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Diagnostics 2018, 8(3), 44; https://doi.org/10.3390/diagnostics8030044

Utility of Two-Dimensional Difference Gel Electrophoresis in Diagnosis of Multiple Sclerosis

1
Department of Neurology, Medical University of Innsbruck, Innrain 66, 6020 Innsbruck, Austria
2
Division of Biological Chemistry, Medical University of Innsbruck, Innrain 80-83, 6020 Innsbruck, Austria
*
Author to whom correspondence should be addressed.
Received: 31 May 2018 / Revised: 14 June 2018 / Accepted: 20 June 2018 / Published: 5 July 2018
(This article belongs to the Collection Biomarkers in Medicine)
Full-Text   |   PDF [223 KB, uploaded 5 July 2018]

Abstract

Two-dimensional difference gel electrophoresis (2D-DIGE) has been used for identification of possible biomarkers in the cerebrospinal fluid (CSF) of multiple sclerosis (MS) patients. However, in different studies inconsistent results have been obtained. We wanted to analyze the diagnostic value of 2D-DIGE in early MS patients by comparing protein patterns between single and pooled samples of MS patients and controls. CSF samples of 20 MS patients and 10 control subjects were processed with 2D-DIGE. The so obtained protein patterns were analyzed with DeCyder 6.5 software, whereby we described variation of patterns presented in one gel as well as between different gels. Even when running single samples of patients of the same group in one gel, variation of protein patterns was high. The number of identified spots with different protein level varied between 4 and 30, depending on which sample batches were compared. We did not find a consistent pattern throughout all possible batch combinations. The inter-individual variation of protein expression as well as the susceptibility of 2D-DIGE for methodological variations makes use of 2D-DIGE as a diagnostic tool for MS and for detection of possible candidate biomarkers difficult, since detected proteins vary depending on which samples are compared. View Full-Text
Keywords: 2D-DIGE; diagnosis; electrophoresis; multiple sclerosis; protein 2D-DIGE; diagnosis; electrophoresis; multiple sclerosis; protein
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Auer, M.; Hegen, H.; Rudzki, D.; Golderer, G.; Deisenhammer, F. Utility of Two-Dimensional Difference Gel Electrophoresis in Diagnosis of Multiple Sclerosis. Diagnostics 2018, 8, 44.

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