A Novel Jeilongvirus from Florida, USA, Has a Broad Host Cell Tropism Including Human and Non-Human Primate Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Virus Source
2.2. Preliminary Virus Isolation Attempt in Vero E6 Cells
2.3. Next-Generation Sequencing
2.4. Phylogenetic and Genetic Analyses
2.5. Real-Time RT-PCR Assay
2.6. Host Species Tropism Studies
2.7. Plaque Assays
3. Results
3.1. Initial Isolation of Virus in Vero E6 Cells
3.2. Sequencing Results
3.3. Phylogenetic Analyses
3.4. Host Cell Tropism and Virus Yields
3.5. rRT-PCR Assay
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Appendix A
Appendix B
References
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Oligonucleotide ID | Sequence (5′ to 3′) |
---|---|
RodparamyxF | CCT CCA TGG TTT GGA ACA CAT GCC |
RodparamyxP | 6-FAM-CCT GGG GGA GGG ATC AGG GGC AAT GC-BHQ-1 |
RodparamyxR | GCC TCA GAA GGG TAG ACA TGG TG |
Gene | Length (Ribonucleotides) | Genomic Positions | Gene Product | Amino Acid Length |
---|---|---|---|---|
Leader sequence | 93 | 1–3 | N/A a | N/A |
N | 1647 | 31–1677 | Nucleocapsid protein | 548 |
TIS b | 3 | 1774–1776 | N/A | N/A |
P | 1470 | 1863–3332 | Phosphoprotein | 489 |
P/V/C editing site | 16 | 2551–2566 | N/A | N/A |
V | 894 c | 1863–2755 | V protein | 298 |
C | 456 | 1906–2364 | C protein | 152 |
W | 924 d | 1863–2784 | W protein | 308 |
TIS | 3 | 3509–3511 | N/A | N/A |
M | 1017 | 3550–4566 | Matrix protein | 339 |
TIS | 3 | 4829–4831 | N/A | N/A |
F | 1641 | 4975–6615 | Fusion protein | 546 |
TIS | 3 | 6631–6633 | N/A | N/A |
tM | 777 | 6745–7521 | Transmembrane protein | 258 |
TIS | 3 | 7701–7703 | N/A | N/A |
RBP | 2352 | 7753–10,104 | Receptor-binding protein | 783 |
TIS | 3 | 10,290–10,292 | N/A | N/A |
L; RdRp | 6759 | 10,363–16,941 | Large; RNA-dependent RNA polymerase | 2192 |
Trailer sequence | 530 | 16,492–17,021 | N/A | N/A |
Cell Line | Virus Titer, Log10PFU/mL * |
---|---|
A549 | 5.3 ± 0.3 |
BHK-21 | 5.9 ± 0.4 |
Caco-2 | 5.3 ± 0.7 |
CV-1 | 6.8 ± 0.1 |
HeLa | 4.6 ± 0.7 |
LLC-MK2 | 7.1 ± 0.1 |
MDCK | 4.0 ± 0.3 |
MRC-5 | 6.4 ± 0.7 |
Neuro-2a | 3.5 ± 1.5 |
OHH1k | 2.9 ± 0.1 |
SAEC | 6.0 ± 0.6 |
Vero E6 | 7.2 ± 0.3 |
Tb1Lu | 2.6 ± 0.4 |
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DeRuyter, E.; Subramaniam, K.; Wisely, S.M.; Morris, J.G., Jr.; Lednicky, J.A. A Novel Jeilongvirus from Florida, USA, Has a Broad Host Cell Tropism Including Human and Non-Human Primate Cells. Pathogens 2024, 13, 831. https://doi.org/10.3390/pathogens13100831
DeRuyter E, Subramaniam K, Wisely SM, Morris JG Jr., Lednicky JA. A Novel Jeilongvirus from Florida, USA, Has a Broad Host Cell Tropism Including Human and Non-Human Primate Cells. Pathogens. 2024; 13(10):831. https://doi.org/10.3390/pathogens13100831
Chicago/Turabian StyleDeRuyter, Emily, Kuttichantran Subramaniam, Samantha M. Wisely, J. Glenn Morris, Jr., and John A. Lednicky. 2024. "A Novel Jeilongvirus from Florida, USA, Has a Broad Host Cell Tropism Including Human and Non-Human Primate Cells" Pathogens 13, no. 10: 831. https://doi.org/10.3390/pathogens13100831