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Article

The Seed Development Factors TT2 and MYB5 Regulate Heat Stress Response in Arabidopsis

1
Institute of Plant Sciences Paris-Saclay, Université Paris-Saclay, Univ. Evry, INRAE, CNRS, 91405 Orsay, France
2
Institut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, 78000 Versailles, France
3
Bioscience and Laboratory of Molecular Biology, Wageningen University and Research, 6708PB Wageningen, The Netherlands
4
Darwin21, Biological and Environmental Science and Engineering Division (BESE), King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia
5
Max Perutz Laboratories, University of Vienna, 1030 Vienna, Austria
*
Author to whom correspondence should be addressed.
Academic Editor: Patrizia Galeffi
Genes 2021, 12(5), 746; https://doi.org/10.3390/genes12050746
Received: 6 April 2021 / Revised: 30 April 2021 / Accepted: 10 May 2021 / Published: 15 May 2021
(This article belongs to the Special Issue Genetics and Evolution of Abiotic Stress Tolerance in Plants)
HEAT SHOCK FACTOR A2 (HSFA2) is a regulator of multiple environmental stress responses required for stress acclimation. We analyzed HSFA2 co-regulated genes and identified 43 genes strongly co-regulated with HSFA2 during multiple stresses. Motif enrichment analysis revealed an over-representation of the site II element (SIIE) in the promoters of these genes. In a yeast 1-hybrid screen with the SIIE, we identified the closely related R2R3-MYB transcription factors TT2 and MYB5. We found overexpression of MYB5 or TT2 rendered plants heat stress tolerant. In contrast, tt2, myb5, and tt2/myb5 loss of function mutants showed heat stress hypersensitivity. Transient expression assays confirmed that MYB5 and TT2 can regulate the HSFA2 promoter together with the other members of the MBW complex, TT8 and TRANSPARENT TESTA GLABRA 1 (TTG1) and that the SIIE was involved in this regulation. Transcriptomic analysis revealed that TT2/MYB5 target promoters were enriched in SIIE. Overall, we report a new function of TT2 and MYB5 in stress resistance and a role in SIIE-mediated HSFA2 regulation. View Full-Text
Keywords: environmental stress; seed development; site II element; HSFA2; TT2/ MYB5-MBW complex environmental stress; seed development; site II element; HSFA2; TT2/ MYB5-MBW complex
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MDPI and ACS Style

Jacob, P.; Brisou, G.; Dalmais, M.; Thévenin, J.; van der Wal, F.; Latrasse, D.; Suresh Devani, R.; Benhamed, M.; Dubreucq, B.; Boualem, A.; Lepiniec, L.; Immink, R.G.H.; Hirt, H.; Bendahmane, A. The Seed Development Factors TT2 and MYB5 Regulate Heat Stress Response in Arabidopsis. Genes 2021, 12, 746. https://doi.org/10.3390/genes12050746

AMA Style

Jacob P, Brisou G, Dalmais M, Thévenin J, van der Wal F, Latrasse D, Suresh Devani R, Benhamed M, Dubreucq B, Boualem A, Lepiniec L, Immink RGH, Hirt H, Bendahmane A. The Seed Development Factors TT2 and MYB5 Regulate Heat Stress Response in Arabidopsis. Genes. 2021; 12(5):746. https://doi.org/10.3390/genes12050746

Chicago/Turabian Style

Jacob, Pierre, Gwilherm Brisou, Marion Dalmais, Johanne Thévenin, Froukje van der Wal, David Latrasse, Ravi Suresh Devani, Moussa Benhamed, Bertrand Dubreucq, Adnane Boualem, Loic Lepiniec, Richard G.H. Immink, Heribert Hirt, and Abdelhafid Bendahmane. 2021. "The Seed Development Factors TT2 and MYB5 Regulate Heat Stress Response in Arabidopsis" Genes 12, no. 5: 746. https://doi.org/10.3390/genes12050746

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