Search Results (23,215)

Fenton-based processes are widely used advanced oxidation methods that are known for degrading persistent pollutants. However, these techniques often generate significant amounts of iron-containing sludge, which poses environmental disposal challenges due to its complex composition. Furthermore, the sludge produced by the Fenton process contains a high content of Fe(III) compounds, which can serve as an iron source to stimulate dissimilatory iron reduction (DIR), enhancing the performance of anaerobic digestion. Based on the characterization results from a previous study, this work investigated the use of the ferrous precipitate generated by the electrochemical peroxidation process applied to tannery wastewater treatment as an additive to enhance volatile fatty acids (VFAs) production during dark fermentation. The performance of ferrous precipitate (R-Fe₃O₄) was compared to that of conventional magnetite (Fe₃O₄) during dark fermentation under high organic loading conditions, emphasizing their potential to enhance hydrolysis efficiency and VFAs production yields, while promoting sustainable resource recovery and reuse within a circular bioeconomy framework. The results showed that the addition of both Fe₃O₄ and R-Fe₃O₄ significantly increased the VFAs yields, with a predominance of long-chain fatty acids. The presence of CaCO₃ in the ferrous precipitate contributed to maintaining a stable pH environment, supporting microbial activity and enhancing the hydrolysis of soluble compounds. Moreover, the availability of essential micronutrients within the ferrous precipitate favored greater microbial diversity. Consequently, the addition of R-Fe₃O₄ promoted VFAs production, even at higher organic loading rates, suggesting a promising application of Fenton-based by-products as functional additives to improve the economic and environmental performance of the dark fermentation process. Full article

The oxygen evolution reaction (OER), a key half-reaction in electrochemical water splitting, is limited by sluggish multi-electron transfer kinetics, starting extensive research into efficient, low-cost nanoscale electrocatalysts, particularly those based on nickel, cobalt, and iron, as well as mixed-metal, hybrid, and heteroatom-doped carbon-based metal-free systems, as presented here. Ni- and Co-based electrocatalysts show high efficiency for alkaline OER due to optimized nanostructures, surface modifications, heterostructure design, and multi-metal doping, which enhance activity, stability, and electronic properties. Their performance relies on precise atomic-level control of structure and synergistic interactions, enabling them to approach or rival noble-metal catalysts. Iron-based electrocatalysts are also promising due to their abundance, low cost, and flexible redox chemistry, forming active iron oxyhydroxide species during operation; however, their low conductivity requires structural and electronic optimization. Beyond Fe, Ni, and Co, copper-based compounds, zeolitic imidazolate framework-derived structures, and manganese phosphide–cerium oxide composites offer enhanced oxygen vacancies, tunable structures, and strong interfacial synergy. Furthermore, heteroatom-doped carbon materials incorporating nitrogen, phosphorus, or sulfur improve catalytic activity by modifying electronic structure, creating active sites, and enhancing charge transfer. Overall, careful control of composition, structure, and electronic properties enables the development of efficient, durable, and scalable noble-metal-free catalysts for OER. Full article

