Coenzyme Q10 and Parkinsonian Syndromes: A Systematic Review

Félix Javier Jiménez-Jiménez; Hortensia Alonso-Navarro; Elena García-Martín; José A. G. Agúndez

doi:10.3390/jpm12060975

,

and

¹

Section of Neurology, Hospital Universitario del Sureste, Ronda del Sur 10, E28500 Arganda del Rey, Spain

²

ARADyAL Instituto de Salud Carlos III, University Institute of Molecular Pathology Biomarkers, Universidad de Extremadura, E10071 Cáceres, Spain

^*

Author to whom correspondence should be addressed.

J. Pers. Med.2022, 12(6), 975;https://doi.org/10.3390/jpm12060975

Version Notes

Order Reprints

Abstract

Coenzyme Q₁₀ (CoQ₁₀) has an important role as an antioxidant. Being that oxidative stress is one of the mechanisms involved in the pathogenesis of Parkinson’s disease (PD) and other neurodegenerative diseases, several studies addressed the concentrations of CoQ₁₀ in the different tissues of patients with PD and other parkinsonian syndromes (PS), trying to elucidate their value as a marker of these diseases. Other studies addressed the potential therapeutic role of CoQ₁₀ in PD and PS. We underwent a systematic review and a meta-analysis of studies measuring tissue CoQ₁₀ concentrations which shows that, compared with controls, PD patients have decreased CoQ₁₀ levels in the cerebellar cortex, platelets, and lymphocytes, increased total and oxidized CoQ₁₀ levels in the cerebrospinal fluid and a non-significant trend toward decreased serum/plasma CoQ₁₀ levels. Patients with multiple system atrophy (MSA) showed decreased CoQ₁₀ levels in the cerebellar cortex, serum/plasma, cerebrospinal fluid, and skin fibroblasts. Patients with Lewy body dementia (LBD) showed decreased cerebellar cortex CoQ₁₀, and those with progressive supranuclear palsy (PSP) had decreased CoQ₁₀ levels in the cerebrospinal fluid. A previous meta-analysis of studies addressing the therapeutic effects of CoQ₁₀ in PD showed a lack of improvement in patients with early PD. Results of the treatment with CoQ₁₀ in PSP should be considered preliminary. The potential role of CoQ₁₀ therapy in the MSA and selected groups of PD patients deserves future studies.

Keywords:

coenzyme Q₁₀; tissue concentrations; therapeutics; Parkinson’s disease; multiple system atrophy; progressive supranuclear palsy; Lewy body dementia

1. Introduction

Coenzyme Q₁₀ (CoQ₁₀, Figure 1), which is also known as ubiquinone, is a 1,4-benzoquinone that is present in the majority of tissues in the human body. It is an important component of the electron transport chain in the mitochondria, participating in the generation of cellular energy through oxidative phosphorylation. In tissues, CoQ₁₀ can be present in three redox states: fully oxidized (ubiquinone), partially oxidized (semiquinone or ubisemiquinone), and fully reduced (ubiquinol). Together with mitochondria, CoQ₁₀ is present in the endoplasmic reticulum, Golgi apparatus, lysosomes, and peroxisomes. CoQ₁₀ has important antioxidant actions (both by scavenging free radicals and by the regeneration of other antioxidants, such as alpha-tocopherol or ascorbate acid), giving protection to cells against oxidative stress processes [1,2].

Figure 1. Chemical structure of coenzyme Q₁₀.

Because oxidative stress is one of the most important pathogenetic mechanisms of Parkinson’s disease (PD) and other neurodegenerative disorders [3,4], and because of the role of CoQ₁₀ as an antioxidant, both the study of CoQ₁₀ concentrations in different tissues of patients diagnosed with PD and/or other parkinsonian syndromes and the potential therapeutic role of CoQ₁₀ in these diseases, have been the matter of several publications over the last two decades. The aim of this systematic review and meta-analysis is to analyze the results of studies addressing the tissular concentrations of CoQ₁₀ in patients diagnosed with parkinsonian syndromes compared to healthy controls and the results of therapeutic trials of CoQ₁₀ in PD and other causes of parkinsonism.

2. Methods

2.1. Search Strategy and Criteria for Eligibility of Studies

A literature search using several well-known databases (PubMed, EMBASE, Web of Science (WOS) Main Collection) from 1966 until 4 May 2022, was performed. The term “coenzyme Q₁₀” was crossed with “Parkinson’s disease” (356, 924, and 303 items were found in PubMed, EMBASE, and WOS, respectively), “parkinsonism” (403, 183, and 39 items were found in PubMed, EMBASE, and WOS, respectively), parkinsonian syndromes (223, 7, and 6 items were found in PubMed, EMBASE, and WOS, respectively), “multiple system atrophy” (36, 125, and 56 items were found in PubMed, EMBASE, and WOS, respectively), “Lewy body dementia” (8, 39, and 10 items were found in PubMed, EMBASE, and WOS, respectively), “Lewy body disease” (8, 62, and 24 items were found in PubMed, EMBASE, and WOS, respectively), “progressive supranuclear palsy” (31, 66, and 9 items were found in PubMed, EMBASE, and WOS, respectively), and “corticobasal degeneration” (4, 28, and 5 items were found in PubMed, EMBASE, and WOS, respectively). A total of 1054 references were retrieved by the whole search and examined one by one, and then those that were strictly related to the proposed topics, without language restrictions, were selected, excluding the duplicated articles and abstracts. The flowcharts for the selection of eligible studies—following the PRISMA guidelines [5]—analyzing tissue CoQ₁₀ concentrations in patients with several types of parkinsonian syndrome and controls, and therapeutic trials with CoQ₁₀ in parkinsonian syndromes, are plotted in Figure 2.

Figure 2. PRISMA Flowchart for the studies assessing tissue concentrations of coenzyme Q10 in parkinsonian syndromes, and for therapeutic trials with CoQ10 in parkinsonian syndromes.

2.2. Selection of Studies and Methodology for the Meta-Analyses

Meta-analyses of those observational eligible studies that assessed the concentrations of CoQ₁₀ in tissues were performed. The first author, year of publication, country, study design, and quantitative measures were extracted, and the risk of bias was analyzed by using the Newcastle–Ottawa Scale [6]. Data from selected studies analyzing the tissular concentrations of CoQ₁₀ in patients diagnosed with PD compared to controls, patients diagnosed with multiple system atrophy (MSA) compared to controls, and patients with Lewy body dementia (LBD), progressive supranuclear palsy (PSP), and cortical basal degeneration (CBD) compared to healthy controls are summarized, respectively, in Table 1, Table 2 and Table 3. The plasma/serum and CSF levels of coenzyme Q10 were converted to nmol/mL, and brain tissue levels to pmol/mL, when necessary. The meta-analyses followed the PRISMA [5] (Table S1) and MOOSE guidelines [7] (Table S2) and were carried out by using the R software package meta [8]. We applied the random-effects model because of the high heterogeneity across studies, and we used the inverse variance method for the meta-analytical procedure, the DerSimonian-Laird as an estimator for Tau², the Jackson method for the confidence interval of Tau² and Tau, and the Hedges’ g (bias-corrected standardized mean difference). We calculated the statistical power to detect differences in mean values (alpha = 0.05) for the pooled samples when stated in the text.

Table 1. Coenzyme Q10 Concentrations in Several Tissues from Parkinson’s Disease (PD) Patients and Healthy Controls (HC).

Table 2. Coenzyme Q10 Concentrations in Several Tissues from Patients with Multisystem Atrophy (MSA) and Healthy Controls (HC).

Table 3. Coenzyme Q10 Concentrations in Several Tissues from Patients with Lewy Body Dementia (LBD), Progressive Supranuclear Palsy, and Cortical Basal Degeneration Compared with Healthy Controls (HC).

3. Results

3.1. Studies Assessing Tissular CoQ₁₀ Concentrations

3.1.1. Parkinson’s Disease

Serum/Plasma

Matsubara et al. [28] reported decreased serum CoQ₁₀ levels in PD patients. However, the comparison group was composed of patients with cerebral infarction instead of healthy controls. The pooled results of the seven studies assessing the serum or plasma total CoQ₁₀ levels in PD patients compared with controls [9,10,11,12,13,14,15], did not show significant differences in this value between the two groups (Table 1, Figure 3a), as was the case with the two studies assessing the serum or plasma CoQ₁₀ corrected to cholesterol levels (Table 1, Figure 3b) [9,14]. However, the serum/plasma oxidized CoQ₁₀/total CoQ₁₀ ratio was found to be significantly higher in PD patients compared with controls in two of these studies (Table 1, Figure 3c) [11,13]. Two studies showed a lack of differences in the serum/plasma oxidized CoQ₁₀ and in the reduced CoQ₁₀ concentrations between PD patients and controls [11,14], although there were substantial differences in these values between these studies.

Figure 3. Meta-analyses of studies assessing serum/plasma total CoQ₁₀ levels [7,8,9,10,11,12,13], serum/plasma CoQ₁₀ corrected to cholesterol levels (b) [7,12], and serum/plasma oxidized CoQ₁₀/total CoQ₁₀ ratio (c) in PD patients compared with controls [9,11].

Blood Cells

Two studies showed decreased CoQ₁₀ concentrations in platelets [16] and lymphocytes [17], respectively, from patients with PD compared with healthy controls (Table 1).

