Developing a Biotechnological Tool for Monitoring Water Quality: In Vitro Clone Culture of the Aquatic Moss Fontinalis Antipyretica

Debén, Sofía; Aboal, Jesús Ramón; Giráldez, Pablo; Varela, Zulema; Fernández, Jose Ángel

doi:10.3390/w11010145

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Developing a Biotechnological Tool for Monitoring Water Quality: In Vitro Clone Culture of the Aquatic Moss Fontinalis Antipyretica

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Ecology Unit, Department of Functional Biology, Universidade de Santiago de Compostela, Fac. Bioloxía, Lope Gómez de Marzoa s/n, Santiago de Compostela, 15702 A Coruña, Spain

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Water 2019, 11(1), 145; https://doi.org/10.3390/w11010145

Received: 18 December 2018 / Revised: 10 January 2019 / Accepted: 12 January 2019 / Published: 15 January 2019

(This article belongs to the Special Issue Biomonitoring of Water Quality)

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Abstract

One of the main factors limiting active biomonitoring with aquatic mosses is the lack of sufficient material. A laboratory culture of the moss would solve this problem and thus convert the technique into a valuable biotechnological tool for monitoring water quality. With this aim, we first established small and large scale axenic in vitro culture systems for the aquatic moss Fontinalis antipyretica. We then attempted to enhance the growth rate of the cultures by modifying temperature, photoperiod and medium composition (N:P ratio, P concentration, CO₂ supply, NH₄NO₃ supply and sucrose supply). None of these modifications greatly increased the in vitro growth rate. However, the growth rates were sufficiently high (relative to the initial weight of the cultures) in both systems (45 and 6 mg·day⁻¹·g⁻¹ for flasks and bioreactors respectively) to enable the production of large amounts of material. The ability to culture the material will substantially improve the applicability of the moss bag technique. View Full-Text

Keywords: moss bag technique; bioreactors; bryophytes; Fontinalis propagule; clonal propagation

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MDPI and ACS Style

Debén, S.; Aboal, J.R.; Giráldez, P.; Varela, Z.; Fernández, J.Á. Developing a Biotechnological Tool for Monitoring Water Quality: In Vitro Clone Culture of the Aquatic Moss Fontinalis Antipyretica. Water 2019, 11, 145. https://doi.org/10.3390/w11010145

AMA Style

Debén S, Aboal JR, Giráldez P, Varela Z, Fernández JÁ. Developing a Biotechnological Tool for Monitoring Water Quality: In Vitro Clone Culture of the Aquatic Moss Fontinalis Antipyretica. Water. 2019; 11(1):145. https://doi.org/10.3390/w11010145

Chicago/Turabian Style

Debén, Sofía, Jesús Ramón Aboal, Pablo Giráldez, Zulema Varela, and Jose Ángel Fernández. 2019. "Developing a Biotechnological Tool for Monitoring Water Quality: In Vitro Clone Culture of the Aquatic Moss Fontinalis Antipyretica" Water 11, no. 1: 145. https://doi.org/10.3390/w11010145

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Developing a Biotechnological Tool for Monitoring Water Quality: In Vitro Clone Culture of the Aquatic Moss Fontinalis Antipyretica

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