Background/Objectives: Complementary medicines (CMs) are widely used in Australia, yet consumer beliefs about their safety and effectiveness often diverge from the scientific evidence. Contemporary statewide data from Victoria, particularly about these perceptions and underlying perception profiles, are limited. We therefore aimed to characterise CM use patterns and perceptions of it among Victorian adults and identify the demographic and use-related belief patterns. Methods: A cross-sectional survey was conducted in metropolitan and regional Victoria (November 2024–August 2025) among adults (≥18 years) who had used complementary medicines in the previous 12 months (N = 447). The questionnaire assessed CM use patterns, perceived effectiveness, safety, quality, perceived risk relative to prescription medicines, adverse events, and demographics. The analyses included descriptive statistics, χ² tests with multiple-comparison control, Spearman correlations, and a multivariable regression. An exploratory factor analysis (EFA) and latent class analysis (LCA) were used to identify the perception dimensions and distinct consumer profiles. Results: CM use was frequent (62.2% daily; 19.2% weekly) and often long term (>1 year, 55.0%). The most commonly used products were vitamin D (53.0%), multivitamins (39.8%), magnesium (34.5%), iron (33.8%), and vitamin C (30.0%). The perceptions were favourable: 77.3% rated CMs as effective, 90.4% as safe, and 60.3% as high quality; 78.5% perceived CMs to have lower side-effect risks than prescription medicines. Adverse events were reported by 12.3%. In the adjusted models, adults ≥ 65 years and monthly/occasional users were less likely to endorse “lower risk than prescription medicines” (aOR: 0.18; 95% CI: 0.06–0.51; aOR: 0.36, 0.18–0.72). East Asian respondents had lower odds of endorsing CM effectiveness than Caucasian/White respondents (aOR: 0.28, 0.11–0.72). Their perceived quality was higher among men (aOR: 1.73, 1.09–2.74) and adults aged 55–65 years (aOR: 3.81, 1.39–10.48). Conclusions: In this contemporary statewide Victorian sample, CM use was common and generally viewed positively, yet the comparative risk may be underestimated. Profiling perception patterns and identifying belief patterns by age, culture, and use intensity provides actionable targets for clinician/pharmacist counselling and culturally tailored education to support safer, evidence-aligned CM use. Full article

Background: Extracellular vesicles (EVs) released from skeletal muscle mediate metabolic communication via microRNAs (miRNAs). While both circadian rhythms and exercise influence metabolism, the joint modulation of the muscle-derived EV miRNA landscape by circadian rhythms and chronic exercise remains undefined, particularly under the metabolic stress of obesity. Methods: Employing a 2×2 factorial design (Phase: ZT3 vs. ZT15; Condition: sedentary vs. exercise; ZT, Zeitgeber Time), EV-enriched fractions were isolated from ex vivo quadriceps muscle (QUA) cultures of high-fat diet-fed mice following an 8-week treadmill training regimen using polymer-based precipitation, and comprehensive miRNA profiling was performed by small RNA sequencing. Results: Principal component analysis (PCA) revealed that circadian phase accounted for a greater proportion of global variance in EV miRNA profiles than exercise. Differential expression analysis identified miR-1a-3p and miR-1b-5p as upregulated across both composite phase and exercise contrasts; however, condition-specific analyses indicated that this signal was primarily driven by the sedentary-phase comparison (ZT15-sed vs. ZT3-sed), in which the miR-29 family was also prominently co-upregulated, rather than constituting independent phase and exercise effects; this phase-associated signature was absent in the corresponding exercise-condition comparison. Exploratory functional enrichment of experimentally validated targets revealed phase-preferential association with metabolic and iron–heme pathways, whereas exercise-associated miRNAs mapped to signaling, inflammatory, and transcription-related networks. Conclusions: Circadian phase was the dominant contributor to global variance in muscle-derived EV-enriched miRNA profiles in obesity, as reflected by the phase-associated separation along principal component 1 (PC1, 33.47% of total variance), with exercise introducing context-dependent adaptive modulation. This study provides a foundational basis for investigating the temporal regulation of muscle secretome dynamics under high-fat diet conditions, highlighting temporal specificity as a key dimension in EV-mediated exercise physiology research. Full article