Cerebrospinal Fluid (CSF)

According to two studies, PD patients showed increased total [18,19] and oxidized [18] CSF CoQ₁₀ concentrations (Table 1).

Brain

The pooled data from three studies [20,21,22] showed decreased CoQ₁₀ levels in the cerebellar cortex of PD patients in comparison with controls (Table 1, Figure 4), while concentrations in the cerebral cortex [20,21], striatum [20], and substantia nigra [20] did not differ significantly between the two groups (Table 1).

Figure 4. Meta-analyses of studies assessing CoQ₁₀ concentrations in the cerebellar cortex of PD patients and controls [18,19,20].

Skin Fibroblasts

Del Hoyo et al. [23] reported similar CoQ₁₀ concentrations in skin fibroblasts from PD patients and controls (Table 1).

The pooled data from the three studies, addressing serum/plasma total CoQ₁₀ concentrations [14,15,24], showed a decrease in this value in patients with MSA compared with controls (Table 2, Figure 5). There have been reported decreased CoQ₁₀ concentrations in the CSF [19], cerebellum cortex [21,22], and skin fibroblasts [25] from MSA patients (Table 2), although the pooled data from studies assessing cerebellum cortex CoQ₁₀ levels did not reach statistical significance. In contrast, the cerebral cortex [21,22] and striatum [21] CoQ₁₀ levels were similar in MSA and controls (Table 2).

Figure 5. Meta-analyses of studies assessing serum/plasma total CoQ₁₀ levels, serum/plasma total CoQ₁₀ concentrations in MSA patients and controls [12,13,22].

3.1.2. Other Parkinsonian Syndromes

The results of studies addressing CoQ₁₀ concentrations in patients with other parkinsonian syndromes, compared with healthy controls, are summarized in Table 3. In summary, patients with Lewy body dementia (DLB) showed similar CoQ₁₀ concentrations to those of the control in serum/plasma [26,27], and in the cerebral cortex [21], but lower CoQ₁₀ concentrations in the cerebellum cortex [21]. Patients diagnosed with progressive supranuclear palsy (PSP) showed decreased CSF CoQ₁₀ levels [19], and patients with cortical basal degeneration showed normal cerebral cortex CoQ₁₀ levels [21].

3.2. Studies Assessing Therapeutic Response to CoQ₁₀ Administration

3.2.1. Parkinson’s Disease

The results of the 10 eligible studies addressing the therapeutic response of CoQ₁₀ administration in patients with PD [29,30,31,32,33,34,35,36,37,38] are summarized in Table 4. One of these studies used an open-label design [29], while the others were randomized clinical placebo-controlled trials [30]. CoQ₁₀ was generally well-tolerated, according to the four studies assessing adverse effects [32,33,34,35,36]. Despite five of these studies showing a mild improvement in motor scales in PD patients [30,31,35,36,38], three meta-analyses [39,40,41], one of them including eight randomized clinical trials [41], concluded that CoQ₁₀ was not superior to the placebo in improving motor symptoms.

Table 4. Studies describing the effects of levodopa and dopamine agonists in patients with RBD.

The study by Yoritaka et al. [38] showed a significant improvement in motor symptoms of PD patients suffering from the “wearing-off” phenomenon, and Li et al. [37] described a positive effect of concomitant CoQ₁₀ and creatine therapy on cognitive impairment, assessed by the Montreal Cognitive Assessment (MoCA). However, these results are based on a small size series.

Mitsui et al. [42] reported the effects of the treatment with CoQ₁₀ 1200 mg/day in a patient diagnosed with familial MSA, in an advanced stage, related to the compound heterozygous nonsense (R387X) and missense (V393A) mutations in the COQ2 gene. The administration of CoQ₁₀ resulted in increased serum and CSF total CoQ₁₀ concentrations, the increased cerebral metabolic ratio of the oxygen measured by ¹⁵O₂ positron emission tomography (PET), and led to stability in several clinical scores (Barthel Index, Scale for the Assessment and Rating of Ataxia—SARA, International Cooperative Ataxia Rating Scale—ICARS, and the Unified Multiple System Atrophy Rating Scale—UMSARS) during 3 years of follow-up.

3.2.2. Progressive Supranuclear Palsy

Two randomized clinical trials studied the effects of CoQ₁₀ in patients diagnosed with PSP. Stamelou et al. [43], in a 6-week, monocenter, double-blind, randomized, placebo-controlled, phase II trial, including 21 clinically probable PSP patients assigned to a liquid nanodispersion of CoQ10 (doses of 5 mg/kg/day) or placebo, showed a mild improvement in a Frontal Assessment Battery and in the total scores of the PSP rating scale (PSPRS) in those assigned to CoQ₁₀, while there were no significant changes in the UPDRS and the Mini-Mental State Examination (MMSE). They did not describe the relevant adverse effects. As should be expected, plasma levels of CoQ10 increased in the treated, but not untreated patients. In patients receiving CoQ10 compared to those receiving the placebo, the ratio of high-energy phosphates to low-energy phosphates (adenosine-triphosphate to adenosine-diphosphate, and phosphocreatine to unphosphorylated creatine) increased significantly in the occipital lobe and showed a consistent trend towards an increase in the basal ganglia. For this reason, the authors suggested a possible disease-modifying neuroprotective of CoQ10.

In contrast, Apetauerova et al. [44], in a one-year, investigator-initiated, multicenter, randomized, placebo-controlled, double-blind clinical trial involving 61 patients diagnosed with PSP assigned to CoQ10 (2400 mg/day) or a placebo, found no significant differences between the two study groups in PSPRS (although there was a non-significant trend toward a slower decline in the CoQ₁₀ group), UPDRS, activities of daily living (ADL), MMSE, the 39-item Parkinson’s Disease Questionnaire (PDQ-39), and the 36-item Short-Form Health Survey (SF-36). Despite CoQ₁₀ being well-tolerated, 41% of participants withdrew from the study for different reasons.

4. Discussion and Conclusions

The possible role of CoQ₁₀ in the pathogenesis, or its value as a diagnostic marker of PD and other parkinsonian syndromes, has not been definitively established. In the case of PD, the pooled analyses of studies measuring CoQ₁₀ concentrations in the brain tissues [20,21,22], showed a significant decrease in the cerebellum cortex of PD patients (Table 1, Figure 4), which was likely related to the concentrations found in the larger control group of one of these studies [21], while CoQ₁₀ concentrations in the striatum, substantia nigra, and cerebral cortex were similar in PD patients and controls. Studies in platelets [16] and lymphocytes [17] showed a consistent decrease in CoQ₁₀ concentrations, while in the CSF, both the total [18,19] and oxidized CoQ₁₀ levels [18] were found to be increased in PD patients. The pooled data of the seven studies assessing serum/plasma CoQ₁₀ levels [9,10,11,12,13,14,15] showed a non-significant trend toward lower concentrations in PD patients compared with controls (Table 1, Figure 3a), while the percentage of oxidized vs. total CoQ₁₀ was increased in PD (Table 1). One study showed a surprisingly very high percentage of oxidized CoQ₁₀, which was related to the easy oxidation of the reduced to oxidized CoQ₁₀ from the moment of sample extraction because precautions were not taken to prevent this oxidation [14]. Finally, CoQ₁₀ levels in the skin fibroblasts were similar in PD patients and controls [23].

In MSA, CoQ₁₀ concentrations were found to be decreased in the cerebellar cortex in two studies (19, 20)—although the results of the pooled data did not reach statistical significance (Table 2)—in the serum/plasma [14,15,24], CSF [19], and skin fibroblasts [25]. Patients with LBD showed decreased cerebellar CoQ₁₀ [21] and patients with PSP showed decreased CoQ₁₀ concentrations [19] in single studies.

Due to their antioxidant actions, it was proposed that CoQ₁₀ administration could be a potential protective therapy in PD and other neurodegenerative diseases [45,46]. Moreover, the administration of CoQ₁₀ has shown neuroprotective effects in several models of experimental parkinsonism:

(a): CoQ₁₀ or idebenone (an analog of CoQ₁₀) attenuates the loss of striatal dopamine and dopaminergic axons, induced by 1-methyl-4-phenyl-1,2,5,6-tetrahydropyridine (MPTP) administration in rodents [47,48,49,50] and in monkeys [51].
(b): In rats, both the coadministration of CoQ₁₀ and creatine [52] or CoQ₁₀ and nicotinamide [53] have shown additive neuroprotective effects against striatal dopamine depletion after MPTP administration.
(c): In rats injected with 6-hydroxydopamine (6-OHDA), the coadministration of CoQ₁₀ and a mir-149sp mimic [54], or of CoQ₁₀ and bone marrow stromal cells (BMSC) [55], improves motor symptoms and prevents dopaminergic damage.
(d): CoQ₁₀ administration was also able to prevent iron-induced apoptosis in cultured human dopaminergic (SK-N-SH) neurons, in metallothionein gene-manipulated mice, and in alpha-synuclein knockout (alpha-synko) mice [56].
(e): CoQ₁₀ administration can prevent neurodegeneration and behavioral deterioration in rodents exposed to several toxins causing experimental parkinsonism, such as the pesticides paraquat [57,58], dichlorvos [59], and rotenone [60,61], and showed neuroprotective effects against rotenone in primary rat mesencephalic cultures [62] and human neuroblastoma cells [63]. Interestingly, the exposure of human neuroblastoma SH-SY5Y cells to commonly used organophosphate compounds, such as dichlorvos, methyl-parathion (parathion), and chlorpyrifos (CPF), induces an important decrease in CoQ₁₀ levels and complex II + III activity—both related to a decrease in neuronal cell viability. In this model, CoQ₁₀ supplementation can modestly although significantly increase complex II + III activity [64].
(f): CoQ₁₀ supplementation (with or without the concomitant treatment of levodopa) has shown a protective effect against chlorpromazine-induced parkinsonism in mice, including a reduction in mortality and catalepsy, an increase in dopamine levels, and a decrease in oxidative stress [65]. Similarly, CoQ₁₀ improved the forced swimming test, locomotor activity test, catalepsy, muscle coordination, and akinesia test, and reduced the dopamine depletion in haloperidol-induced parkinsonism in rats [66].

