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Article

Expression of a Truncated Yeast Ccc1 Vacuolar Transporter Increases the Accumulation of Endogenous Iron

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Departamento de Bioquímica y Biología Molecular, Universitat de València, Doctor Moliner 50, 46100 Burjassot, Valencia, Spain
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Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Agustín Escardino 7, 46980 Paterna, Valencia, Spain
*
Authors to whom correspondence should be addressed.
Academic Editor: Paweł Lipiński
Genes 2021, 12(8), 1120; https://doi.org/10.3390/genes12081120
Received: 7 June 2021 / Revised: 12 July 2021 / Accepted: 15 July 2021 / Published: 23 July 2021
(This article belongs to the Special Issue Genetic Regulation in Iron Homeostasis)
Iron is an essential micronutrient for all eukaryotic organisms because it participates as a redox cofactor in multiple metabolic processes. Iron bioavailability is highly restricted due to the low solubility of its oxidized form, frequently leading to iron deficiency anemia. The baker’s yeast Saccharomyces cerevisiae is used as a model organism for iron homeostasis studies, but also as a food supplement and fermentative microorganism in the food industry. Yeast cells use the vacuolar Ccc1 transporter to detoxify and store excess iron in the vacuoles. Here, we modulate CCC1 expression and properties to increase iron extraction from the environment. We show that constitutive expression of full-length CCC1 is toxic, whereas deletion of its cytosolic amino-terminal (Nt) domain (NtΔCCC1) rescues this phenotype. Toxicity is exacerbated in cells lacking AFT1 transcription factor. Further characterization of NtΔCcc1 protein suggests that it is a partially functional protein. Western blot analyses indicate that deletion of Ccc1 Nt domain does not significantly alter GFP-Ccc1 protein stability. A functional full-length GFP-Ccc1 protein localized to particular regions of the vacuolar membrane, whereas GFP-NtΔCcc1 protein was evenly distributed throughout this endogenous membrane. Interestingly, expression of NtΔCCC1 increased the accumulation of endogenous iron in cells cultivated under iron-sufficient conditions, a strategy that could be used to extract iron from media that are not rich in iron. View Full-Text
Keywords: yeast; Saccharomyces cerevisiae; iron; Ccc1 yeast; Saccharomyces cerevisiae; iron; Ccc1
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MDPI and ACS Style

Sorribes-Dauden, R.; Martínez-Pastor, M.T.; Puig, S. Expression of a Truncated Yeast Ccc1 Vacuolar Transporter Increases the Accumulation of Endogenous Iron. Genes 2021, 12, 1120. https://doi.org/10.3390/genes12081120

AMA Style

Sorribes-Dauden R, Martínez-Pastor MT, Puig S. Expression of a Truncated Yeast Ccc1 Vacuolar Transporter Increases the Accumulation of Endogenous Iron. Genes. 2021; 12(8):1120. https://doi.org/10.3390/genes12081120

Chicago/Turabian Style

Sorribes-Dauden, Raquel, María T. Martínez-Pastor, and Sergi Puig. 2021. "Expression of a Truncated Yeast Ccc1 Vacuolar Transporter Increases the Accumulation of Endogenous Iron" Genes 12, no. 8: 1120. https://doi.org/10.3390/genes12081120

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