Detection of Prions in Brain Homogenates and CSF Samples Using a Second-Generation RT-QuIC Assay: A Useful Tool for Retrospective Analysis of Archived Samples
Abstract
:1. Introduction
2. Results
2.1. Establishment of the Second-Generation RT-QuIC Assay
2.2. Analysis of Archived Brain Homogenates Using RT-QuIC
2.3. Analysis of Archived CSF Samples with the RT-QuIC Assay
3. Discussion
4. Materials and Methods
4.1. Ethics Statement
4.2. Prion Disease Samples
4.3. Production of Recombinant Prion Protein for the RT-QuIC Assay
4.4. Second-Generation RT-QuIC
4.5. CSF Protein Concentration Measurement
4.6. Data Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Group | Type | n (BH)/(CSF) | AST (Years ± sd) | Mean Age (Years) | t-tau (Pos./Tested) | 14-3-3 (Pos./Tested) |
---|---|---|---|---|---|---|
Sporadic TSE | MM1 | 3/3 | 2 ± 0 | 71 | 3/3 (100%) | 2/3 (66.7%) |
MM2 | 3/3 | 6.7 ± 0.6 | 65 | 1/1 (100%) | 2/3 (66.7%) | |
MV1 | 3/3 | 3.3 ± 0.6 | 63 | 2/2 (100%) | 1/2 (50%) | |
MV2 | 3/3 | 4.3 ± 2.5 | 70 | 3/3 (100%) | 2/3 (66.7%) | |
VV1 | 3/3 | 2 ± 0.8 | 64 | 3/3 (100%) | 2/3 (66.7%) | |
VV2 | 3/3 | 6.3 ± 3.6 | 64 | 2/2 (100%) | 2/2 (100%) | |
VPSPr (VV) | 1/0 | 2 ± 0 | 73 | NT | NT | |
Genetic TSE | E200K | 15/3 | 9 ± 3.5 | 61 | 7/8 (87.5%) | 6/12 (50%) |
GSS | 3/3 | 8 ± 3 | 48 | 2/2 (100%) | 0/2 (0%) | |
D178N (MV) | 1/0 | 13 ± 0 | 59 | NT | 1/1 (100%) | |
R208H (VV) | 1/0 | 10 ± 0 | 62 | 1/1 (100%) | 1/1 (100%) | |
OND | FTLD-tau | 5/5 | 3.2 ± 2.2 | 71 | 0/3 (0%) | 0/3 (0%) |
FTLD-TDP | 5/5 | 3.6 ± 1.7 | 64 | 0/2 (0%) | 0/3 (0%) | |
AD | 5/5 | 4.6 ± 2.3 | 70 | NT | 1/2 (50%) | |
AD + others | 4/4 | 2 ± 0 | 79 | 1/1 (100%) | NT | |
Huntington dis. | 4/5 | 5.8 ± 2.2 | 72 | 0/1 (0%) | NT | |
PD | 5/5 | 6 ± 2.9 | 70 | 1/1 (100%) | NT | |
Healthy corneal donors | - | 25/0 | - | - | NT | NT |
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Moško, T.; Galušková, S.; Matěj, R.; Brůžová, M.; Holada, K. Detection of Prions in Brain Homogenates and CSF Samples Using a Second-Generation RT-QuIC Assay: A Useful Tool for Retrospective Analysis of Archived Samples. Pathogens 2021, 10, 750. https://doi.org/10.3390/pathogens10060750
Moško T, Galušková S, Matěj R, Brůžová M, Holada K. Detection of Prions in Brain Homogenates and CSF Samples Using a Second-Generation RT-QuIC Assay: A Useful Tool for Retrospective Analysis of Archived Samples. Pathogens. 2021; 10(6):750. https://doi.org/10.3390/pathogens10060750
Chicago/Turabian StyleMoško, Tibor, Soňa Galušková, Radoslav Matěj, Magdalena Brůžová, and Karel Holada. 2021. "Detection of Prions in Brain Homogenates and CSF Samples Using a Second-Generation RT-QuIC Assay: A Useful Tool for Retrospective Analysis of Archived Samples" Pathogens 10, no. 6: 750. https://doi.org/10.3390/pathogens10060750
APA StyleMoško, T., Galušková, S., Matěj, R., Brůžová, M., & Holada, K. (2021). Detection of Prions in Brain Homogenates and CSF Samples Using a Second-Generation RT-QuIC Assay: A Useful Tool for Retrospective Analysis of Archived Samples. Pathogens, 10(6), 750. https://doi.org/10.3390/pathogens10060750