However, CoQ₁₀ had not shown neuroprotective effects in a Drosophila DJ-1 model of PD [67]. Moreover, idebenone can induce apoptotic death cells in human neuroblastoma cells [68]. On the other hand, MPTP and its metabolite 1-methyl-4-phenyl-2,3-dihydropyridinium (MPDP⁺) are also able to induce a reduction in CoQ₁₀, and a reduction in CoQ₁₀ promotes the conversion of MPDP⁺ to the active neurotoxin 1-methyl-4-phenylpiridinium (MPP+) and increases its neurotoxicity [69].

Several studies analyzed the effects of CoQ₁₀ administration on serum/plasma and CSF CoQ₁₀ levels. Lönnrot et al. [70] described a significant increase in the plasma CoQ₁₀ concentrations, but a lack of changes in the CSF CoQ₁₀ concentrations in five healthy individuals after oral supplementation with ascorbic acid and CoQ_10. Shults et al. [71], described an increase in plasma CoQ₁₀ concentrations in 17 subjects after the administration of an escalating dosage of coenzyme Q10 (1200, 1800, 2400, and 3000 mg/day) with a stable dosage of vitamin E (alpha-tocopherol) 1200 IU/day, reaching the maximum plasma concentration with 2400 mg/day. Nukui et al. [72] reported, both in a double-blind, placebo-controlled study involving 46 healthy volunteers humans and in an acute, single-dose administration study in rats, that the administration of a water-soluble type of CoQ₁₀ reached considerably higher serum CoQ₁₀ concentrations than conventional CoQ₁₀.

Despite the possible beneficial effects of CoQ₁₀ administration, its good absorption, the lack of important adverse effects, and the improvement in PD symptoms suggested by several studies [30,31,35,36,38], data from meta-analyses of randomized clinical trials did not suggest the general usefulness of this therapy in patients with PD [39,40,41]. Several biochemical studies suggest the presence of CoQ₁₀ deficiency in MSA, but the possible role of this compound in the treatment of MSA has not been explored yet. Although short-term use of CoQ₁₀ treatment in PSP showed promising effects [43], the results of a randomized clinical trial involving a small series of patients showed no beneficial effects [44].

Despite all these data, the improvement in motor symptoms reported in a small series of patients with PD and the “wearing-off” phenomenon under CoQ₁₀ therapy [38], and the improvement in cognitive impairment in patients treated with the combination of CoQ₁₀ and creatine [37] suggest that CoQ₁₀ could be useful in selected patients, and the role of personalized medicine could be important. In this regard, Seet et al. [73], in a preliminary study involving 16 PD patients treated with different doses of CoQ₁₀, described that patients who experienced a significant short-term reduction in the UPDRS score had lower baseline plasma ubiquinol and decreased F2-isoprostanes (CoQ₁₀ and F2-isoprostanes increased significantly at a 2400 mg/day dosage of CoQ₁₀), suggesting that the therapeutic response should depend on the baseline levels of these two compounds.

Moreover, a recent double-blind randomized, phase II, placebo-controlled study using an omics-based strategy with CoQ₁₀ has been recently proposed [74]. In this study, the assignation to a treatment group should be done after the stratification by the so-called “mitochondrial risk burden” in homozygous or compound heterozygous Parkin/PINK1 mutation carriers (P++), heterozygous Parkin/PINK1 mutation carriers (P+), and “omics” positive (omics+) and “omics” negative PD patients (omics-), those being omics+ with the highest and those who are omics- with the lowest cumulative burden of common genetic variants in genes that are related to mitochondrial function. Changes in the motor subscore of UPDRS should be the primary endpoint, and the appearance of motor fluctuations and non-motor symptoms in the ³¹P-magnetic resonance spectroscopy (³¹P-MRS) imaging results, and changes in structural and functional brain anatomy (MRI), should be the secondary endpoints.med-con

In summary, according to the current data, the possible value of the treatment with CoQ₁₀ in parkinsonian syndromes could deserve further studies, at least in selected subgroups of patients with PD and in patients diagnosed with MSA and PSP.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/jpm12060975/s1. Table S1. PRISMA Checklist. Table S2. MOOSE Checklist.

Author Contributions

F.J.J.-J.: Conceptualization, Methodology, Investigation, Validation, Formal analysis, Writing—original draft, Writing—review and editing, Project administration. H.A.-N.: Conceptualization, Methodology, Investigation, Validation, Formal analysis, Writing—original draft, Writing—review and editing, Project administration. E.G.-M.: Conceptualization, Methodology, Investigation, Validation, Formal analysis, Writing—original draft, Writing—review and editing, Project administration, Obtaining funding. J.A.G.A.: Conceptualization, Methodology, Investigation, Validation, Formal analysis, Writing—original draft, Writing—review and editing, Project administration, Obtaining funding. All authors have read and agreed to the published version of the manuscript.

Funding

The work at the authors’ laboratory is supported in part by Grants RETICS RD16/0006/0004 (ARADyAL), PI18/00540 and PI21/01683 from Fondo de Investigación Sanitaria, Instituto de Salud Carlos III, Madrid, Spain and IB20134 and GR21073 from Junta de Extremadura, Mérida, Spain. This study was partially funded with FEDER funds.

Institutional Review Board Statement

Not applicable because this is a review article.

Informed Consent Statement

Not applicable because this is a review article.

Data Availability Statement

All data related to the current study, intended for reasonable use, is available from J.A.G. Agúndez (University Institute of Molecular Pathology Biomarkers, University of Extremadura -UNEx ARADyAL Instituto de Salud Carlos III, Av/de la Universidad S/N, E10071 Cáceres. Spain) and F.J. Jiménez-Jiménez (Section of Neurology, Hospital del Sureste, Arganda del Rey, Madrid, Spain).

Acknowledgments

We recognize the efforts of the personnel at the Library of Hospital Universitario del Sureste, Arganda del Rey, who retrieved an important number of papers for us.

Conflicts of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

References

Crane, F.L. Biochemical functions of coenzyme Q10. J. Am. Coll. Nutr. 2001, 20, 591–598. [Google Scholar] [CrossRef] [PubMed]
Mantle, D.; Heaton, R.A.; Hargreaves, I.P. Coenzyme Q10, Ageing and the Nervous System, An Overview. Antioxidants 2021, 11, 2. [Google Scholar] [CrossRef] [PubMed]
Alonso-Navarro, H.; Jiménez-Jiménez, F.J.; Pilo de la Fuente, B.; Plaza-Nieto, J.F. Mecanismos patogénicos de la enfermedad de Parkinson. In Tratado de los Trastornos del Movimiento, 2nd ed.; Jiménez-Jiménez, F.J., Luquin, M.R., Molina, J.A., Linazasoro, G., Eds.; Viguera Editores: Barcelona, Spain, 2008; Volume 1, pp. 425–485. [Google Scholar]
Jurcau, A. Insights into the Pathogenesis of Neurodegenerative Diseases, Focus on Mitochondrial Dysfunction and Oxidative Stress. Int. J. Mol. Sci. 2021, 22, 11847. [Google Scholar] [CrossRef] [PubMed]
Moher, D.; Liberati, A.; Tetzlaff, J.; Altman, D.G.; Group, P. Preferred reporting items for systematic reviews and meta-analyses, the PRISMA statement. PLoS Med. 2009, 6, e1000097. [Google Scholar] [CrossRef] [Green Version]
Wells, G.A.; Shea, B.; O’Connell, D.; Peterson, J.; Welch, V.; Losos, M.; Tugwell, P. The Newcastle-Ottawa Scale (NOS) for assessing the quality if nonrandomized studies in meta analyses. Available online: http://www.ohri.ca/programs/clinical_epidemiology/oxford.asp (accessed on 10 May 2022).
Stroup, D.F.; Berlin, J.A.; Morton, S.C.; Olkin, I.; Williamson, G.D.; Rennie, D.; Moher, D.; Becker, B.J.; Sipe, T.A.; Thacker, S.B. Meta-analysis of observational studies in epidemiology, a proposal for reporting. Meta-analysis Of Observational Studies in Epidemiology (MOOSE) group. JAMA 2009, 283, 2008–2012. [Google Scholar] [CrossRef]
Balduzzi, S.; Rücker, G.; Schwarzer, G. How to perform a meta-analysis with R: A practical tutorial. Evid Based Ment Health. 2019, 22, 153–160. [Google Scholar] [CrossRef] [Green Version]
Jiménez-Jiménez, F.J.; Molina, J.A.; de Bustos, F.; García-Redondo, A.; Gómez-Escalonilla, C.; Martínez-Salio, A.; Berbel, A.; Camacho, A.; Zurdo, M.; Barcenilla, B.; et al. Serum levels of coenzyme Q10 in patients with Parkinson’s disease. J. Neural. Transm. 2000, 107, 177–181. [Google Scholar] [CrossRef]
Buhmann, C.; Arlt, S.; Kontush, A.; Möller-Bertram, T.; Sperber, S.; Oechsner, M.; Stuerenburg, H.J.; Beisiegel, U. Plasma and CSF markers of oxidative stress are increased in Parkinson’s disease and influenced by antiparkinsonian medication. Neurobiol. Dis. 2004, 15, 160–170. [Google Scholar] [CrossRef]
Sohmiya, M.; Tanaka, M.; Tak, N.W.; Yanagisawa, M.; Tanino, Y.; Suzuki, Y.; Okamoto, K.; Yamamoto, Y. Redox status of plasma coenzyme Q10 indicates elevated systemic oxidative stress in Parkinson’s disease. J. Neurol. Sci. 2004, 223, 161–166. [Google Scholar] [CrossRef]
Bolner, A.; Micciolo, R.; Bosello, O.; Nordera, G.P. A Panel of Oxidative Stress Markers in Parkinson’s Disease. Clin. Lab. 2016, 62, 105–112. [Google Scholar] [CrossRef]
Gorgone, G.; Currò, M.; Ferlazzo, N.; Parisi, G.; Parnetti, L.; Belcastro, V.; Tambasco, N.; Rossi, A.; Pisani, F.; Calabresi, P.; et al. Coenzyme Q10, hyperhomocysteinemia and MTHFR C677T polymorphism in levodopa-treated Parkinson’s disease patients. Neuromolecular Med. 2012, 14, 84–90. [Google Scholar] [CrossRef]
Kasai, T.; Tokuda, T.; Ohmichi, T.; Ishii, R.; Tatebe, H.; Nakagawa, M.; Mizuno, T. Serum Levels of Coenzyme Q10 in Patients with Multiple System Atrophy. PLoS One 2016, 11, e0147574. [Google Scholar] [CrossRef]
Du, J.; Wang, T.; Huang, P.; Cui, S.; Gao, C.; Lin, Y.; Fu, R.; Shen, J.; He, Y.; Tan, Y.; et al. Clinical correlates of decreased plasma coenzyme Q10 levels in patients with multiple system atrophy. Parkinsonism Relat. Disord. 2018, 57, 58–62. [Google Scholar] [CrossRef]
Götz, M.E.; Gerstner, A.; Harth, R.; Dirr, A.; Janetzky, B.; Kuhn, W.; Riederer, P.; Gerlach, M. Altered redox state of platelet coenzyme Q10 in Parkinson’s disease. J. Neural. Transm. 2000, 107, 41–48. [Google Scholar]
Mischley, L.K.; Allen, J.; Bradley, R. Coenzyme Q10 deficiency in patients with Parkinson’s disease. J. Neurol. Sci. 2012, 318, 72–75. [Google Scholar] [CrossRef] [Green Version]
Isobe, C.; Murata, T.; Sato, C.; Terayama, Y. Increase of oxidized/total coenzyme Q-10 ratio in cerebrospinal fluid in patients with Parkinson’s disease. J. Clin. Neurosci. 2007, 14, 340–343. [Google Scholar] [CrossRef]
Compta, Y.; Giraldo, D.M.; Muñoz, E.; Antonelli, F.; Fernández, M.; Bravo, P.; Soto, M.; Cámara, A.; Torres, F.; Martí, M.J.; et al. Cerebrospinal fluid levels of coenzyme Q10 are reduced in multiple system atrophy. Parkinsonism Relat. Disord. 2018, 46, 16–23. [Google Scholar] [CrossRef]
Hargreaves, I.P.; Lane, A.; Sleiman, P.M. The coenzyme Q10 status of the brain regions of Parkinson’s disease patients. Neurosci Lett. 2008, 447, 17–19. [Google Scholar] [CrossRef]
Schottlaender, L.V.; Bettencourt, C.; Kiely, A.P.; Chalasani, A.; Neergheen, V.; Holton, J.L.; Hargreaves, I.; Houlden, H. Coenzyme Q10 Levels Are Decreased in the Cerebellum of Multiple-System Atrophy Patients. PLoS One 2016, 11, e0149557. [Google Scholar] [CrossRef] [Green Version]
Barca, E.; Kleiner, G.; Tang, G.; Ziosi, M.; Tadesse, S.; Masliah, E.; Louis, E.D.; Faust, P.; Kang, U.J.; Torres, J.; et al. Decreased Coenzyme Q10 Levels in Multiple System Atrophy Cerebellum. J. Neuropathol. Exp. Neurol. 2016, 75, 663–672. [Google Scholar] [CrossRef] [Green Version]
Del Hoyo, P.; García-Redondo, A.; de Bustos, F.; Molina, J.A.; Sabed, Y.; Alonso-Navarro, H.; Caballero, L.; Arenas, J.; Agúndez, J.A.; Jiménez-Jiménez, F.J. Oxidative stress in skin fibroblasts cultures from patients with Parkinson’s disease. BMC Neurol. 2010, 10, 95. [Google Scholar] [CrossRef] [Green Version]
Mitsui, J.; Matsukawa, T.; Yasuda, T.; Ishiura, H.; Tsuji, S. Plasma Coenzyme Q10 Levels in Patients With Multiple System Atrophy. JAMA Neurol. 2016, 73, 977–980. [Google Scholar] [CrossRef] [Green Version]
Compagnoni, M.C.; Kleiner, G.; Bordoni, A.; Fortunato, F.; Ronchi, D.; Salani, S.; Guida, M.; Corti, C.; Pichler, I.; Bergamini, C.; et al. Mitochondrial dysfunction in fibroblasts of Multiple System Atrophy. Biochim. Biophys. Acta Mol. Basis. Dis. 2018, 1864, 3588–3597. [Google Scholar] [CrossRef]
