Formation of Abasic Oligomers in Nonenzymatic Polymerization of Canonical Nucleotides
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Methods
2.2.1. Dehydration-Rehydration Cycles
2.2.2. Analysis of Deglycosylation
2.2.3. HPLC Analysis
2.2.4. Mass Analysis
3. Results and Discussion
3.1. Polymerization of Canonical Nucleotides
3.2. DH-RH Reactions for Nucleotide Mixtures Capable of Hydrogen Bonding
3.3. Deglycosylation Reactions during DH-RH Cycles
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Chemical Species | Expected Mass | Observed Mass | ppm Error |
---|---|---|---|
AMP Reaction | |||
Adenine | 136.0617 | 136.0635 | 13.2290 |
AMP monomer | 348.0703 | 348.0691 | 3.4475 |
Abasic Dimer | 560.0778 | 560.0764 | 2.4996 |
Abasic Trimer | 772.0874 | 772.0899 | 3.2379 |
GMP Reaction | |||
Guanine | 152.0566 | 152.057 | 2.6305 |
GMP Monomer | 364.0652 | 364.0627 | 6.8669 |
Abasic Dimer | 576.0737 | 576.0715 | 3.8189 |
Abasic Trimer | 788.0824 | 788.0803 | 2.6646 |
UMP Reaction | |||
Uracil | 113.0345 | 113.0346 | 0.8846 |
UMP Monomer | 325.0431 | 325.0435 | 1.2306 |
Intact Dimer | 631.0684 | 631.0682 | 0.3169 |
Abasic Dimer | 537.0517 | 537.0533 | 2.9792 |
CMP Reaction | |||
Cytosine | 112.0505 | 112.0494 | 9.8170 |
CMP Monomer | 324.0591 | 324.0596 | 1.5429 |
Intact Dimer | 629.1004 | 629.1017 | 2.0664 |
Abasic Dimer | 536.0677 | 536.0677 | 0.0000 |
Chemical Species | Expected Mass | Observed Mass | ppm Error |
---|---|---|---|
AMP + UMP Reaction | |||
Adenine | 136.0617 | 136.0635 | 13.2290 |
AMP Monomer | 348.0703 | 348.0691 | 3.4475 |
Uracil | 113.0345 | 113.0346 | 0.8846 |
UMP Monomer | 325.0431 | 325.0435 | 1.2306 |
Abasic A Dimer | 560.0778 | 560.0812 | 6.0705 |
Abasic U Dimer | 537.0517 | 537.0533 | 2.9792 |
Abasic A Trimer | 772.0874 | 772.0899 | 3.2379 |
Abasic U Trimer | 749.0603 | 749.0637 | 4.5390 |
GMP + CMP Reaction | |||
Guanine | 152.0566 | 152.057 | 2.6305 |
GMP Monomer | 364.0652 | 364.0627 | 6.8669 |
Cytosine | 112.0505 | 112.0494 | 9.817 |
CMP Monomer | 324.0591 | 324.0596 | 1.5429 |
Intact CC Dimer | 629.1004 | 629.1017 | 2.0664 |
Intact CG Dimer | 669.1065 | 669.1042 | 3.4374 |
Abasic G Dimer | 576.0737 | 576.0715 | 3.8189 |
Abasic C Dimer | 536.0677 | 536.0677 | 0.0000 |
Abasic G Trimer | 788.0824 | 788.0803 | 2.6646 |
Abasic C Trimer | 748.0763 | 748.0787 | 3.2082 |
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Mungi, C.V.; Bapat, N.V.; Hongo, Y.; Rajamani, S. Formation of Abasic Oligomers in Nonenzymatic Polymerization of Canonical Nucleotides. Life 2019, 9, 57. https://doi.org/10.3390/life9030057
Mungi CV, Bapat NV, Hongo Y, Rajamani S. Formation of Abasic Oligomers in Nonenzymatic Polymerization of Canonical Nucleotides. Life. 2019; 9(3):57. https://doi.org/10.3390/life9030057
Chicago/Turabian StyleMungi, Chaitanya V., Niraja V. Bapat, Yayoi Hongo, and Sudha Rajamani. 2019. "Formation of Abasic Oligomers in Nonenzymatic Polymerization of Canonical Nucleotides" Life 9, no. 3: 57. https://doi.org/10.3390/life9030057