Search Results (7,354)

In the process of maize production, extreme meteorological conditions such as drought and high temperature are often the main environmental stress factors affecting pollination efficiency. Previous studies have shown that, under adversity, the germination rate of pollen grains on the filaments of female spikes directly affects the success rate of reproduction and ultimately determines the grain yield. This study focuses on a cysteine protease named ZmPCP. The expression of this protease in maize pollen is significantly higher than in other tissues, and its specific function has not been clearly defined. Its localization in the cell membrane or apoplast was further confirmed by transient transfection experiments and plasmolysis. The interaction between ZmPCP and ZmSNAP33 was verified by yeast two-hybrid technology and a GST pull-down experiment, indicating that ZmPCP may affect pollen germination and stress resistance by regulating vesicle transport. Secondly, by analyzing the pollen germination rate of maize inbred lines B104, ZmPCP-KO and ZmPCP-OE transgenic maize plants, we found that ZmPCP overexpression could significantly enhance pollen viability and pollen tube growth under drought stress. After 1 h of short-term drying treatment, the pollen germination rate of the ZmPCP-OE line was maintained at 44%, which was significantly higher than that of the other lines. In addition, the observation of pollen tube growth showed that ZmPCP overexpression could promote the extension of pollen tubes in the filament. Moreover, a transcriptome sequencing analysis revealed the regulatory effects of ZmPCP on pollen in multiple biological processes, including stress response, carbohydrate metabolism, growth and development, cell wall material metabolism, signal transduction, etc. The involved pathways of these differential genes indicate that ZmPCP enhances pollen drought tolerance and promotes pollen tube growth through a “metabolism signal structure”. In the germination experiment on the seventh day, the germination rate of ZmPCP-OE maize seeds was the lowest, indicating that its overexpression inhibited seed germination. At the same time, ZmPCP-overexpressing Arabidopsis showed a significant advantage in taproot growth under high-concentration ABA stress. ZmPCP provides an important theoretical basis for regulating the pollination process and improving the pollination efficiency of maize varieties through interaction with ZmSNAP33. Full article

Drought is one of the major constraints to lentil production worldwide, making the development of drought-tolerant varieties essential for stable yields. Identifying genes and markers linked to drought tolerance is a crucial first step. We analyzed 90 recombinant inbred lines (RILs) derived from an interspecific cross between the drought-susceptible Lens culinaris cv. Alpo and the tolerant L. odemensis ILWL235 to investigate genomic regions associated with drought tolerance. Using 4163 high-quality SNP markers obtained through Genotyping-by-Sequencing (GBS), we constructed a linkage map showing seven groups corresponding to the lentil chromosomes. The map spans 786.82 cM and covers 3.46 G bp, representing approximately 88% of the lentil genome. To assess drought tolerance, RILs were subjected to water stress under greenhouse conditions by maintaining the soil moisture at a 40% field capacity (FC) in pots for 15 days, with the leaf relative water content (RWC) recorded every two days. Plants were phenotyped for yield, 100-seed weight, and seed number under both control and stress conditions. We identified 26 Quantitative Trait Loci (QTLs) strongly associated with drought tolerance traits and found putative candidate genes for most of them. Additional traits, including stem pigmentation, flower coloration, seed coat patterning, and seed ground color, were also mapped, and their genomic locations validated the accuracy of our linkage map. Full article