Molina, J.A.; de Bustos, F.; Ortiz, S.; Del Ser, T.; Seijo, M.; Benito-Léon, J.; Oliva, J.M.; Pérez, S.; Manzanares, J. Serum levels of coenzyme Q in patients with Lewy body disease. J. Neural. Transm. 2002, 109, 1195–1201. [Google Scholar] [CrossRef]
Gironi, M.; Bianchi, A.; Russo, A.; Alberoni, M.; Ceresa, L.; Angelini, A.; Cursano, C.; Mariano, E.; Nemni, R.; Kullmann, C.; et al. Oxidative imbalance in different neurodegenerative diseases with memory impairment. Neurodegener. Dis. 2011, 8, 129–137. [Google Scholar] [CrossRef]
Matsubara, T.; Azuma, T.; Yoshida, S.; Yamagami, T.; Okamoto, T.; Kishi, T. Serum coenzyme Q-10 level in Parkinson syndrome. In Biomedical and Clinical Aspects of Coenzyme Q; Folkers, K., Littarru, G.P., Yamagami, T., Eds.; Elsevier Science: Amsterdam, The Netherlands, 1991; pp. 159–166. [Google Scholar]
Strijks, E.; Kremer, H.P.; Horstink, M.W. Q10 therapy in patients with idiopathic Parkinson’s disease. Mol. Aspects Med. 1997, 18, S237–S240. [Google Scholar] [CrossRef]
Shults, C.; Oakes, D.; Kieburtz, K.; Beal, M.F.; Haas, R.; Plumb, S.; Juncos, J.L.; Nutt, J.; Shoulson, I.; Carter, J.; et al. Effects of coenzyme Q10 in early Parkinson disease, evidence of slowing of the functional decline. Arch. Neurol. 2002, 59, 1541–1550. [Google Scholar] [CrossRef]
Müller, T.; Büttner, T.; Gholipour, A.F.; Kuhn, W. Coenzyme Q10 supplementation provides mild symptomatic benefit in patients with Parkinson’s disease. Neurosci. Lett. 2003, 341, 201–204. [Google Scholar] [CrossRef]
NINDS NET-PD Investigators. A randomized clinical trial of coenzyme Q10 and GPI-1485 in early Parkinson disease. Neurology 2007, 68, 20–28. [Google Scholar]
Storch, A.; Jost, W.H.; Vieregge, P.; Spiegel, J.; Greulich, W.; Durner, J.; Müller, T.; Kupsch, A.; Henningsen, H.; Oertel, W.H.; et al. Randomized, double-blind, placebo-controlled trial on symptomatic effects of coenzyme Q(10) in Parkinson disease. Arch. Neurol. 2007, 64, 938–944. [Google Scholar] [CrossRef] [Green Version]
Parkinson Study Group QE3 Investigators; Beal, M.F.; Oakes, D.; Shoulson, I.; Henchcliffe, C.; Galpern, W.R.; Haas, R.; Juncos, J.L.; Nutt, J.G.; Voss, T.S.; et al. A randomized clinical trial of high-dosage coenzyme Q10 in early Parkinson disease, no evidence of benefit. JAMA Neurol. 2014, 71, 543–552. [Google Scholar]
Jie, Z. Clinical effects and safety of coenzyme Q10 in Parkinson disease. China Foreign Med. Treat. 2014, 23, 79–80. [Google Scholar]
Wang, X.Y.; Yang, Z.M.; Zhang, X.J.; Xi, S.; Liu, C.; Li, J.; Wang, Q.; Wang, L. Clinical observation of coenzyme Q10 in Parkinson disease. HeBei J. TCM 2014, 36, 151–153. [Google Scholar]
Li, Z.; Wang, P.; Yu, Z.; Cong, Y.; Sun, H.; Zhang, J.; Zhang, J.; Sun, C.; Zhang, Y.; Ju, X. The effect of creatine and coenzyme q10 combination therapy on mild cognitive impairment in Parkinson’s disease. Eur. Neurol. 2015, 73, 205–211. [Google Scholar] [CrossRef] [PubMed]
Yoritaka, A.; Kawajiri, S.; Yamamoto, Y.; Nakahara, T.; Ando, M.; Hashimoto, K.; Nagase, M.; Saito, Y.; Hattori, N. Randomized, double-blind, placebo-controlled pilot trial of reduced coenzyme Q10 for Parkinson’s disease. Parkinsonism Relat. Disord. 2015, 21, 911–916. [Google Scholar] [CrossRef]
Liu, J.; Wang, L.; Zhan, S.Y.; Xia, Y. Coenzyme Q10 for Parkinson’s disease. Cochrane Database Syst. Rev. 2011, 12, CD008150, Update in Cochrane Database Syst. Rev. 2012, 5, CD008150. [Google Scholar]
Negida, A.; Menshawy, A.; El Ashal, G.; Elfouly, Y.; Hani, Y.; Hegazy, Y.; El Ghonimy, S.; Fouda, S.; Rashad, Y. Coenzyme Q10 for Patients with Parkinson’s Disease: A Systematic Review and Meta-Analysis. CNS Neurol. Disord. Drug Targets 2016, 15, 45–53. [Google Scholar] [CrossRef]
Zhu, Z.G.; Sun, M.X.; Zhang, W.L.; Wang, W.W.; Jin, Y.M.; Xie, C.L. The efficacy and safety of coenzyme Q10 in Parkinson’s disease: A meta-analysis of randomized controlled trials. Neurol. Sci. 2017, 38, 215–224. [Google Scholar] [CrossRef]
Mitsui, J.; Koguchi, K.; Momose, T.; Takahashi, M.; Matsukawa, T.; Yasuda, T.; Tokushige, S.I.; Ishiura, H.; Goto, J.; Nakazaki, S.; et al. Three-Year Follow-Up of High-Dose Ubiquinol Supplementation in a Case of Familial Multiple System Atrophy with Compound Heterozygous COQ2 Mutations. Cerebellum. 2017, 16, 664–672. [Google Scholar] [CrossRef] [Green Version]
Stamelou, M.; Reuss, A.; Pilatus, U.; Magerkurth, J.; Niklowitz, P.; Eggert, K.M.; Krisp, A.; Menke, T.; Schade-Brittinger, C.; Oertel, W.H.; et al. Short-term effects of coenzyme Q10 in progressive supranuclear palsy: A randomized, placebo-controlled trial. Mov. Disord. 2008, 23, 942–949. [Google Scholar] [CrossRef]
Apetauerova, D.; Scala, S.A.; Hamill, R.W.; Simon, D.K.; Pathak, S.; Ruthazer, R.; Standaert, D.G.; Yacoubian, T.A. CoQ10 in progressive supranuclear palsy: A randomized, placebo-controlled, double-blind trial. Neurol. Neuroimmunol. Neuroinflamm. 2016, 3, e266. [Google Scholar] [CrossRef] [Green Version]
Beal, M.F. Coenzyme Q10 administration and its potential for treatment of neurodegenerative diseases. Biofactors 1999, 9, 261–266. [Google Scholar] [CrossRef]
Shults, C.W.; Haas, R.H.; Beal, M.F. A possible role of coenzyme Q10 in the etiology and treatment of Parkinson’s disease. Biofactors 1999, 9, 267–272. [Google Scholar] [CrossRef]
Beal, M.F.; Matthews, R.T.; Tieleman, A.; Shults, C.W. Coenzyme Q10 attenuates the 1-methyl-4-phenyl-1.;2.;3.; Tetrahydropyridine (MPTP) induced loss of striatal dopamine and dopaminergic axons in aged mice. Brain Res. 1998, 783, 109–114. [Google Scholar] [CrossRef]
Cleren, C.; Yang, L.; Lorenzo, B.; Calingasan, N.Y.; Schomer, A.; Sireci, A.; Wille, E.J.; Beal, M.F. Therapeutic effects of coenzyme Q10 (CoQ10) and reduced CoQ10 in the MPTP model of Parkinsonism. J. Neurochem. 2008, 104, 1613–1621. [Google Scholar] [CrossRef]
Kobayashi, S.; Muroyama, A.; Matsushima, H.; Yoshimura, I.; Mitsumoto, Y. Oral administration of coenzyme Q10 reduces MPTP-induced loss of dopaminergic nerve terminals in the striatum in mice. Neurol. Sci. 2012, 33, 195–199. [Google Scholar] [CrossRef]
Yan, A.; Liu, Z.; Song, L.; Wang, X.; Zhang, Y.; Wu, N.; Lin, J.; Liu, Y.; Liu, Z. Idebenone Alleviates Neuroinflammation and Modulates Microglial Polarization in LPS-Stimulated BV2 Cells and MPTP-Induced Parkinson’s Disease Mice. Front. Cell. Neurosci. 2019, 12, 529. [Google Scholar] [CrossRef]
