Next Article in Journal
Transcriptomic and Metabolomic Responses of Rice Plants to Cnaphalocrocis medinalis Caterpillar Infestation
Next Article in Special Issue
Exploring Simplified Methods for Insect Chitin Extraction and Application as a Potential Alternative Bioethanol Resource
Previous Article in Journal
Evaluating Response of Mass-Reared and Irradiated Navel Orangeworm, Amyelois transitella (Lepidoptera: Pyralidae), to Crude Female Pheromone Extract
Previous Article in Special Issue
Transcriptome Analysis of Psacothea hilaris: De Novo Assembly and Antimicrobial Peptide Prediction
 
 
Article

Antimicrobial Activity of an Extract of Hermetia illucens Larvae Immunized with Lactobacillus casei against Salmonella Species

by 1,†, 2,† and 3,*
1
Department of Pharmacology, College of Medicine, Dongguk University, Gyeongju 38766, Korea
2
Department of Integrative Bio-Industrial Engineering, Sejong University, Seoul 05006, Korea
3
Department of Biochemistry, College of Medicine, Dongguk University, Gyeongju 38766, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Insects 2020, 11(10), 704; https://doi.org/10.3390/insects11100704
Received: 15 September 2020 / Revised: 8 October 2020 / Accepted: 13 October 2020 / Published: 15 October 2020
(This article belongs to the Special Issue Insects and Their Derivatives for Human Practical Uses)
In this investigation, we found antimicrobial activity of the extract of Lactobacillus casei–infected Hermetia illucens larvae (HIL) against Salmonella species. The result demonstrates that the extract is a powerful natural antibiotic and preservative.
The expressions of antimicrobial peptides (AMPs) in the larvae of the black soldier fly, Hermetia illucens, were significantly increased by pathogen or stimulant induced innate immunity activation. We immunized H. illucens fifth instar larvae with five different Lactobacillus species, that is, Lactobacillus acidophilus, L. brevis, L. casei, L. fermentum, or L. delbrueckii, to induce the mass production of AMPs and selected optimal immune inducers. Antimicrobial activities in hemolymph and H. illucens larvae (HIL) extract were evaluated against three salmonella species (Salmonella pullorum, Salmonella typhimurium, and Salmonella enteritidis). Highest antimicrobial activity was shown by the hemolymph of HIL immunized by L. casei and its activity was closely linked with the inductions of cecropin 1 (HiCec1) and defensin 1 (HiDef1) gene expressions. Furthermore, antimicrobial activity in hemolymph was stable to heat and pH and the growth of three Salmonella species were dramatically suppressed by HIL hemolymph and extract after immunization with L. casei. The minimal inhibitory concentration (MICs) of L. casei-immunized HIL extract against Staphylococcus aureus, Escherichia coli, and Salmonella species ranged from 100~200 µg/100 µL and no cytotoxicity to CaCo-2 and L929 cells were observed in the concentration range 100~40,000 µg/100 µL. Taken together, the present investigation demonstrates that L. casei-immunized HIL extract is a powerful natural antibiotic and preservative that can prevent contamination by Salmonella species. View Full-Text
Keywords: antimicrobial activity; natural antibiotics; antimicrobial peptide; Hermetia illucens; Salmonella antimicrobial activity; natural antibiotics; antimicrobial peptide; Hermetia illucens; Salmonella
Show Figures

Figure 1

MDPI and ACS Style

Lee, K.-S.; Yun, E.-Y.; Goo, T.-W. Antimicrobial Activity of an Extract of Hermetia illucens Larvae Immunized with Lactobacillus casei against Salmonella Species. Insects 2020, 11, 704. https://doi.org/10.3390/insects11100704

AMA Style

Lee K-S, Yun E-Y, Goo T-W. Antimicrobial Activity of an Extract of Hermetia illucens Larvae Immunized with Lactobacillus casei against Salmonella Species. Insects. 2020; 11(10):704. https://doi.org/10.3390/insects11100704

Chicago/Turabian Style

Lee, Kyu-Shik, Eun-Young Yun, and Tae-Won Goo. 2020. "Antimicrobial Activity of an Extract of Hermetia illucens Larvae Immunized with Lactobacillus casei against Salmonella Species" Insects 11, no. 10: 704. https://doi.org/10.3390/insects11100704

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop