Next Article in Journal
Natriuretic Peptides in Kawasaki Disease: the Myocardial Perspective
Next Article in Special Issue
A Review of Heating and Temperature Control in Microfluidic Systems: Techniques and Applications
Previous Article in Journal / Special Issue
Cell-Based Biosensors: Electrical Sensing in Microfluidic Devices
Open AccessArticle

Detection of Dissolved Lactose Employing an Optofluidic Micro-System

1
Institute for Microsensors, Actuators and Systems (IMSAS), Microsystems Center Bremen (MCB), University of Bremen, Otto-Hahn-Allee NW1, 28359 Bremen, Germany
2
Institute of Sensor and Actuator Systems, Vienna University of Technology, Gusshausstrasse 27-29,E366, 1040 Vienna, Austria
*
Author to whom correspondence should be addressed.
Diagnostics 2012, 2(4), 97-106; https://doi.org/10.3390/diagnostics2040097
Received: 2 October 2012 / Revised: 26 November 2012 / Accepted: 3 December 2012 / Published: 6 December 2012
(This article belongs to the Special Issue Microfluidic Lab-on-a-Chip Platforms for High-Performance Diagnostics)
In this work, a novel optofluidic sensor principle is employed for a non-invasive and label-free characterization of lactose containing liquid samples. Especially for medicine and food industry, a simple, fast and accurate determination of the amount of lactose in various products is highly desirable. The presented system exploits the impact of dissolved molecules on the refractive index for sample characterization. On the optofluidic chip, a microfluidic channel filled with the analyte is hit by slightly diverging laser light. The center incident angle of the beam on-chip is set close to the critical angle for total internal reflection. Both the reflected and the transmitted light signals are recorded at the solid-liquid interface. The ratio of those two signals is then used as representative value for the analyte. Using this principle, lactose containing samples were differentiated based on their concentrations at a step size of 10 mmol/L. The use of the signals ratio instead of a single signal approach improves the stability of the system significantly, allowing for higher resolutions to be achieved. Furthermore, the fabrication of the devices in PDMS ensures biocompatibility and provides low absorbance of light in the visible range. View Full-Text
Keywords: lactose detection; non-invasive; label-free; optofluidics; partial total internal reflection lactose detection; non-invasive; label-free; optofluidics; partial total internal reflection
Show Figures

Graphical abstract

MDPI and ACS Style

Weber, E.; Keplinger, F.; Vellekoop, M.J. Detection of Dissolved Lactose Employing an Optofluidic Micro-System. Diagnostics 2012, 2, 97-106.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop