated successful applications in this context. Among other considerations, a lack of user-friendly data analysis and interpretation tools has been frequently cited as a major barrier to routine use of these techniques. Here we consider the requirements of microbiology laboratories for the analysis, clinical interpretation and management of bacterial whole-genome sequence (WGS) data. Then we discuss relevant, existing WGS analysis tools. We highlight many essential and useful features that are represented among existing tools, but find that no single tool fulfils all of the necessary requirements. We conclude that to fully realise the potential of WGS analyses for clinical and public health microbiology laboratories of all scales, we will need to develop tools specifically with the needs of these laboratories in mind. View Full-Text
Keywords: genomics; microbiology; clinical; public health; bioinformatics genomics; microbiology; clinical; public health; bioinformatics
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MDPI and ACS Style

Wyres, K.L.; Conway, T.C.; Garg, S.; Queiroz, C.; Reumann, M.; Holt, K.; Rusu, L.I. WGS Analysis and Interpretation in Clinical and Public Health Microbiology Laboratories: What Are the Requirements and How Do Existing Tools Compare? Pathogens 2014, 3, 437-458. https://doi.org/10.3390/pathogens3020437

AMA Style

Wyres KL, Conway TC, Garg S, Queiroz C, Reumann M, Holt K, Rusu LI. WGS Analysis and Interpretation in Clinical and Public Health Microbiology Laboratories: What Are the Requirements and How Do Existing Tools Compare? Pathogens. 2014; 3(2):437-458. https://doi.org/10.3390/pathogens3020437

Chicago/Turabian Style

Wyres, Kelly L., Thomas C. Conway, Saurabh Garg, Carlos Queiroz, Matthias Reumann, Kathryn Holt, and Laura I. Rusu 2014. "WGS Analysis and Interpretation in Clinical and Public Health Microbiology Laboratories: What Are the Requirements and How Do Existing Tools Compare?" Pathogens 3, no. 2: 437-458. https://doi.org/10.3390/pathogens3020437

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