In the current context of environmental sustainability and reduced agricultural inputs, biostimulants represent one of the most efficient, eco-friendly and innovative strategies to preserve plants from biotic and abiotic stresses and to ensure sustainable agriculture. Ranging from benefic microorganisms, seaweed extracts, and humic acids to complex carbohydrates such as polysaccharides and oligosaccharides, these biostimulants are able to increase plant growth, photosynthetic efficiency, root development and nutrient uptake when they are applied during seed priming as foliar sprays or as liquid and solid soil amendments. The mechanisms underlying their effective action on plants are mainly related to the enhancement of antioxidant defenses and the regulation of hormonal pathways, particularly auxin homeostasis and transport. Several studies reported the relevance of biostimulant application in promoting root growth. In plants, roots play crucial roles, performing a variety of functions such as nutrients and water uptake, mediating stress perception and adaptation, influencing the rhizosphere microbiome, and providing structural support. The effectiveness and perception of polysaccharide-based biostimulants (PBs) are highly dependent on crucial factors, including the degree of depolymerization and the chemical modifications such as acetylation, methylation, sulfation, and oxidation. Furthermore, not all receptors and co-receptors involved in the recognition of PBs have yet been identified. However, there remain many gaps in our understanding regarding the interaction between biostimulants and roots, which is still far from fully elucidated. For these reasons, the present review provides a comprehensive overview of current research on biostimulants–root interactions, with a particular focus on polysaccharide-based biostimulants. It highlights the mechanisms involved in their recognition by plants roots, from perception to response, and the subsequent signaling cascades and the molecular pathways activated, with special emphasis on existing knowledge gaps and future research perspectives. Full article

As core equipment for precision agriculture, precision seeders directly impact the sustainability and economic efficiency of modern agriculture. With the increasing demands for precision farming and high-speed operation, precision seeders featuring state-of-the-art mechanical designs, multi-source data fusion, and flexible system expansion have become the current research trend. This paper reviews the research status and development trends, focusing on advanced mechanical design, precise control, and adjustability: (1) innovative mechanical structures, including seeding unit, driving system, and sensing system of precision seeders; (2) precision control technologies, including seed spacing, seeding depth, trajectory control, and monitoring; (3) flexible system extensibility, such as multi-crop adaptability and multi-functional integration. After systematically reviewing and analyzing these technologies, this paper further discusses the current development status, identifies existing challenges, and explores future trends in precision seeders, offering valuable insights for the advancement of precision seeders and the intelligent transformation of agriculture. Full article

Polysaccharides extracted from Japanese pumpkin (Cucurbita maxima Duchesne) possess antioxidant activity and moisturizing effects. To meet the demand for natural skincare, this study aims to develop ultra-micro liquid crystal (ULC) emulsions containing pumpkin seed oil (PO) and Japanese pumpkin polysaccharide (PP). The novelty lies in the synergistic triple-action mechanism of the lipid lamellar structure, emollients and humectants, which together achieve superior moisturization. The formulation is varied by different emulsifiers (Emulgade^® PL 68/50 and Olivem^® 1000), thickening agents (0.3–0.5% w/w of hydroxyethyl cellulose, xanthan gum, or guar gum), and active concentrations of 2.0–4.0% w/w PO and 0.1% w/w PP. Physicochemical characterization was conducted via polarized light microscopy, particle size analysis, and wide-angle X-ray diffraction (WAXD). Stability was assessed through centrifugation and six heating–cooling cycles, while clinical safety and moisturizing efficacy were evaluated in human volunteers using the Corneometer^® and Tewameter^®. Polarized light microscopy revealed distinct Maltese cross structures, while WAXD confirmed the presence of α-gel and lamellar (Lα) phases. The ULC emulsion containing PO and PP (F9), comprising 4.5% Emulgade^® PL 68/50, 0.3% xanthan gum, 2.0% PO, and 0.1% PP, demonstrated excellent physical stability and a particle size of 4.02 ± 0.02 µm. Clinical results demonstrated that F9 was non-irritating and significantly enhanced skin hydration, while reducing transepidermal water loss compared to the baseline (p < 0.05). Although F9 showed the greatest numerical improvement in barrier function, its efficacy was comparable to placebo cream and ULC emulsion containing PO (F6) (p > 0.05). In conclusion, the successful integration of pumpkin-derived actives into a stable ULC system provides a safe and effective approach for advanced moisturizing skincare applications. Full article

