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17 December 2025

The Performance of Protein Meal from Hermetia illucens Larvae in Hetero-Clarias Hybrid Farming

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1
Department of Zoology and Ecology, Faculty of Animal Science and Biotechnologies, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400372 Cluj-Napoca, Romania
2
Endocrinology Clinic, Cluj County Emergency Clinical Hospital, 400347 Cluj-Napoca, Romania
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Department of Endocrinology, Faculty of Medicine, “Iuliu Haţieganu” University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania
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Department of Aquaculture, Faculty of Animal Science and Biotechnologies, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400372 Cluj-Napoca, Romania
This article belongs to the Special Issue Insects as the Nutrition Source in Animal Feed

Simple Summary

In this study, we tested whether fishmeal, a commonly used ingredient in aquafeeds, can be partly replaced with black soldier fly (Hermetia illucens) larvae meal. We have also checked if adding a commercial enzyme complex (Hostazyme X) would help fish absorb this insect-based feed better. The experiment involved 240 hybrid catfish (hetero-clarias) fed different diets for 80 days: some with only fishmeal, and others where 40%, 50%, or 60% of the fishmeal was replaced with larvae meal, with or without enzymes. The fish fed the insect-based diets generally showed better growth, and their blood parameter values, while sometimes different, remained within healthy limits. The best results came from the diet where 40% of fishmeal was replaced with larvae meal and combined with the enzyme supplement. This suggests that insect meal, especially with enzymes, can successfully replace part of fishmeal in the aquaculture of hetero-clarias.

Abstract

In the context of healthy and sustainable alternatives to fishmeal, insect meal asserts itself as a potentially healthy ingredient in aquafeeds. The aim of this study was to determine the possibility of successfully replacing fishmeal with Hermetia illucens larvae meal in the diet of the hetero-clarias hybrid, and to improve the overall bioavailability of the meal by supplementation with an enzyme complex (Hostazyme X). There were eight treatments: 0, 40, 50, and 60% replacement of fishmeal with larvae meal in the diet, with and without the enzyme supplement. In total, 240 fish with a mean weight of 11.43 ± 0.32 g were employed in the treatments for 80 days. Growth parameters (specific growth rate, feed conversion ratio, daily feed intake, daily weight gain, percentage weight gain, survival rate, production index, metabolic grow rate), hematological parameters and blood serum biochemistry were determined and analyzed. There was an improvement (p < 0.05) in most growth parameters for fish fed experimental diets. Blood parameters, although significantly different (p < 0.05) in some cases, were within a normal range for fish physiology. Thus, the partial replacement (40%) of fishmeal with larvae meal and supplementation with an enzyme complex produced the best growth performance compared to other treatments and controls.

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