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Genes 2018, 9(2), 110; https://doi.org/10.3390/genes9020110

Genome-Wide Analysis of the GRF Family Reveals Their Involvement in Abiotic Stress Response in Cassava

1
Tropical Agriculture and Foresty Institute, Hainan University, Haikou, Hainan, 570228, China
2
Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, 571101, China
3
College of Life Science and Technology, Huazhong University of Science and Technology (HUST), Wuhan, Hubei, 430074, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 12 January 2018 / Revised: 13 February 2018 / Accepted: 15 February 2018 / Published: 20 February 2018
(This article belongs to the Section Plant Genetics and Genomics)
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Abstract

GENERAL REGULATORY FACTOR (GRF) proteins play vital roles in the regulation of plant growth, development, and response to abiotic stress. However, little information is known for this gene family in cassava (Manihot esculenta). In this study, 15 MeGRFs were identified from the cassava genome and were clustered into the ε and the non-ε groups according to phylogenetic, conserved motif, and gene structure analyses. Transcriptomic analyses showed eleven MeGRFs with constitutively high expression in stems, leaves, and storage roots of two cassava genotypes. Expression analyses revealed that the majority of GRFs showed transcriptional changes under cold, osmotic, salt, abscisic acid (ABA), and H2O2 treatments. Six MeGRFs were found to be commonly upregulated by abiotic stress, ABA, and H2O2 treatments, which may be the converging points of multiple signaling pathways. Interaction network analysis identified 18 possible interactors of MeGRFs. Taken together, this study elucidates the transcriptional control of MeGRFs in tissue development and the responses of abiotic stress and related signaling in cassava. Some constitutively expressed, tissue-specific, and abiotic stress-responsive candidate MeGRF genes were identified for the further genetic improvement of crops. View Full-Text
Keywords: cassava; abiotic stress; gene expression; identification; GENERAL REGULATORY FACTOR (GRF) protein family cassava; abiotic stress; gene expression; identification; GENERAL REGULATORY FACTOR (GRF) protein family
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Shang, S.; Wu, C.; Huang, C.; Tie, W.; Yan, Y.; Ding, Z.; Xia, Z.; Wang, W.; Peng, M.; Tian, L.; Hu, W. Genome-Wide Analysis of the GRF Family Reveals Their Involvement in Abiotic Stress Response in Cassava. Genes 2018, 9, 110.

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