Next Article in Journal
A Novel Probability Model for LncRNA–Disease Association Prediction Based on the Naïve Bayesian Classifier
Next Article in Special Issue
Possible Role of Envelope Components in the Extreme Copper Resistance of the Biomining Acidithiobacillus ferrooxidans
Previous Article in Journal
Epistatic Interactions in NS5A of Hepatitis C Virus Suggest Drug Resistance Mechanisms
Article

Using a Chemical Genetic Screen to Enhance Our Understanding of the Antibacterial Properties of Silver

1
Department of Biological Sciences, University of Calgary, 2500 University Dr. NW, Calgary, AB T2N 1N4, Canada
2
Department of Mathematics and Statistics, University of Calgary, 2500 University Dr. NW, Calgary, AB T2N 1N4, Canada
*
Author to whom correspondence should be addressed.
These authors contributed equally to this study.
Genes 2018, 9(7), 344; https://doi.org/10.3390/genes9070344
Received: 7 June 2018 / Revised: 30 June 2018 / Accepted: 3 July 2018 / Published: 6 July 2018
(This article belongs to the Special Issue Genomics of Bacterial Metal Resistance)
It is essential to understand the mechanisms by which a toxicant is capable of poisoning the bacterial cell. The mechanism of action of many biocides and toxins, including numerous ubiquitous compounds, is not fully understood. For example, despite the widespread clinical and commercial use of silver (Ag), the mechanisms describing how this metal poisons bacterial cells remains incomplete. To advance our understanding surrounding the antimicrobial action of Ag, we performed a chemical genetic screen of a mutant library of Escherichia coli—the Keio collection, in order to identify Ag sensitive or resistant deletion strains. Indeed, our findings corroborate many previously established mechanisms that describe the antibacterial effects of Ag, such as the disruption of iron-sulfur clusters containing proteins and certain cellular redox enzymes. However, the data presented here demonstrates that the activity of Ag within the bacterial cell is more extensive, encompassing genes involved in cell wall maintenance, quinone metabolism and sulfur assimilation. Altogether, this study provides further insight into the antimicrobial mechanism of Ag and the physiological adaption of E. coli to this metal. View Full-Text
Keywords: silver; silver toxicity; silver resistance; Keio collection; Escherichia coli; antimicrobials silver; silver toxicity; silver resistance; Keio collection; Escherichia coli; antimicrobials
Show Figures

Figure 1

MDPI and ACS Style

Gugala, N.; Lemire, J.; Chatfield-Reed, K.; Yan, Y.; Chua, G.; Turner, R.J. Using a Chemical Genetic Screen to Enhance Our Understanding of the Antibacterial Properties of Silver. Genes 2018, 9, 344. https://doi.org/10.3390/genes9070344

AMA Style

Gugala N, Lemire J, Chatfield-Reed K, Yan Y, Chua G, Turner RJ. Using a Chemical Genetic Screen to Enhance Our Understanding of the Antibacterial Properties of Silver. Genes. 2018; 9(7):344. https://doi.org/10.3390/genes9070344

Chicago/Turabian Style

Gugala, Natalie, Joe Lemire, Kate Chatfield-Reed, Ying Yan, Gordon Chua, and Raymond J. Turner 2018. "Using a Chemical Genetic Screen to Enhance Our Understanding of the Antibacterial Properties of Silver" Genes 9, no. 7: 344. https://doi.org/10.3390/genes9070344

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop