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Open AccessArticle

Monitoring of Cyanobacteria in Water Using Spectrophotometry and First Derivative of Absorbance

1
Institute of Environmental Science and Interdisciplinary Science, Carleton University, Ottawa, ON K1S 5B6, Canada
2
Department of Civil and Environmental Engineering, Carleton University, Ottawa, ON K1S 5B6, Canada
*
Author to whom correspondence should be addressed.
Water 2020, 12(1), 124; https://doi.org/10.3390/w12010124
Received: 19 November 2019 / Revised: 20 December 2019 / Accepted: 20 December 2019 / Published: 30 December 2019
(This article belongs to the Special Issue Monitoring, Modelling and Management of Water Quality)
Management of cyanobacteria blooms and their negative impact on human and ecosystem health requires effective tools for monitoring their concentration in water bodies. This research investigated the potential of derivative spectrophotometry in detection and monitoring of cyanobacteria using toxigenic and non-toxigenic strains of Microcystis aeruginosa. Microcystis aeruginosa was quantified in deionized water and surface water using traditional spectrophotometry and the first derivative of absorbance. The first derivative of absorbance was effective in improving the signal of traditional spectrophotometry; however, it was not adequate in differentiating between signal and noise at low concentrations. Savitzky-Golay coefficients for first derivative were used to smooth the derivative spectra and improve the correlation between concentration and noise at low concentrations. Derivative spectrophotometry improved the detection limit as much as eight times in deionized water and as much as four times in surface water. The lowest detection limit measured in surface water with traditional spectrophotometry was 392,982 cells/mL, and the Savitzky-Golay first derivative of absorbance was 90,231 cells/mL. The method provided herein provides a promising tool in real-time monitoring of cyanobacteria concentrations and spectrophotometry offers the ability to measure water quality parameters together with cyanobacteria concentrations. View Full-Text
Keywords: cyanobacteria; Microcystis aeruginosa; water; monitoring; spectrophotometry; derivative absorbance cyanobacteria; Microcystis aeruginosa; water; monitoring; spectrophotometry; derivative absorbance
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MDPI and ACS Style

Agberien, A.V.; Örmeci, B. Monitoring of Cyanobacteria in Water Using Spectrophotometry and First Derivative of Absorbance. Water 2020, 12, 124.

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