Drifting fish aggregating devices (DFADs) are central to tropical tuna purse-seine fisheries, yet their hydrodynamic performance under realistic seas has not been adequately addressed, particularly for emerging eco-friendly designs. A three-dimensional framework based on computational fluid dynamics is developed to assess the motion response and mooring loads of full-scale DFADs comprising raft buoys, biodegradable cotton rope, and iron sinkers, using four buoy layouts (Models A to D). Unsteady Reynolds-averaged Navier–Stokes (URANS) simulations are performed with a realizable k–ε closure, volume of fluid (VOF) free-surface capturing, the Euler overlay method, dynamic overset meshes, and catenary mooring coupling. Regular waves representative of operational conditions (T = 1.40 to 2.40 s, H = 0.10 to 0.40 m) are imposed via a VOF wave-forcing technique, and mesh/time-step sensitivity analyses demonstrate the accurate reproduction of the first-order wave elevation (error < 0.8%). Surge drift per cycle and heave response amplitude operators, with the relative mooring force, are evaluated as functions of the relative wavelength (λ/L_a) and wave steepness (H/λ). The results reveal that the buoy layout exerts first-order control on DFAD dynamics, whereas short, steep waves dominate motion and line loads. The intermediate end-point sinker mass achieves a favorable balance between motion suppression and mooring load control, whereas distributing a fixed total sinker mass along the rope reduces heave response and mooring force by improving the tension redistribution and overall stability. Across all sea states, Models A and D reduced motion envelopes and mooring forces, indicating their suitability as robust, low-impact configurations. The proposed framework and design recommendations provide quantitative guidance for optimizing eco-DFAD geometry and deployment strategies, supporting safer and more sustainable DFAD-based tuna fisheries. Full article

Archaeological evidence for prehistoric placer tin mining is rare due to the ephemeral nature of the workings and the associated tools in the dynamic setting of active river systems. Here, we report an additional line of evidence for metallurgical activities at stream tin mining in Serbia at Mt. Cer and Bukulja. Rivers at these locations contain Pb-rich-glass grains, many of which are also enriched in Cu and Sn. Compositionally, the detrital grains of glass are similar to the vitreous infillings on a bleached ceramic sherd found at Spasovine, an archaeological site situated on the bank of the tin-rich Milinska River. The high-Pb-bearing (average 42 wt%) and Sn-bearing (average 0.7 wt%) composition of the glass, along with the inclusions of secondary cassiterite, indicate that the slag was derived from the refinement of leaded bronze (i.e., lead removal). Although the detrital glass slag grains lack direct archaeological context, broader archaeological observations limit their production to either the Roman or Medieval Periods. The presence of Pb-Cu-Sn metallurgical glass grains in a river at Bukulja provides the first concrete evidence of prehistoric tin mining at this locality, which demonstrates that sluicing for crushed glassy residues is a viable means to prospect for as yet undiscovered sites of ancient metallurgical activities. Full article

The heavy metal pollution linked to extractive activities has attracted broad public attention. To examine the current state of heavy metal pollution in river sediments around iron tailing zones, this study was carried out to evaluate the distribution features, potential sources, and environmental hazards of heavy metals (HMs, Cr, Cd, Ni, Cu, Zn, Pb, As, and Hg) in the surface sediments of rivers in the Xiangshan area of Ma’anshan City. Results indicated that, except for Cr, the mean heavy metal concentrations exceeded the soil background levels in Anhui’s Huaihe River Basin. Variability in metal concentrations among the sediments was moderate, exhibiting an uneven spatial distribution. Significant positive correlations were detected between various HMs in the sediments, suggesting a common pollution source. Source analysis findings revealed that the HMs primarily originate from agricultural fertilization, mining, and smelting activities. Evaluation results from both the single-factor pollution index and the Nemerow comprehensive index indicated that the upstream section of the Caishi River is severely polluted by HMs. The potential ecological risk index evaluation results demonstrated that 85% of sediment samples from sampling points achieved a high comprehensive potential ecological risk level for HMs, with Cd, Cu, and Hg identified as the key contributors. The human health risk assessment demonstrated that both adults and children are subjected to carcinogenic risks from heavy metal exposure, with children exhibiting a higher risk level. This study offers valuable insights into managing heavy metal contamination in river sediments adjacent to iron tailings regions. Full article