Sustainability is a major challenge for global food systems, particularly in the context of food loss and waste. Approximately one-third of food produced worldwide (1.3 billion tons annually) is lost or wasted, contributing to 8–10% of global greenhouse gas emissions, with a large share occurring during post-harvest handling and food processing. These stages generate by-products such as shells, skins, pulp, stems, and seeds, which can account for 30–50% of raw materials. Although often discarded, these residues are rich in valuable bioactive compounds, including phenolics, peptides, carotenoids, fibers, secondary metabolites, minerals, amino acids, and vitamins. This review emphasizes the valorization of agrifood by-products as a pathway toward sustainability and the achievement of the Sustainable Development Goals (SDGs). It encompasses extraction methods, characterization, and potential uses of such active compounds in the food, pharmaceutical, packaging, and cosmetic sectors. Moreover, it examines the interaction between valuing agrifood by-products and key SDGs like eliminating hunger (SDG 2), ensuring good health and well-being (SDG 3), promoting affordable and clean energy (SDG 7), promoting economic growth and decent work (SDG 8), ensuring responsible consumption and production (SDG 12), and tackling climate action (SDG 13). These approaches have high potential to improve food security and economic sustainability of the world’s food systems. Full article

Domestically developed wheat breeding plot combine harvesters in China currently utilize cyclone separation self-cleaning systems. However, these systems struggle to meet the agronomic requirement of zero wheat grain residue. Seed mixing caused by residual grains can compromise the accuracy of entire breeding field trials. This study focused on the structural design of a cyclone separation self-cleaning system based on high self-cleaning agronomic requirements. Research was conducted on the key structural and operational parameters of the cyclone separator and the negative-pressure centrifugal fan, preliminarily determining the ranges for critical parameters such as the diameter of the cylindrical section of the separator wall, the dust outlet diameter, and the rotational speed of the negative-pressure centrifugal fan. A test bench for the cyclone separation self-cleaning system of wheat breeding plot combine harvesters was designed and developed. Through single-factor experiments and Box–Behnken design optimization, the effects of key parameters on system performance were investigated. The optimal parameter combination—cylindrical section diameter of 614 mm, dust outlet diameter of 290 mm, and fan speed of 1495 r/min—achieved a self-cleaning rate of 100%, self-cleaning time ≤12 s, loss rate of 1.70%, and impurity rate of 0.16%, fully meeting the requirements for high-quality, rapid, and effective separation and self-cleaning operations. Full article

Despite advances in the treatment of cutaneous melanoma, there is still a high percentage of patients who fail to respond or develop resistance to treatment. Establishing robust in vitro melanoma models will enable mechanism-based drug screening while reducing animal testing. In this work, a three-dimensional (3D) melanoma skin model (3DMSM) was developed on a porous scaffold. The culture of three melanoma cell lines (SKMEL-1, A375, and G361) in co-culture with human fibroblasts, melanocytes, and keratinocytes allowed the formation of the dermis, and stratified epidermis. Tumors were established in this model using two methodologies: adding previously formed melanoma cell aggregates (CA) or seeding melanoma cells directly into the dermis (CD). In this model, melanoma cells remain in their original microenvironment and, after proliferation, invade the basal layer. The model recapitulates correct melanocyte localization, epidermal disruption, extracellular matrix (ECM) remodeling, including collagen deposition, and epithelial-to-mesenchymal transition (EMT). Additionally, the cytokine profiles studied indicate that the model could mirror the inflammatory and immune-evasive traits of melanoma. Overall, 3DMSM provides a useful tool for understanding the mechanisms of melanoma progression and invasion, and for developing personalized medicine strategies through the implementation of a patient-derived model. Full article

Taxus cuspidata is a threatened subalpine conifer in South Korea, necessitating evidence-based restoration strategies to counter the impacts of climate change. In this study, we assessed 13 natural populations using 15 polymorphic nuclear simple sequence repeat (nSSR) markers developed in Taxus species and spatial autocorrelation analysis to provide a scientific foundation for conservation. The results showed an intermediate level of genetic diversity, with the Mt. Gariwangsan population exhibiting higher diversity. This highlights its priority as a source for restoration materials. Bayesian clustering supported four distinct management units. Spatial autocorrelation analysis revealed significant positive genetic structure within approximately 50 m, indicating a localized genetic patch size. Based on these results, we suggest maintaining a minimum 50 m sampling distance during seed collection to avoid collecting closely related individuals and to reduce the risk of genetic homogeneity in restoration materials. Such restoration strategies informed by spatial genetic structure and broader genetic data are critical for enhancing the long-term resilience of T. cuspidata in the face of accelerating environmental shifts. Full article

