Search Results (200)

Monoclonal antibody (mAb) discovery has been transformed by advances in single-cell technologies, microfluidics, high-throughput sequencing, and computational design. Modern platforms enable the interrogation of large numbers of individual B cells, directly linking antibody sequence with antigen specificity and functional activity. Microfluidic and optofluidic systems now support high-throughput compartmentalisation and functional screening of antibody-secreting cells, while sequencing-based approaches allow parallel recovery of paired heavy- and light-chain sequences. These developments have shifted antibody discovery from binding-based selection toward function-first paradigms, enabling the rapid identification of diagnostic and therapeutically relevant antibodies. Integration with computational tools, including machine learning and structure-based modelling, has further enabled the emergence of closed-loop discovery pipelines, in which experimental and in silico methods iteratively refine candidates. This review summarises key advances in single-cell microtools over the last decade and highlights how the convergence of experimental and computational technologies is reshaping antibody discovery toward scalable, data-driven, and increasingly automated platforms. Full article

Optical chromatography (OC) has emerged as a powerful, label-free technique for the precise manipulation and separation of micro- and nanoparticles based on their intrinsic biophysical properties, including size, refractive index, and morphology. By balancing optical radiation pressure with fluid drag forces, OC enables high-resolution sorting of diverse analytes—from synthetic colloids to biological cells and pathogens—without the need for fluorescent labels or chemical modifications. Recent advancements in integrated optofluidic platforms, such as plasmonic microlens arrays, fiber-based systems, and hybrid optical–electrical detection approaches, have significantly enhanced OC capabilities, addressing long-standing challenges in scalability, throughput, and sensitivity, and facilitated its transition toward compact, application-oriented analytical platforms. These innovations have expanded OC applications in critical biomedical fields, including exosome isolation, pathogen detection, and viral infection monitoring. Furthermore, the integration of OC with tunable resistive pulse sensing (TRPS) presents a promising avenue for simultaneous particle fractionation and characterization, overcoming key limitations of conventional resistive pulse techniques. In this review, we provide a comprehensive overview of the fundamental principles of OC, followed by recent progress in particle separation strategies and integrated optofluidic system design. We further highlight emerging applications in bioanalysis and discuss future directions toward high-throughput, multimodal, and clinically relevant OC platforms. Full article

In this work, we present a numerical proof-of-concept study of a device for nanoparticle sorting, targeting size ranges relevant to exosome-like dimensions (typically 40–200 nm), which remains challenging for passive sorting techniques. The system consists of three silicon waveguides embedded in a CYTOP layer and arranged in a two-step directional-coupler configuration, integrated with a microchannel that carries a water-based buffer as the carrier fluid, transporting the suspended nanoparticles. Three-dimensional Finite Element Method (3D-FEM) simulations were performed, incorporating both optical and hydrodynamic forces to track particle dynamics within the microchannel and demonstrate controlled, size-selective particle deflection. First, numerical simulations show that nanospheres with diameters ranging from 500 nm to 700 nm can be effectively separated by the transverse trapping force at a 4:1 power-splitting ratio. Then, to extend the concept toward smaller size ranges, a bifurcated microchannel is introduced, enabling fluid-assisted transport in low-optical-field regions and allowing reliable separation of particles with smaller diameters (between 200 nm and 400 nm), accompanied by an 8:1 power-splitting ratio. These results demonstrate, within a numerical framework, the feasibility of an integrated photonic–microfluidic approach for size-selective nanoparticle sorting. The proposed strategy may support future pre-processing steps in liquid biopsy workflows, particularly for enriching nanoscale components such as exosome-sized vesicles, rather than constituting a direct diagnostic tool. Full article