The purpose of this study is the exploration of the catalytic performance of a ZSM-5 zeolite produced from iron-rich fly ash, without any additional iron loading, in removing paracetamol via a heterogenous Fenton reaction. The structural and textural characterization by powder X-ray diffraction and N₂ adsorption isotherms showed that a pure ZSM-5 phase was synthesized, but lower crystallinity and textural parameters were obtained when compared with commercial ZSM-5. The XPS analysis revealed significant amounts of iron and yttrium, which enhanced the electronic properties of the samples’ surface when compared with iron-impregnated commercial ZSM-5. The catalytic reaction was followed through UV-spectroscopy and kinetic models were applied to the data; the best fit was obtained for a pseudo-first-order model. All fly ash-based zeolites showed increased paracetamol removal when compared with commercial iron-loaded ZSM-5, which may be attributed to the more disordered structure, able to accommodate large paracetamol species (dimers). On the other hand, the effect of yttrium on the electronic properties of iron sites may increase the ^●OH radical formation, thus increasing the paracetamol removal rate, despite the progressive drop on paracetamol removal upon regeneration–reuse cycles due to Fe leaching. Full article

Background: Children with β-thalassemia major (β-TM) survive longer due to advances in transfusion and chelation therapy; however, cardiovascular complications have emerged as a leading cause of long-term morbidity. Chronic hemolysis, oxidative stress, and iron overload may promote early endothelial dysfunction and premature vascular aging, yet their impact on myocardial deformation in pediatric patients remains incompletely characterized. Objectives: To evaluate subclinical myocardial dysfunction and arterial stiffness in children with β-TM and to investigate hemolysis-related changes in asymmetric dimethylarginine (ADMA) and L-arginine as biomarkers of endothelial dysfunction in relation to cardiovascular involvement. Methods: Twenty-four children with β-TM and 20 age-matched healthy controls were included. Cardiac structure and myocardial deformation were assessed by conventional echocardiography, tissue Doppler imaging, and speckle-tracking strain analysis. Arterial stiffness was evaluated using oscillometric pulse wave analysis and bilateral carotid intima–media thickness (CIMT). Serum ADMA and L-arginine levels were measured, and hemoglobin, reticulocyte count, and ferritin levels were recorded. Results: Children with β-thalassemia major demonstrated significantly increased arterial stiffness compared with controls, including higher PWV (4.61 ± 0.37 vs. 4.38 ± 0.31), AIx@75 (augmentation index at 75 bpm) (28.5 ± 8.34 vs. 22.8 ± 6.51), left CIMT [0.45 (0.39–0.51) vs. 0.41 (0.38–0.46)], and right CIMT [0.43 (0.39–0.54) vs. 0.40 (0.34–0.46)]. In addition, patients exhibited reduced global longitudinal strain (−19.3 ± 2.91 vs. −21.84 ± 1.91), prolonged isovolumetric relaxation time [53 (37–71) vs. 45 (37–55)], and elevated E/Em (8.44 ± 2.19 vs. 6.92 ± 1.10). ADMA levels were significantly higher in patients (0.54 ± 0.19 vs. 0.39 ± 0.22) and were positively associated with reticulocyte counts and inversely correlated with hemoglobin levels. In addition, both ADMA and ferritin levels were positively correlated with arterial stiffness indices and left ventricular filling pressures. Conclusions: Children with β-thalassemia major exhibit features suggestive of early cardiovascular aging, including impaired myocardial deformation, diastolic involvement, and increased arterial stiffness. The observed association between ADMA levels and markers of hemolysis, vascular stiffness, and myocardial deformation highlights the potential involvement of endothelial dysfunction in premature myocardial–vascular remodeling. These findings suggest that ADMA may serve as a promising biomarker for early cardiovascular risk in pediatric β-thalassemia major; however, further longitudinal and multi-center studies are needed to confirm its clinical utility for risk stratification. Full article