YUCCA belongs to the flavin-containing monooxygenas and catalyzes the rate-limiting step in endogenous auxin biosynthesis, thereby regulating local auxin homeostasis and participating in diverse aspects of plant growth, development, and physiological processes. However, the relationship between the YUCCA genes and male fertility regulation in wheat remains unclear. In this study, we identified 64 TaYUCCA genes through whole-genome analysis and classified them into three clades, each of which is conserved in motif composition and gene structure. A synteny analysis indicated that family expansion was primarily driven by segmental duplication and tandem duplication, and Ka/Ks analysis suggested that all members are under purifying selection. An analysis of the expression patterns showed that the TaYUCCA genes displayed differential expression across various tissues and reproductive developmental stages. In the temperature-sensitive male-sterile wheat line YS3038, TaYUCCA19, TaYUCCA22, and TaYUCCA25 were specifically highly expressed at the uninucleate pollen stage under fertile conditions. The silencing of TaYUCCA19 resulted in abnormal pollen morphology and a significant reduction in the seed set rate, indicating that it is a key gene required for normal pollen development in wheat. Overall, this study systematically characterizes the wheat YUCCA gene family and provides the first functional evidence of TaYUCCA genes in male reproductive development, offering an important foundation for studies on wheat male sterility mechanisms and the exploitation of heterosis. Full article

Water scarcity and increasing climate variability have strengthened the demand for effective weather-modification technologies such as cloud seeding. In Thailand, conventional manned rainmaking aircraft remain constrained by operational range, safety risks, and sustainability considerations, motivating the development of electric vertical take-off and landing unmanned aerial vehicles (eVTOL-UAVs). This paper proposes a mission-driven conceptual design and optimization framework for a cloud-seeding eVTOL-UAV, and extends it to reliability-based design optimization (RBDO) under uncertainty. The design task is formulated as a five-objective many-objective optimization problem with the following objectives: minimizing take-off weight, turn radius, and probability of failure, while maximizing endurance and climb rate, subject to stability/control and performance constraints. Ten state-of-the-art many-objective metaheuristics are benchmarked and solve the problem, and their performance is assessed using hypervolume (HV), inverted generational distance (IGD), runtime, and Friedman rank statistics. Results show that AGEMOEAII and PREA consistently provide the most competitive solution-set quality (HV/IGD) with comparable computational cost across algorithms. A deterministic–reliability comparison further demonstrates a clear robustness gap. Five representative Pareto designs from the best-performing optimizer are reported to illustrate practical trade-offs and support decision-making for sustainable, autonomous cloud-seeding operations. Full article

Korean pine (Pinus koraiensis) is a vital woody oil tree species native to Northeast Asia, with its pine nuts serving as the primary global source of edible pine nuts globally due to their rich nutritional content. Currently, seed yield from Korean pine is low and unstable, failing to meet the market demand. The limited number of female cones is the primary factor restricting its yield. MADS-box family members are crucial in regulating the initiation, differentiation, and morphogenesis of floral organs. However, systematic identification and characterization of MADS-box proteins in Korean pine have not been reported. This study utilized transcriptome data from reproductive and vegetative buds during the flower bud differentiation stage of Korean pine to comprehensively identify MADS-box family members through bioinformatics analysis and molecular biology approaches. A total of 37 PkMADS-box genes were identified, including 6 type I and 31 type II (MIKC) genes, which were classified into 8 subfamilies. The physicochemical properties, conserved domains, conserved motifs, protein structures, gene expression profiles, and protein–protein interaction networks of these genes were analyzed. Key genes associated with physiological differentiation (flower induction) and sexual organogenesis were identified based on expression patterns during flower bud differentiation and flower organ development. Among these, PkMADS4 and PkMADS26 are likely involved in positively regulating flower induction, while PkMADS9 plays a role in the morphological differentiation of sexual organs in a dose-dependent manner and overexpression of PkMADS9 promoting early flowering in transgenic Arabidopsis. These genes were also identified as key candidates for regulating reproductive phase changes and strobilus development. This study provides a theoretical foundation for further ‌investigation of MADS-box genes in reproduction and offers insights into genetic improvements aimed at enhancing the seed yield of Korean pine. Full article