Precise manipulation and directed transport of micro- and nano-particles are cornerstones of emerging lab-on-a-chip technologies. Traditional optofluidic systems that combine optical tweezers with microfluidic channels enable long-range transport. However, they rely on fixed physical boundaries that lack reconfigurability. To bridge this gap, we propose a reconfigurable virtual optical waveguide (VOW) based on a discretized beam-shaping strategy. By superposing two orthogonally polarized shaped beams, we construct interference-free optical channels without physical boundaries. This platform enables programmable transport along complex trajectories, including space-filling Hilbert curves that maximize interaction path length, and shields the transport channel from perturbations induced by surrounding particles. Crucially, the VOW offers multi-dimensional sorting capabilities: (i) it performs precise size-dependent sieving via tunable channel widths, and (ii) it functions as an intrinsic material filter by stably guiding scattering-dominated particles (e.g., gold) while rejecting gradient-dominated dielectric ones. This work establishes a versatile, contactless strategy for adaptive optical logistics and on-chip material purification. Full article

Precise manipulation of two-phase flow in micro-confined electrowetting pixels is limited by contact angle hysteresis (CAH). To elucidate this non-equilibrium process, we establish a high-fidelity electrohydrodynamic (EHD) phase-field simulation framework. The model rigorously couples Navier–Stokes equations with molecular kinetic theory (MKT) to characterize energy dissipation at the three-phase contact line (TCL) and further integrates charge transport kinetics. Numerical results reveal CAH is driven by physical pinning and interfacial charge trapping, with the latter dominating interfacial retreat and causing significant residual displacement. Furthermore, analysis shows alternating current (AC) waveforms mitigate charge accumulation and promote depinning via micro-oscillations, minimizing the hysteresis loop compared to direct current (DC) waveforms. Additionally, an overdrive strategy utilizing a suprathreshold Maxwell stress pulse rapidly overcomes static friction. This strategy significantly improves transient dynamics, substantially reducing the time to reach 90% of the steady-state target from 19.6 ms (under standard DC waveform driving) to 7.4 ms. This work provides a comprehensive theoretical basis and design criteria for optimizing active driving strategies in optofluidic and digital microfluidic systems. Full article

Photonic crystal waveguides (PhCWs) have emerged as a leading platform for integrated optical sensing due to their ability to engineer dispersion, enhance light–matter interaction, and exploit slow-light effects. This review provides a comprehensive analysis of the fundamental physics, performance metrics, device architectures, and application domains that define the current state of PhCW-based sensing. Key mechanisms governing sensitivity, figure of merit, detection limit, and dynamic range are examined, with emphasis on the intrinsic trade-offs introduced by slow-light operation, including disorder-induced scattering, linewidth broadening, and thermal susceptibility. Advances in dispersion engineering, such as hole shifting, gentle confinement, and width modulation, are highlighted alongside novel architectures including slot PhCWs, hybrid material platforms, and plasmonic–photonic configurations. Their respective capabilities in enhancing analyte overlap, improving spectral stability, and expanding functional integration are critically assessed. Emerging applications in biochemical detection, environmental monitoring, and nanoscale particle sensing further illustrate the versatility of PhCWs within modern optofluidic and lab-on-chip systems. The review concludes by outlining key challenges and future directions, including disorder-resilient slow-light design, inverse-engineered structures, and platform-level integration, which collectively chart a path toward next-generation high-performance photonic crystal sensing technologies. Full article