Horvath, T.L.; Diano, S.; Leranth, C.; Garcia-Segura, L.M.; Cowley, M.A.; Shanabrough, M.; Elsworth, J.D.; Sotonyi, P.; Roth, R.H.; Dietrich, E.H.; et al. Coenzyme Q induces nigral mitochondrial uncoupling and prevents dopamine cell loss in a primate model of Parkinson’s disease. Endocrinology 2003, 144, 2757–2760. [Google Scholar] [CrossRef] [Green Version]
Yang, L.; Calingasan, N.Y.; Wille, E.J.; Cormier, K.; Smith, K.; Ferrante, R.J.; Beal, M.F. Combination therapy with coenzyme Q10 and creatine produces additive neuroprotective effects in models of Parkinson’s and Huntington’s diseases. J. Neurochem. 2009, 109, 1427–1439. [Google Scholar] [CrossRef] [Green Version]
Schulz, J.B.; Henshaw, D.R.; Matthews, R.T.; Beal, M.F. Coenzyme Q10 and nicotinamide and a free radical spin trap protect against MPTP neurotoxicity. Exp. Neurol. 1995, 132, 279–283. [Google Scholar] [CrossRef]
Ghasemloo, E.; Mostafavi, H.; Hosseini, M.; Forouzandeh, M.; Eskandari, M.; Mousavi, S.S. Neuroprotective effects of coenzyme Q10 in Parkinson’s model via a novel Q10/miR-149-5p/MMPs pathway. Metab. Brain Dis. 2021, 36, 2089–2100. [Google Scholar] [CrossRef]
Nezhadi, A.; Ghazi, F.; Rassoli, H.; Bakhtiari, M.; Ataiy, Z.; Soleimani, S.; Mehdizadeh, M. BMSC and CoQ10 improve behavioural recovery and histological outcome in rat model of Parkinson’s disease. Pathophysiology 2011, 18, 317–324. [Google Scholar] [CrossRef] [PubMed]
Kooncumchoo, P.; Sharma, S.; Porter, J.; Govitrapong, P.; Ebadi, M. Coenzyme Q(10) provides neuroprotection in iron-induced apoptosis in dopaminergic neurons. J. Mol. Neurosci. 2006, 28, 125–141. [Google Scholar] [CrossRef]
Attia, H.N.; Maklad, Y.A. Neuroprotective effects of coenzyme Q10 on paraquat-induced Parkinson’s disease in experimental animals. Behav. Pharmacol. 2018, 29, 79–86. [Google Scholar] [CrossRef] [PubMed]
Somayajulu-Niţu, M.; Sandhu, J.K.; Cohen, J.; Sikorska, M.; Sridhar, T.S.; Matei, A.; Borowy-Borowski, H.; Pandey, S. Paraquat induces oxidative stress.; Neuronal loss in substantia nigra region and parkinsonism in adult rats, neuroprotection and amelioration of symptoms by water-soluble formulation of coenzyme Q10. BMC Neurosci. 2009, 10, 88. [Google Scholar] [CrossRef] [Green Version]
Binukumar, B.K.; Gupta, N.; Bal, A.; Gill, K.D. Protection of dichlorvos induced oxidative stress and nigrostriatal neuronal death by chronic coenzyme Q10 pretreatment. Toxicol Appl. Pharmacol. 2011, 256, 73–82. [Google Scholar] [CrossRef]
Abdin, A.A.; Hamouda, H.E. Mechanism of the neuroprotective role of coenzyme Q10 with or without L-dopa in rotenone-induced parkinsonism. Neuropharmacology. 2008, 55, 1340–1346. [Google Scholar] [CrossRef]
Avcı, B.; Günaydın, C.; Güvenç, T.; Yavuz, C.K.; Kuruca, N.; Bilge, S.S. Idebenone Ameliorates Rotenone-Induced Parkinson’s Disease in Rats Through Decreasing Lipid Peroxidation. Neurochem. Res. 2021, 46, 513–522. [Google Scholar] [CrossRef]
Moon, Y.; Lee, K.H.; Park, J.H.; Geum, D.; Kim, K. Mitochondrial membrane depolarization and the selective death of dopaminergic neurons by rotenone, protective effect of coenzyme Q10. J. Neurochem. 2005, 93, 1199–1208. [Google Scholar] [CrossRef]
McCarthy, S.; Somayajulu, M.; Sikorska, M.; Borowy-Borowski, H.; Pandey, S. Paraquat induces oxidative stress and neuronal cell death; neuroprotection by water-soluble Coenzyme Q10. Toxicol. Appl. Pharmacol. 2004, 201, 21–31. [Google Scholar] [CrossRef]
Turton, N.; Heaton, R.A.; Ismail, F.; Roberts, S.; Nelder, S.; Phillips, S.; Hargreaves, I.P. The Effect of Organophosphate Exposure on Neuronal Cell Coenzyme Q₁₀ Status. Neurochem. Res. 2001, 46, 131–139. [Google Scholar] [CrossRef] [Green Version]
Onaolapo, O.J.; Odeniyi, A.O.; Jonathan, S.O.; Samuel, M.O.; Amadiegwu, D.; Olawale, A.; Tiamiyu, A.O.; Ojo, F.O.; Yahaya, H.A.; Ayeni, O.J.; et al. An Investigation of the Anti-Parkinsonism Potential of Co-enzyme Q₁₀ and Co-enzyme Q₁₀/Levodopa-carbidopa Combination in Mice. Curr. Aging Sci. 2021, 14, 62–75. [Google Scholar] [CrossRef]
Gupta, B.K.; Kumar, S.; Kaur, H.; Ali, J.; Baboota, S. Attenuation of Oxidative Damage by Coenzyme Q₁₀ Loaded Nanoemulsion Through Oral Route for the Management of Parkinson’s Disease. Rejuvenation Res. 2018, 21, 232–248. [Google Scholar] [CrossRef]
Faust, K.; Gehrke, S.; Yang, Y.; Yang, L.; Beal, M.F.; Lu, B. Neuroprotective effects of compounds with antioxidant and anti-inflammatory properties in a Drosophila model of Parkinson’s disease. BMC Neurosci. 2009, 10, 109. [Google Scholar] [CrossRef] [Green Version]
Tai, K.K.; Pham, L.; Truong, D.D. Idebenone induces apoptotic cell death in the human dopaminergic neuroblastoma SHSY-5Y cells. Neurotox. Res. 2011, 20, 321–328. [Google Scholar] [CrossRef]
Shi, H.; Noguchi, N.; Xu, Y.; Niki, E. 1-Methyl-4-phenyl-2.;3-dihydropyridinium is transformed by ubiquinone to the selective nigrostriatal toxin 1-methyl-4-phenylpyridinium. FEBS Lett. 1999, 461, 196–200. [Google Scholar] [CrossRef] [Green Version]
Lönnrot, K.; Metsä-Ketelä, T.; Molnár, G.; Ahonen, J.P.; Latvala, M.; Peltola, J.; Pietilä, T.; Alho, H. The effect of ascorbate and ubiquinone supplementation on plasma and CSF total antioxidant capacity. Free Radic. Biol. Med. 1996, 21, 211–217. [Google Scholar] [CrossRef]
Shults, C.W.; Flint Beal, M.; Song, D.; Fontaine, D. Pilot trial of high dosages of coenzyme Q10 in patients with Parkinson’s disease. Exp. Neurol. 2004, 188, 491–494. [Google Scholar] [CrossRef]
Nukui, K.; Yamagishi, T.; Miyawaki, H.; Kettawan, A.; Okamoto, T.; Belardinelli, R.; Tiano, L.; Littarru, G.P.; Sato, K. Blood CoQ10 levels and safety profile after single-dose or chronic administration of PureSorb-Q40, animal and human studies. Biofactors. 2008, 32, 209–219. [Google Scholar] [CrossRef]
Seet, R.C.; Lim, E.C.; Tan, J.J.; Quek, A.M.; Chow, A.W.; Chong, W.L.; Ng, M.P.; Ong, C.N.; Halliwell, B. Does high-dose coenzyme Q10 improve oxidative damage and clinical outcomes in Parkinson’s disease? Antioxid. Redox Signal. 2014, 21, 211–217. [Google Scholar] [CrossRef]
Prasuhn, J.; Brüggemann, N.; Hessler, N.; Berg, D.; Gasser, T.; Brockmann, K.; Olbrich, D.; Ziegler, A.; König, I.R.; Klein, C.; et al. An omics-based strategy using coenzyme Q10 in patients with Parkinson’s disease, concept evaluation in a double-blind randomized placebo-controlled parallel group trial. Neurol. Res. Pract. 2019, 1, 31. [Google Scholar] [CrossRef] [Green Version]