Potato virus X (PVX) and potato virus Y (PVY) are major pathogens that threaten seed potato quality and yield. To improve the efficiency of field screening, we developed monovalent PVX, monovalent PVY, and bivalent PVX/PVY nanozyme strips using Fe₃O₄ nanozymes as labels in a double-antibody sandwich lateral flow immunochromatographic assay. Western blot analysis demonstrated that four monoclonal antibodies (PVX 2, PVX 6, PVY 2, and PVY 5) specifically recognized their corresponding viral coat proteins. Specificity testing showed that the nanozyme strips reacted only with the target viruses and did not cross-react with other common potato viruses, including Potato virus A (PVA), Potato virus M (PVM), Potato virus S (PVS), and Potato leafroll virus (PLRV). The PVX nanozyme strip detected PVX-positive extracts diluted up to 10³-fold, the PVY nanozyme strip up to 10⁴-fold, and the bivalent strip detected PVX/PVY co-infected samples diluted up to 10³-fold. In addition, detection results by strips from 12 samples of plantlets in vitro were fully consistent with RT-PCR. These nanozyme strips provide rapid, simple, specific, and sensitive methods that can be stored at ambient temperature, enabling field surveys, warehouse screening, and on-site testing and supporting early detection of potato virus diseases. Full article

Temperate grain legumes, including faba bean, field pea, chickpea, lentil, and grass pea, are important food and forage crops in the cereal-based cropping system in the Nile Valley and Red Sea region countries. Despite their importance, local production remains insufficient, and the countries are forced to import to narrow the demand gaps. Emerging diseases, such as faba bean gall disease and several viruses (Chickpea chlorotic dwarf virus, Chickpea chlorotic stunt virus, Faba bean necrotic yellows virus, and Pea seed-borne mosaic virus), are on the rise due to climate variability, changes in farming systems such as monocropping, reduced crop rotations, limited knowledge about the pathogens, and absence of varieties with good levels of resistance. This review synthesizes research achievements in the region and identifies focus areas, primarily resistance breeding, characterization of pathogen populations, developing efficient screening techniques, investigations of mixed virus infections, advancement of pathogen diagnostic techniques, and developing agroecologically based disease management strategies to reduce economic impacts of new and re-emerging diseases. Moreover, research collaboration and information exchange among countries in the region are essential to mitigate the growing threat of emerging legume diseases. Full article

Osteosarcoma is one of the most common malignant tumors that develop in the bone. Currently, surgery is often the best and most used approach, often preceded and followed by chemotherapy, which, however, carries serious short- and long-term side effects. Recently, much attention has been paid to natural compounds capable of inducing tumor cell death, reducing tumor and metastatic activity, and interacting with selective chemotherapy targeting tumor cells. Griffonia simplicifolia, a tropical African plant, has attracted attention because its extracts with bioactive chemicals have demonstrated multiple therapeutic uses. We show the antitumor properties of a Griffonia seed extract, obtained by maceration in a hydroalcoholic mixture (ethanol/water, 70/30, v/v, Gri70), on osteosarcoma cell lines, evaluating cytotoxicity, interaction with a pro-inflammatory signal (interleukin-1β), epigenetic activity of this signal on interleukin-6 gene expression, and interactions with an elective chemotherapeutic agent, doxorubicin. Although the extract did not have strong antiproliferative activity in the cell lines analyzed, we nevertheless observed that it was able to block proliferative and migration signals induced by interleukin-1β, as well as acting epigenetically by blocking the de-methylation of the interleukin-6 promoter and its expression. Furthermore, the extract did not appear to interfere with the antitumor activity of doxorubicin, and the interaction potentiated antimetastatic effects. These results indicate that Gri-70 extract may be useful as adjuvants to enhance the effect of doxorubicin, reducing the adverse effects associated with the increased EMT process of osteosarcoma cells that manage to overcome cell death induction. Indeed, metastasis represents the main cause of poor prognosis. Full article