Genomic Features and Tissue Expression Profiles of the Tyrosinase Gene Family in the Chinese Soft-Shelled Turtle (Pelodiscus sinensis)
Abstract
1. Introduction
2. Materials and Methods
2.1. Genome Identification and Physicochemical Analysis of the TYR Gene Family
2.2. Chromosome Scaffold Mapping and Gene Structure Analysis of the TYR Gene Family
2.3. Phylogeny and Collinearity Analysis of the TYR Gene Family
2.4. Conserved Motifs and Structural Domain Analysis of the TYR Gene Family
2.5. Homology Alignment and Structural Analysis of the TYR Proteins
2.6. Transcription Factor Analysis
2.7. Quantitative Reverse Transcription PCR Analysis of the TYR Gene Family
2.7.1. Sample Collection and Ethical Declaration
2.7.2. RNA Extraction and Quantitative Reverse Transcription PCR
3. Results
3.1. Identification and Physicochemical Characterization of the TYR Gene Family Members
3.2. Chromosome Scaffold Mapping and Gene Structure of the TYR Gene Family Members
3.3. Phylogenetic Relationships of the TYR Gene Family Members in 14 Vertebrate Species
3.4. Collinearity Analysis of the TYR Gene Family Members
3.5. Conserved Motifs and Structural Domain Analysis of the TYR Gene Family Members
3.6. Amino Acid Sequence Alignment and Protein Structure Prediction of the TYR Gene Family Members
3.7. Transcription Factor Prediction of the TYR Gene Family Members
3.8. Expression Profiling of the TYR Gene Family Members in Four Kinds of P. sinensis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Primer Sequence (5′-3′) | Amplicon Length (bp) | Tm (°C) |
---|---|---|---|
TYR | F: GTTTACCCAGCAGCCAATGC R: TGGAAGGAGTCAACTGGGTCT | 152 | 60 |
TYRP1 | F: CTCCACACTGCTCCTTACAC R: CTATTACGGCTACACATTGACC | 176 | 58 |
DCT | F: GAGCTGCCAGTGTACAGGAAA R: TCACATAGTCTGGGTGCGTG | 194 | 60 |
Ef1a | F: ACTCGTCCAACTGACAAGCCTC R: CACGGCGAACATCTTTCACAG | 253 | 60 |
Gene | Number of Amino Acids | Molecular Weight | Theoretical pI | Instability Index | Aliphatic Index | Grand Average of Hydropathicity | Subcellular Localization |
---|---|---|---|---|---|---|---|
TYR | 529 | 60,152.08 | 5.56 | 51.51 | 74.90 | −0.289 | Plasma membrane |
TYRP1 | 535 | 60,157.45 | 5.35 | 49.75 | 76.00 | −0.331 | Plasma membrane |
DCT | 526 | 59,858.20 | 6.47 | 42.55 | 74.01 | −0.326 | Plasma membrane |
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Liu, Y.; Liu, P.; Ren, T.; Gao, Y.; Wang, Z.; Zhu, J.; Chen, C.; Ji, L.; Hong, X.; Liu, X.; et al. Genomic Features and Tissue Expression Profiles of the Tyrosinase Gene Family in the Chinese Soft-Shelled Turtle (Pelodiscus sinensis). Genes 2025, 16, 834. https://doi.org/10.3390/genes16070834
Liu Y, Liu P, Ren T, Gao Y, Wang Z, Zhu J, Chen C, Ji L, Hong X, Liu X, et al. Genomic Features and Tissue Expression Profiles of the Tyrosinase Gene Family in the Chinese Soft-Shelled Turtle (Pelodiscus sinensis). Genes. 2025; 16(7):834. https://doi.org/10.3390/genes16070834
Chicago/Turabian StyleLiu, Yanchao, Pan Liu, Tong Ren, Yang Gao, Ziman Wang, Junxian Zhu, Chen Chen, Liqin Ji, Xiaoyou Hong, Xiaoli Liu, and et al. 2025. "Genomic Features and Tissue Expression Profiles of the Tyrosinase Gene Family in the Chinese Soft-Shelled Turtle (Pelodiscus sinensis)" Genes 16, no. 7: 834. https://doi.org/10.3390/genes16070834
APA StyleLiu, Y., Liu, P., Ren, T., Gao, Y., Wang, Z., Zhu, J., Chen, C., Ji, L., Hong, X., Liu, X., Wei, C., Zhu, X., Chu, Z., & Li, W. (2025). Genomic Features and Tissue Expression Profiles of the Tyrosinase Gene Family in the Chinese Soft-Shelled Turtle (Pelodiscus sinensis). Genes, 16(7), 834. https://doi.org/10.3390/genes16070834