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Open AccessArticle

Genome Mining of Non-Conventional Yeasts: Search and Analysis of MAL Clusters and Proteins

Department of Genetics, Institute of Molecular and Cell Biology, University of Tartu, Riia 23, 51010 Tartu, Estonia
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Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Genes 2018, 9(7), 354; https://doi.org/10.3390/genes9070354
Received: 30 May 2018 / Revised: 9 July 2018 / Accepted: 12 July 2018 / Published: 16 July 2018
(This article belongs to the Section Population and Evolutionary Genetics and Genomics)
Genomic clustering of functionally related genes is rare in yeasts and other eukaryotes with only few examples available. Here, we summarize our data on a nontelomeric MAL cluster of a non-conventional methylotrophic yeast Ogataea (Hansenula) polymorpha containing genes for α-glucosidase MAL1, α-glucoside permease MAL2 and two hypothetical transcriptional activators. Using genome mining, we detected MAL clusters of varied number, position and composition in many other maltose-assimilating non-conventional yeasts from different phylogenetic groups. The highest number of MAL clusters was detected in Lipomyces starkeyi while no MAL clusters were found in Schizosaccharomyces pombe and Blastobotrys adeninivorans. Phylograms of α-glucosidases and α-glucoside transporters of yeasts agreed with phylogenesis of the respective yeast species. Substrate specificity of unstudied α-glucosidases was predicted from protein sequence analysis. Specific activities of Scheffersomycesstipitis α-glucosidases MAL7, MAL8, and MAL9 heterologously expressed in Escherichia coli confirmed the correctness of the prediction—these proteins were verified promiscuous maltase-isomaltases. α-Glucosidases of earlier diverged yeasts L. starkeyi, B. adeninivorans and S. pombe showed sequence relatedness with α-glucosidases of filamentous fungi and bacilli. View Full-Text
Keywords: α-glucosidase; maltase; isomaltase; α-glucoside permease; gene cluster; protein evolution; methylotrophic yeast; MAL-locus α-glucosidase; maltase; isomaltase; α-glucoside permease; gene cluster; protein evolution; methylotrophic yeast; MAL-locus
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MDPI and ACS Style

Viigand, K.; Põšnograjeva, K.; Visnapuu, T.; Alamäe, T. Genome Mining of Non-Conventional Yeasts: Search and Analysis of MAL Clusters and Proteins. Genes 2018, 9, 354. https://doi.org/10.3390/genes9070354

AMA Style

Viigand K, Põšnograjeva K, Visnapuu T, Alamäe T. Genome Mining of Non-Conventional Yeasts: Search and Analysis of MAL Clusters and Proteins. Genes. 2018; 9(7):354. https://doi.org/10.3390/genes9070354

Chicago/Turabian Style

Viigand, Katrin; Põšnograjeva, Kristina; Visnapuu, Triinu; Alamäe, Tiina. 2018. "Genome Mining of Non-Conventional Yeasts: Search and Analysis of MAL Clusters and Proteins" Genes 9, no. 7: 354. https://doi.org/10.3390/genes9070354

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