Serial flow cytometry was recently introduced as a method that can estimate measurement uncertainty (i.e., imprecision, the coefficient of variation of repeated measurements of individual particles) independent from population characteristics. Replication of light sources and detectors at multiple sites along a flow cytometer’s microchannel requires more equipment and can complicate detector synchronization. Here, we introduce amplitude modulation to encode each region of a serial cytometer with a unique carrier frequency, which enables demultiplexing of the combined signal incident on a single photodetector by fast Fourier transform (FFT) peak magnitude. To facilitate validation of detection, matching, and uncertainty quantification of fluorescence signals, we designed a microfluidic amplitude modulation (AM) serial flow cytometer that has ground truth detectors on individual regions (serial cytometry) in parallel with the combined channel detection for AM demultiplexing. With this report, we present metrics for event detection and dynamic range, prevalence and processing of overlapping detections, region-decoding accuracy, process yield, and uncertainty quantification on a brightness ladder of calibration microspheres. Despite being operated with reduced light intensities, the AM cytometer was capable of high-fidelity performance in comparison to conventional serial cytometry. For events above the detection limit, over 97% were analyzed. Both conventional and AM serial cytometers achieved median imprecisions in the range of 0.53% to 2.1% after outlier removal, which was well below the inherent intensity distribution of any of the microsphere subpopulations. Overall, AM cytometry supports uncertainty quantification and temporal analyses of serial cytometry data with a reduced number of photodetectors, which offers simplification of chip design with multiple measurement regions and wide-field detectors. Full article

Cold atmospheric plasma (CAP) has emerged as a promising anticancer approach because of its ability to selectively eliminate malignant cells. Among the proposed mechanisms of this selectivity, the Bauer theory emphasizes the synergistic action of plasma-derived hydrogen peroxide (H₂O₂) and nitrite (NO₂⁻), leading to the transient generation of primary singlet oxygen (¹O₂). This early event inactivates membrane-bound catalase, allowing tumor cell-derived H₂O₂ and peroxynitrite to initiate a self-amplifying cycle that produces secondary ¹O₂, as a hallmark of CAP selectivity. To test this hypothesis, in this work, we monitored extracellular dissolved oxygen (DO) dynamics in HT-29 colorectal cancer cells treated with an argon plasma jet using time-resolved phosphorescence lifetime spectroscopy. Temporal variations in DO likely reflect the cumulative effect of rapid ¹O₂ production and its reactions with cells. A delayed surge in extracellular ¹O₂ was observed specifically in dying cancer cells within the 10–20 min window predicted by the model. Intracellular ROS imaging confirmed a strong correlation between intracellular ROS, extracellular ¹O₂ dynamics, and viability loss. Together, these results provide mechanistic validation of Bauer’s redox model and suggest that early oxygen dynamics after CAP exposure can serve as predictive markers for treatment efficacy in plasma or photodynamic therapies. Full article

The sustained interest in efficient, low-cost, and straightforward-to-manufacture lasers has prompted intense research into organic semiconductor laser emitter materials in recent decades. The main focus of this research is determining the optical gains and losses of amplified spontaneous emission (ASE) in order to describe materials by their amplification signature. A method that has been used for decades as the standard technique for determining gain characteristics is the variable-stripe-length (VSL) method. The success of the VSL method has led to the development of further measurement techniques. These techniques provide a detailed insight into the nature of optical amplification. One such method is the scattered emission profile (SEP) method. In this study, we present an extension of the SEP method, the Diffracted Emission Profile (DEP) method. The DEP method is based on the detection of ASE by partial decoupling of waveguide modes diffracted by a one-dimensional grating integrated into a planar waveguide. Diffraction causes a proportion of the intensity to exit the waveguide, transferring the growth and decay process of the waveguide mode to the transverse mode profile of the diffracted mode. In the present article, an approach to determine the amplification signature of an organic copolymer is presented, utilizing partial decoupled radiation. Full article

Plastic pollution raises concerns for health and the environment. Plastics are not biodegradable but gradually erode to microplastic and nanoplastic particles spreading almost everywhere. Nanoplastics exhibit colloidal behavior. Thereby, their analysis can be accomplished by refractometry, preferably by an on-chip tool. We present a study of such colloids using a microfabricated Fabry–Pérot cavity with curved mirrors, which holds a capillary micro-tube used both for fluid handling and light collimation, resulting in an optically stable microresonator. Despite the numerous scatterers within the sample, the sub-millimeter scale cavity provides the advantages of reduced interaction length while maintaining light confinement. This significantly reduces optical loss and hence keeps resonance modes with quality factors (resonant frequency/bandwidth) above 1100. Therefore, small quantities of colloids can be measured by the interference spectral response through the shift in resonant wavelengths. The particles’ Brownian motion potentially causing perturbations in the spectra can be overcome either by post-measurement cross-correlation analysis or by avoiding it entirely by taking the measurements at once by a wideband source and a spectrum analyzer. The effective refractive index of solutions with solid contents down to 0.34% could be determined with good agreement with theoretical predictions. Even lower detection capabilities might be attained by slightly altering the technique to cause particle aggregation achieved solely by light. Full article