Degradable polymers, such as poly(L-lactide) (PLLA), are widely investigated for biomedical applications, including drug delivery systems and temporary implants. Their functionality can be expanded by incorporating degradable metal microparticles that may influence degradation behaviour and enable additional surface modification strategies. In this study, the feasibility of composites consisting of PLLA and biodegradable iron microparticles was investigated. Composites were fabricated by solvent casting, providing a gentle alternative to thermal processing methods, which often compromise polymer integrity. Composites were evaluated by thermogravimetric analysis, differential scanning calorimetry, scanning electron microscopy (SEM), tensile testing, dynamic mechanical analysis, and X-ray photoelectron spectroscopy (XPS). Incorporation of iron altered thermal behaviour and crystallinity of PLLA, indicating interactions between polymer matrix and dispersed metal phase that may affect degradation kinetics and material stability. While iron addition reduced Young’s modulus, tensile strength, and elongation at break, composites maintained sufficient structural integrity for potential biomedical applications. XPS and SEM confirmed the embedding of particles within the polymer matrix, enabling potential post-processing approaches. In vitro direct contact and eluate tests demonstrated good cell viability, whereas exposure to free iron particles resulted in dose- and time-dependent cytotoxic effects. Overall, the results demonstrate the feasibility of solvent-cast PLLA–iron composites for resorbable biomedical applications. Full article

In desert ecosystems, deep-rooted plants like Alhagi sparsifolia contribute not only to wind prevention and sand fixation but also to the transport of carbon into deep soil layers through their root systems. However, the sources and stabilization mechanisms of soil organic carbon (SOC) following plant carbon input remain unclear. This study investigated a dominant A. sparsifolia community at the southern edge of the Taklimakan Desert. We analyzed plant traits and the vertical distribution (0–200 cm) of SOC fractions—particulate organic carbon (POC), mineral-associated organic carbon (MAOC), and calcium/iron-bound organic carbon (Ca/Fe-OC)—along with carbon sources (microbial biomass, microbial necromass, and plant residue). As growth advanced, stem and root biomass increased, while leaf and thorn biomass remained stable. SOC and POC decreased by 5.38–29.43% with soil depth, whereas MAOC and Ca/Fe-OC increased by 32.34–48.15%. Plant residue contributed more to SOC (average 30.56%) than microbial necromass (8.28%), and both contributions increased by 9.60–167.68% with soil depth. No significant correlation was found between plant residue and SOC fractions, but a significant correlation with microbial necromass. In conclusion, although plant residues constitute the primary source of SOC in desert ecosystems, microbial necromassa exerts a stronger influence on SOC stability. Full article

Latitudinal gradients are widely recognized as a key macro-environmental driver shaping microbial biogeographic patterns; however, the spatial organization of sediment archaeal communities in estuarine ecosystems and the mechanisms underlying their assembly remain insufficiently understood. This study is based on sediment samples collected from three representative estuarine regions spanning distinct latitudes along the Chinese coastline—the North China Sea (NCS), East China Sea (ECS), and South China Sea (SCS). Based on 16S rRNA high-throughput sequencing, combined with null-model inference and molecular ecological network (MEN) analyses, we characterized latitudinal patterns in archaeal community distributions, assembly processes, and cross-regional interaction architectures. The results showed that archaeal communities exhibited obvious spatial segregation across three regions, with both community richness and network complexity increasing significantly toward lower latitudes. Nitrate (NO₃⁻), ferric iron (Fe³⁺), and dissolved oxygen (DO) were identified as key environmental factors governing archaeal community structure. Notably, archaeal community assembly processes exhibited a clear latitudinal gradient: deterministic processes, particularly environmental filtering, were more obvious at lower latitudes, whereas the contributions of stochastic processes—including dispersal limitation and ecological drift—increased markedly at higher latitudes. A MEN analysis further revealed that archaeal networks at lower latitudes exhibited higher connectivity, modularity, and stability, suggesting that interspecific interactions may enhance ecosystem resistance to environmental disturbance under more stable environmental conditions. Overall, this study demonstrates that macro-environmental gradients jointly shape archaeal biogeographic patterns via multiple pathways, including modulation of environmental filtering, dispersal dynamics, and cross-regional interactions. These findings deepened our understanding of the stable mechanisms governing the diversity and biogeographical distribution of archaea in estuarine systems. Full article