Figure 1. Chemical structure of coenzyme Q₁₀.

Figure 2. PRISMA Flowchart for the studies assessing tissue concentrations of coenzyme Q10 in parkinsonian syndromes, and for therapeutic trials with CoQ10 in parkinsonian syndromes.

Figure 3. Meta-analyses of studies assessing serum/plasma total CoQ₁₀ levels [7,8,9,10,11,12,13], serum/plasma CoQ₁₀ corrected to cholesterol levels (b) [7,12], and serum/plasma oxidized CoQ₁₀/total CoQ₁₀ ratio (c) in PD patients compared with controls [9,11].

Figure 4. Meta-analyses of studies assessing CoQ₁₀ concentrations in the cerebellar cortex of PD patients and controls [18,19,20].

Figure 5. Meta-analyses of studies assessing serum/plasma total CoQ₁₀ levels, serum/plasma total CoQ₁₀ concentrations in MSA patients and controls [12,13,22].

Table 1. Coenzyme Q10 Concentrations in Several Tissues from Parkinson’s Disease (PD) Patients and Healthy Controls (HC).

Tissue	Author, Year [Ref]	Parameter	PD N	PD Mean ± SD (Except % in *)	HC N	HC Mean ± SD	Difference in Means (95% C.I.), p
Serum/plasma	Jiménez-Jiménez et al., 2000 [9]	Total CoQ₁₀ (nmol/L)	33	1157 ± 344	31	1219 ± 424	62.00 (−130.39 to 254.39); 0.522
	Buhmann et al., 2004 [10]	Total CoQ₁₀ (nmol/L)	40	990 ± 620	24	530 ± 290	−460.00 (−729.67 to −190.32); 0.001
	Sohmiya et al., 2004 [11]	Total CoQ₁₀ (nmol/L)	36	613.3 ± 160	29	748.7 ± 224	135.40 (40.11 to 230.69); 0.006
	Bolner et al., 2006 [12]	Total CoQ₁₀ (nmol/L)	44	814.28 ± 750.57	21	1004.24 ± 772.58	189.96 (−211.60 to 591.52); 0.348
	Gorgone et al., 2012 [13]	Total CoQ₁₀ (nmol/L)	82	713.49 ± 187.64	60	871.01 ± 162.16	157.52 (97.95 to 217.09); <0.001
	Kasai et al. 2016 [14]	Total CoQ₁₀ (nmol/L)	20	740.8 ± 377.2	18	985.3 ± 939.4	244.50 (−217.59 to 706.59); 0.290
	Du et al., 2018 [15]	Total CoQ₁₀ (nmol/L)	30	1640.13 ± 419.80	30	1838.58 ± 481.41	198.45 (−34.98 to 431.88); 0.094
	TOTAL SERIES	Total CoQ₁₀ (nmol/L)	285	906.01 ± 531.19	213	1025.65 ± 592.90	Random-effects model p = 0.234
	Jiménez-Jiménez et al., 2000 [9]	Total CoQ₁₀/cholesterol	33	5.03 ± 1.50	31	5.30 ± 1.84	02.7 (−0.57 to 1.11); 0.521
	Kasai et al. 2016 [14]	Total CoQ₁₀/cholesterol	20	4.07 ± 1.84	18	5.92 ± 5.88	1.85 (−0.47 to 4.17); 0.115
	TOTAL SERIES	Total CoQ₁₀/cholesterol	53	4.67 ± 1.69	49	5.53 ± 3.80	Random-effects model p = 0.197
	Sohmiya et al., 2004 [11]	% Oxidized/total CoQ₁₀	36	4.7 ± 1.8	29	3.4 ± 0.9	−1.30 (−2.03 to −0.57); <0.001
	Gorgone et al., 2012 [13]	% Oxidized/total CoQ₁₀	82	5.5 ± 0.9	60	3.8 ± 0.9	−1.70 (−2.00 to −1.40); <0.001
	TOTAL SERIES	% Oxidized/total CoQ₁₀	118	5.26 ± 1.29	89	3.67 ± 0.9	Random-effects model p = 0.006
	Sohmiya et al., 2004 [11]	Oxidized CoQ₁₀ (nmol/L)	36	28.3 ± 10.5	29	24.7 ± 8.3	−3.60 (−8.38 to 1.18); 0.137
	Kasai et al. 2016 [14]	Oxidized CoQ₁₀ (nmol/L)	20	644.2 ± 382.4	18	900.2 ± 890.6	256.00 (−186.86 to 698.86); 0.249
	Sohmiya et al., 2004 [11]	Reduced CoQ₁₀ (nmol/L)	36	585 ± 155	29	724 ± 219	139.00 (46.17 to 231.83); 0.004
	Kasai et al. 2016 [14]	Reduced CoQ₁₀ (nmol/L)	20	96.6 ± 118.2	18	85.2 ± 66.6	−11.40 (−75.52 to 52.72); 0.721
Platelets	Götz et al., 2000 [16]	Total CoQ₁₀ (ng/10⁹ platelets)	20	80.6 ± 5.9	19	93.7 ± 5.1	13.10 (9.51 to 16.69); <0.001
	Götz et al., 2000 [16]	Reduced CoQ₁₀ (ng/10⁹ platelets)	20	10.3 ± 2.4	19	20.3 ± 3.2	10.00 (8.17 to 11.83); <0.001
	Götz et al., 2000 [16]	Oxidized CoQ₁₀ (ng/10⁹ platelets)	20	70.3 ± 4.8	19	73.5 ± 4.7	3.20 (0.07 to 6.33); 0.045
	Götz et al., 2000 [16]	Reduced/oxidizedCoQ₁₀	20	0.15 ± 0.04	19	0.32 ± 0.07	0.17 (0.13 to 0.21); <0.001
	Götz et al., 2000 [16]	Reduced/total CoQ₁₀	20	0.11 ± 0.02	19	0.21 ± 0.03	0.10 (0.08 to 0.12); <0.001
Lymphocytes	Mischley et al., 2012 [17] *	% of patients with CoQ₁₀ deficiency *	22	32–36%	88	8–9%	p = 0.0012–0.006 (according to authors data)
CSF	Isobe et al., 2007 [18]	Oxidized CoQ₁₀ (nmol/L)	20	5.2 ± 1.5	17	2.9 ± 1.3	−2.30 (−3.25 to −1.35); <0.001
	Isobe et al., 2007 [18]	Reduced CoQ₁₀ (nmol/L)	20	0.7 ± 0.6	17	0.8 ± 0.7	0.10 (−0.33 to 0.53); 0.643
	Isobe et al., 2007 [18]	Oxidized/total CoQ₁₀	20	0.803 ± 0.179	17	0.682 ± 0.204	−0.12 (−0.25 to 0.01); 0.063
	Compta et al., 2018 [19]	Total CoQ₁₀ (nmol/L)	15	54.39 ± 7.16	15	36.02 ± 7.20	−18.37 (−23.74 to −13.00); < 0.001
Brain
Striatum	Hargreaves et al. 2008 [20]	Total CoQ₁₀ (pmol/mg protein)	20	188.6 ± 51.4	20	214.3 ± 64.3	25.70 (−11.56 to 62.96); 0.171
Substantia nigra	Hargreaves et al. 2008 [20]	Total CoQ₁₀ (pmol/mg protein)	8	102.9 ± 42.9	8	120.0 ± 4.3	17.10 (−15.59 to 49.79); 0.281
Cerebellum cortex	Hargreaves et al. 2008 [20]	Total CoQ₁₀ (pmol/mg protein)	25	107.1 ± 34.3	25	124.3 ± 47.1	17.20 (−6.23 to 40.63); 0.147
	Schottlaender et al., 2016 [21]	Total CoQ₁₀ (pmol/mg protein)	7	262.47 ± 28.84	37	241.87 ± 57.70	−2.06 (−65.95 to 24.75); 0.365
	Barca et al., 2016 [22]	Total CoQ₁₀ (pmol/mg protein)	9	132.2 ± 8.47	12	113.1 ± 7.16	−19.10 (−26.24 to −11.96); <0.001
	TOTAL SERIES	Total CoQ₁₀ (pmol/mg protein)	41	139.14 ± 64.49	74	181.27 ± 78.20	Random-effects model p = 0.03358
Cerebral cortex	Hargreaves et al. 2008 [20]	Total CoQ₁₀ (pmol/mg protein)	13	128.6 ± 61.4	13	218.6 ± 55.7	90.00 (42.55 to 137.45); 0.0007
	Schottlaender et al., 2016 [21]	Total CoQ₁₀ (pmol/mg)	7	276.02 ± 71.37	37	259.39 ± 107.09	−16.63 (−102.09 to 68.84); 0.697
	TOTAL SERIES	Total CoQ₁₀ (pmol/mg)	20	180.20 ± 99.89	50	248.78 ± 97.53	Random-effects model p = 0.143
Skin fibroblasts	Del Hoyo et al., 2010 [23]	Total CoQ₁₀/CS	20	1.16 ± 0.33	19	0.97 ± 0.25	−0.19 (−0.38 to 0.00); 0.051
	Del Hoyo et al., 2010 [23]	Reduced CoQ₁₀/CS	20	0.41 ± 0.16	19	0.34 ± 0.11	−0.07 (−0.16 to 0.02); 0.122
	Del Hoyo et al., 2010 [23]	Oxidized CoQ₁₀/CS	20	0.75 ± 0.26	19	0.63 ± 0.23	−0.12 (−0.28 to 0.04); 0.136
	Del Hoyo et al., 2010 [23]	Total CoQ₁₀/mg protein	20	86.27 ± 29.07	19	71.86 ± 26.38	−14.41 (−32.45 to 3.63); 0.114
	Del Hoyo et al., 2010 [23]	Reduced CoQ₁₀/mg protein	20	24.50 ± 7.38	19	24.50 ± 7.38	0.00 (−4.79 to 4.79); 1.000
	Del Hoyo et al., 2010 [23]	Oxidized CoQ₁₀/mg protein	20	56.49 ± 25.20	19	47.31 ± 23.50	−9.18 (−25.01 to 6.65); 0.248
	Del Hoyo et al., 2010 [23]	Oxidized CoQ₁₀/Reduced CoQ₁₀	20	0.60 ± 0.27	19	0.62 ± 0.27	0.02 (−0.16 to 0.20); 0.818

* Expressed in % of patients with CoQ₁₀ defficiency.

Table 2. Coenzyme Q10 Concentrations in Several Tissues from Patients with Multisystem Atrophy (MSA) and Healthy Controls (HC).

Tissue	Author, Year [Ref]	Parameter	MSA N	MSA Mean ± SD	HC N	HC Mean ± SD	Difference in Means (95% C.I.), p
Serum/plasma	Kasai et al. 2016 [14]	Total CoQ₁₀ (nmol/L)	18	593.2 ± 222.6	18	985.3 ± 939.4	392.10 (−70.34 to 854.54); 0.094
	Mitsui et al., 2016 [24]	Total CoQ₁₀ (nmol/L)	44	590.71 ± 254.82	39	833.95 ± 664.69	243.24 (28.09 to 458.39); 0.027
	Du et al., 2018 [15]	Total CoQ₁₀ (nmol/L)	30	1640.13 ± 419.80	30	1858.38 ± 481.41	218.25 (−15.18 to 451.68); 0.066
	TOTAL SERIES	Total CoQ₁₀ (nmol/L)	92	933.40 ± 583.47	87	1218.52 ± 817.98	Random-effects model p = 0.001
	Kasai et al. 2016 [14]	Total CoQ₁₀/cholesterol	18	3.04 ± 1.23	18	5.92 ± 5.88	2.88 (0.00 to 5.76); 0.050
	Kasai et al. 2016 [14]	Oxidized CoQ₁₀ (nmol/L)	18	520.7 ± 202.8	18	900.2 ± 890.6	379.50 (−58.02 to 817.02); 0.087
	Kasai et al. 2016 [14]	Reduced CoQ₁₀ (nmol/L)	18	72.4 ± 34.1	18	85.2 ± 66.6	12.80 (17.64 to 48.64); 0.473
CSF	Compta et al., 2018 [19]	Total CoQ₁₀ (nmol/L)	20	26.63 ± 3.70	15	36.02 ± 7.10	9.37 (5.61 to 13.13); <0.0001
Brain
Cerebellum cortex	Schottlaender et al., 2016 [21]	Total CoQ₁₀ (pmol/mg)	20	169.30 ± 49.71	37	241.87 ± 57.70	72.57 (41.94 to 103.20); <0.001
	Barca et al., 2016 [22]	Total CoQ₁₀ (pmol/mg)	12	68.1 ± 10.03	12	113.1 ± 7.16	45.00 (37.62 to 52.38); <0.001
	TOTAL SERIES	Total CoQ₁₀ (pmol/mg)	32	131.35 ± 63.47	49	210.33 ± 75.09	Random-effects model p = 0.0977
Cerebral cortex frontal	Schottlaender et al., 2016 [21]	Total CoQ₁₀ (pmol/mg)	20	260.44 ± 70.22	37	259.39 ± 107.09	−1.05 (−54.43 to 52.33); 0.969
Cerebral cortex occipital	Barca et al., 2016 [22]	Total CoQ₁₀ (nmol/mg protein)	10	277.1 ± 29.73	9	267.3 ± 21.88	−9.80 (−35.32 to 15.72); 0.429
Striatum	Barca et al., 2016 [22]	Total CoQ₁₀ (nmol/mg protein)	7	244.2 ± 27.16	7	230.8 ± 28.62	−13.40 (−45.89 to 10.09); 0.387
Skin fibroblasts	Monzio Compagnoni et al., 2010 [25]	Total CoQ₁₀ (pg/mg protein)	14	27.83 ± 1.44	6	45.22 ± 3.48	17.39 (15.13 to 19.65); <0.001

Table 3. Coenzyme Q10 Concentrations in Several Tissues from Patients with Lewy Body Dementia (LBD), Progressive Supranuclear Palsy, and Cortical Basal Degeneration Compared with Healthy Controls (HC).