Achieving controllable wavelength tuning in optofluidic whispering gallery mode microcavity lasers is crucial for high-throughput, multi-sample, multiplexed biochemical sensing and multifunctional integrated photonic devices. This paper develops a bidirectionally wavelength-tunable optofluidic fiber whispering gallery mode microcavity laser driven by Rhodamine 6G co-doped with different acceptor dyes. Experimentally, a thin-walled silica ring inside a hollow-core anti-resonant fiber served as the optical microcavity, with a fixed 2.5 mM Rhodamine 6G co-doped with other dyes as the gain medium. The results revealed that when co-doped with Rhodamine B or Cy3, the single-longitudinal-mode laser emission wavelength exhibited a red shift with increasing co-dopant concentration. Conversely, when co-doped with Cy5, the laser output wavelength showed a distinct blue shift. This unique bidirectional tuning characteristic originates from the different fluorescence resonance energy transfer efficiencies between the co-dopants and Rhodamine 6G, and their competitive modulation of the system’s effective gain spectrum. The study offers a novel and flexible strategy for achieving wide-range, controllable wavelength tuning on a single laser platform, with significant potential for applications in biochemical sensing and multifunctional integrated photonic devices. Full article

We present a parallel DNA molecular analysis platform based on an array of plano-concave Fabry–Pérot (PC-FP) microcavity lasers that enables the simultaneous, sequence-specific detection of multiple DNA targets. Each PC-FP cavity is functionalized with a distinct probe DNA and integrated within a microfluidic channel, allowing localized hybridization and lasing emission upon optical pumping. When Cy3-labeled complementary targets were introduced, distinct lasing peaks emerged from corresponding cavities at ~607 nm, whereas single-base-mismatched sequences produced no measurable signal. The lasing threshold was approximately 0.6 µJ/mm², confirming highly efficient optical feedback and cavity-enhanced signal amplification. The parallel operation of three PC-FP cavities demonstrated independent, multiplexed detection without optical crosstalk. The plano-concave geometry provides mode stability, compact alignment tolerance, and a tenfold reduction in threshold compared to flat FP mirrors. These results highlight the potential of PC-FP microcavity laser arrays as a scalable alternative to fluorescence-based assays, offering rapid, high-throughput DNA hybridization and melting analysis within a miniaturized solid-state architecture. Full article

This work is devoted to the study of the effect of the oscillating Poiseuille flow on the diffraction of light passing through a nematic layer bounded by a submicron relief at one of the inner surfaces of the plane capillary. In experimental nematic liquid crystal (NLC) cells with a hybrid planar–homeotropic orientation, a photo-profiled PAZO polymer layer with a sinusoidal relief with a depth of 180 and 360 nm and a period of 2 μm was used as a diffraction grating. The experimentally obtained dependencies of the flow-induced changes in the intensity of polarized light at the main and the first diffraction maxima on the amplitude of the low-frequency oscillating pressure gradient applied to the NLC layer are presented. Processing of the obtained results indicates the possibility of modulating the intensity of diffracted polarized light transmitted through the NLC layer by up to 10% when applying an oscillating pressure difference of up to 700 Pa to the layer of corresponding experimental cells in the absence of an analyzer in the optical scheme. Possible mechanisms responsible for the modulation of optical radiation in the main and first diffraction maxima are discussed. The discussed principles of controlling diffracted electromagnetic radiation can be used to create optofluidic modulators operating in both the visible and THz ranges. Full article