Metabolic abnormalities are frequently associated with hepatic steatosis and low-grade inflammation, yet the contributions of iron metabolism and genetic susceptibility are not fully understood. We aimed to investigate the relationship between serum ferritin, hepatic steatosis, metabolic risk clustering, and the PNPLA3 rs738409 gene variant in children. A total of 68 children aged 6–14 years underwent anthropometric, biochemical, imaging, and genetic assessment. Hepatic steatosis was present in 72.1% of participants, with fibrosis greater than F1 in 42.6%. Serum ferritin showed a strong correlation with echographic liver steatosis severity (ρ = 0.804, p < 0.001) and a moderate correlation with the number of metabolic risk components (ρ = 0.482, p < 0.001). The highest metabolic burden occurred in children with low iron and elevated ferritin. While PNPLA3 status did not independently predict ferritin levels, carriers had a significantly higher prevalence of hypertension (50.0% vs. 25.0%, p = 0.038) and a non-significant trend toward low HDL-C (65.0% vs. 42.9%, p = 0.070). Ferritin was associated with metabolic clustering and ultrasound-defined hepatic steatosis, acting as a nonspecific marker of combined metabolic and hepatic alterations. PNPLA3 genotype was not independently related to ferritin or fibrosis in early pediatric disease. Given the cross-sectional design and the relatively small sample size, these findings should be interpreted as exploratory and further studies including larger populations and direct inflammatory markers should be conducted. Full article

Enriching bakery products with highly nutritious ingredients, such as microalgae and insect powder, is a promising strategy for developing functional foods. This study aimed to evaluate the effects of spirulina, chlorella, and cricket powder on the quality of sponge cakes. The assessed parameters included color, nutritional value, mineral composition, antioxidant activity, predicted glycemic index (pGI), and sensory properties. The addition of microalgae significantly reduced the L* value and altered the color shade of the sponge cakes, while the insect powder caused milder color changes. The enriched samples contained higher levels of protein (by up to 14%) and minerals, including calcium, magnesium, iron, and zinc. Antioxidant activity was enhanced across all variations, particularly in sponge cakes with insect powder, which showed the highest TPC (47.96 mg GAE), DPPH· (0.107 mM TE), and ABTS·⁺ (0.208 mM TE) levels. Cakes containing spirulina exhibited the highest total flavonoid content (63.95 mg EPI). Additionally, the enriched samples demonstrated a statistically significant reduction in the pGI. Among all the supplemented samples, the sponge cake with cricket powder received the highest consumer acceptance. Overall, enriching sponge cakes with microalgae and cricket powder improved their nutritional value and antioxidant properties, with insect powder offering the best balance between sensory quality and functionality. Full article

Uveal melanoma (UM) represents an uncommon intraocular malignancy with high aggressiveness. Dysregulation of ferroptosis has been associated with UM progression. Dihydroartemisinin (DHA), a natural derivative of Artemisia annua, exhibits potent antitumor activity with a favorable safety profile, yet its role in ferroptosis regulation in UM remains unclear. Here, we showed that DHA significantly reduced the proliferation and invasiveness of UM cells—both primary and secondary—with effects intensifying over time and dose. Transcriptomic analysis indicated that DHA may exert antitumor effects by modulating the ferroptosis-related pathway, characterized by upregulating heme oxygenase-1 (HO-1) and downregulating the SLC7A11 (xCT)/GPX4 axis, leading to iron accumulation, increased ROS and lipid peroxidation, and mitochondrial dysfunction. Iron chelators and pancaspase inhibitors partially reverse these effects, whereas HO-1 inducers enhance them. Overall, our results suggest that DHA suppresses UM progression by inducing ferroptosis and mitochondrial dysfunction, while the HO-1 and xCT/GPX4 pathways may contribute to these effects. DHA may represent a potential therapeutic approach for UM, warranting further investigation. Full article