Lewy Body Dementia (LBD)
Tissue	Author, Year [Ref]	Parameter	LBD N	LBD Mean ± SD	HC N	HC Mean ± SD	Difference in Means (95% C.I.), p
Serum/plasma	Molina et al., 2002 [26]	Total CoQ₁₀ (nmol/L)	18	960.6 ± 359.1	20	1205.2 ± 362.2	244.60 (6.90 to 482.30); 0.044
	Gironi et al. 2011 [27]	Total CoQ₁₀ (nmol/L)	7	645.17 ± 290	66	622.12 ± 227.14	−23.05 (−207.81 to 161.71); 0.804
	TOTAL SERIES	Total CoQ₁₀ (nmol/L)	25	872.28 ± 365.05	86	757.72 ± 360.79	Random-effects model: p = 0.409
	Molina et al., 2002 [7]	Total CoQ₁₀/cholesterol	18	4.67 ± 1.75	20	5.05 ± 1.52	0.38 (−0.70 to 1.46); 0.478
Brain
Cerebellum cortex	Schottlaender et al., 2016 [21]	Total CoQ₁₀ (pmol/mg)	20	169.30 ± 49.71	37	241.87 ± 57.70	72.57 (41.94 to 103.20); <0.001
Cerebral cortex frontal	Schottlaender et al., 2016 [21]	Total CoQ₁₀ (pmol/mg)	20	260.44 ± 70.22	37	259.39 ± 107.09	−1.05 (−54.43 to 52.33); 0.969
Progressive Supranuclear Palsy (PSP)
Tissue	Author, Year [Ref]	Parameter	PSP N	PSP Mean ± SD	HC N	HC Mean ± SD	Difference in Means (95% C.I.), p
CSF	Compta et al., 2018 [19]	Total CoQ₁₀ (nmol/L)	10	47.67 ± 4.05	15	36.02 ± 7.10	−11.65 (−16.79 to −6.51); 0.0001
Cortical Basal Degeneration (CBD)
TISSUE	Author, Year [Ref]	Parameter	CBD N	CBD Mean ± SD	HC N	HC Mean ± SD	Difference in Means (95% C.I.), p
Cerebellum cortex	Schottlaender et al., 2016 [21]	Total CoQ₁₀ (pmol/mg)	15	271.18 ± 76.21	37	241.87 ± 57.70	−29.31 (−68.31 to 9.69); 0.137

Table 4. Studies describing the effects of levodopa and dopamine agonists in patients with RBD.

Authors, Year [Ref]	Study Setting	Type of Study	Main Findings	Level of Evidence (Quality Score)
Strijks et al., 1997 [29]	10 patients diagnosed with PD. Dosage of 200 mg/day. Assessment of motor performance with UPDRS and motor test.	3 months open-label study	Lack of improvement in PD motor symptoms.	II (NA)
Shults et al., 2002 [30]	Eighty subjects with early PD not requiring treatment for their disability. Dosages of 300, 600, or 1200 mg/day Evaluation with the UPDRS at the screening, baseline, and 1-, 4-, 8-, 12-, and 16-month visits. Follow-up of 16 months or until disability requiring treatment with levodopa.	Multicenter, randomized, parallel-group, placebo-controlled, double-blind, dosage-ranging trial.	Significantly lower increase in UPDRS scores during follow-up in patients assigned to CoQ₁₀ therapy, especially with the highest doses.	I (>50%)
Müller et al., 2003 [31]	Twenty-eight treated and stable PD patients. Dosage of 360 mg/day for 4 weeks. Scoring of PD symptoms, and visual function using the Farnsworth–Munsell 100 Hue test (FMT).	Monocenter, parallel-group, placebo-controlled, double-blind trial	Mild symptomatic benefit on PD symptoms in patients assigned to CoQ₁₀ therapy. Better improvement in FMT performance in patients assigned to CoQ₁₀ therapy.	I (>50%)
NINDS NET-PD Investigators 2007 [32]	Seventy-one untreated early PD patients assigned to CoQ10 therapy (2400 mg/day), 71 to GPI-1485, and 71 to placebo. Measurement of change in total UPDRS scores and subscores, Hoehn & Yahr staging, and Schwabb & England scale scores, either at the time requiring symptomatic therapy or at 12 months.	Randomized, double-blind, calibrated futility clinical trial	The primary outcome measure (change in total UPDRS scores over 1 year) did not differ significantly between the 3 treatment groups. Changes in Hoehn & Yahr staging, and Schwabb & England scale scores did not differ significantly between the 3 treatment groups. CoQ₁₀ was well-tolerated. The percentages of withdrawal because of adverse effects were 8%, 11%, and 10%, respectively, for CoQ₁₀, GPI-1485, and placebo.	I (>50%)
Storch et al., 2007 [33]	One hundred thirty-one patients with PD without motor fluctuations and a stable antiparkinsonian treatment. Treatment with placebo or nanoparticular CoQ₁₀ (100 mg 3 times a day, equivalent to 1200 mg/day of standard formulation) for 3 months. The stratification criterion was levodopa treatment. Evaluation with the UPDRS (sum score of parts II and III) at baseline, 1, 2, and 3 months at each visit monthly.	Multicenter, randomized, double-blind, placebo-controlled, stratified, parallel-group, single-dose trial.	The mean changes of the sum UPDRS parts II/III score did not differ significantly between the placebo and CoQ₁₀ groups (−3.69 and −3.33) No secondary outcome measure showed a significant change between the placebo group and the CoQ₁₀ group. The frequency and quality of adverse events are similar in both treatment groups.	I (>50%)
Parkinson Study Group QE3 Investigators [34]	Six hundred patients diagnosed with PD (from 67 hospitals in the USA) in the previous 5 years, free of dopaminergic therapy in the previous 3 months, with Hoehn & Yahr stage of 2.5 or less. Two hundred were assigned to CoQ₁₀ 1200 mg/day, 200 to CoQ₁₀ 2400 mg/day and 200 to placebo. All patients were taking vitamin E 1200 IU/day. Evaluation at 16 months from baseline or until a disability requiring dopaminergic treatment. The study was powered to detect a 3-point difference between active treatment and placebo.	Phase III randomized, placebo-controlled, double-blind clinical trial	At study termination, both active treatment groups showed slight adverse trends relative to placebo. Adjusted mean changes (worsening) in total UPDRS scores from baseline to final visit did not differ between the 3 study groups. Treatments were well-tolerated with no safety concerns.	I (>50%)
Jie et al., 2014 [35]	Eighty-eight patients diagnosed with PD and treated with levodopa. Forty-four were assigned to CoQ₁₀ 375–750 mg/day, and 44 to placebo Evaluation with the Webster Scale at baseline and 3 months	Monocenter, randomized, placebo-controlled, double-blind clinical trial	Significant improvement in UPDRS Webster Scale scores in the group of patients treated with CoQ_10. Lack of significant adverse effects.	I (>50%)
Wang et al., 2014 [36]	Thirty-nine patients diagnosed with PD under conventional therapy. Twenty-one were assigned to CoQ₁₀ 450 or 1200 mg/day, and 18 to placebo as add-on therapy Evaluation with the UPDRS III and Webster Scale at baseline and 36 weeks	Monocenter, randomized, placebo-controlled, double-blind clinical trial	Significant improvement in UPDRS III and Webster Scale scores in the group of patients treated with CoQ₁₀ 1200 mg/day (but not of the patients treated with CoQ₁₀ 450 mg/day) compared with the placebo group.	I (>50%)
Li et al., 2015 [37]	Seventy-five patients diagnosed with PD and MCI. Random assignation to treatment with CoQ₁₀ 100 mg b.i.d. and creatine 5 mg b.i.d. or to placebo. Evaluation with the UPDRS part III, and MoCa at 12 and 18 months.	Phase III randomized, placebo-controlled, double-blind clinical trial	Non-significant differences in UPDRS III scores between the 2 study groups at 12 and 18 months. Significantly lower worsening in the MoCA scores in patients assigned to CoQ₁₀ plus creatine.	I (>50%)
Yoritaka et al., 2015 [38]	Twenty-six patients with PD experiencing wearing off (group A) and 22 early PD patients without levodopa (with or without a dopamine agonist, group B). Treatment with 300 mg/day of ubiquinol-10 or placebo for 48 weeks (Group A, 14 ubiquinol-10, 12 placeboes) or 96 weeks (Group B, 14 ubiquinol-10, 8 placeboes).	Randomized, double-blind, placebo-controlled, parallel-group pilot trial	Significant improvement in UPDRS scores in patients treated with ubiquinol-10 compared with placebo in group A. Lack of significant changes in UPDRS scores in patients treated with ubiquinol-10 compared with placebo in group B.	I (>50%)

MoCA: Montreal Cognitive Assessment, PD: Parkinson’s disease, UPDRS: Unified Parkinson’s disease rating scale.

Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

Coenzyme Q10 and Parkinsonian Syndromes: A Systematic Review

Abstract

1. Introduction

2. Methods

2.1. Search Strategy and Criteria for Eligibility of Studies

2.2. Selection of Studies and Methodology for the Meta-Analyses