Next Article in Journal
Whole Exome Sequencing Reveals a Novel AUTS2 In-Frame Deletion in a Boy with Global Developmental Delay, Absent Speech, Dysmorphic Features, and Cerebral Anomalies
Next Article in Special Issue
Congruent Genetic and Demographic Dispersal Rates in a Natural Metapopulation at Equilibrium
Previous Article in Journal
TEfinder: A Bioinformatics Pipeline for Detecting New Transposable Element Insertion Events in Next-Generation Sequencing Data
Previous Article in Special Issue
Patterns of Epigenetic Diversity in Two Sympatric Fish Species: Genetic vs. Environmental Determinants
Article

Phenotypic Response to Light Versus Shade Associated with DNA Methylation Changes in Snapdragon Plants (Antirrhinum majus)

1
PSL Université Paris: EPHE-UPVD-CNRS, USR 3278 CRIOBE, Université de Perpignan, 52 Avenue Paul Alduy, CEDEX 9, 66860 Perpignan, France
2
Université Montpellier, CNRS, IFREMER, UPVD, Interactions Hôtes Pathogènes Environnements (IHPE), 66860 Perpignan, France
3
Laboratoire Évolution & Diversité Biologique (EDB, UMR 5174), Université Fédérale de Toulouse Midi-Pyrénées, CNRS, IRD, UPS, 118 route de Narbonne, Bat 4R1, CEDEX 9, 31062 Toulouse, France
*
Author to whom correspondence should be addressed.
These authors share first authorship of this work.
Academic Editors: Delphine Legrand and Simon Blanchet
Genes 2021, 12(2), 227; https://doi.org/10.3390/genes12020227
Received: 30 December 2020 / Revised: 27 January 2021 / Accepted: 27 January 2021 / Published: 4 February 2021
(This article belongs to the Special Issue Evolutionary Dynamics of Wild Populations)
The phenotypic plasticity of plants in response to change in their light environment, and in particularly, to shade is a schoolbook example of ecologically relevant phenotypic plasticity with evolutionary adaptive implications. Epigenetic variation is known to potentially underlie plant phenotypic plasticity. Yet, little is known about its role in ecologically and evolutionary relevant mechanisms shaping the diversity of plant populations in nature. Here we used a reference-free reduced representation bisulfite sequencing method for non-model organisms (epiGBS) to investigate changes in DNA methylation patterns across the genome in snapdragon plants (Antirrhinum majus L.). We exposed plants to sunlight versus artificially induced shade in four highly inbred lines to exclude genetic confounding effects. Our results showed that phenotypic plasticity in response to light versus shade shaped vegetative traits. They also showed that DNA methylation patterns were modified under light versus shade, with a trend towards global effects over the genome but with large effects found on a restricted portion. We also detected the existence of a correlation between phenotypic and epigenetic variation that neither supported nor rejected its potential role in plasticity. While our findings imply epigenetic changes in response to light versus shade environments in snapdragon plants, whether these changes are directly involved in the phenotypic plastic response of plants remains to be investigated. Our approach contributed to this new finding but illustrates the limits in terms of sample size and statistical power of population epigenetic approaches in non-model organisms. Pushing this boundary will be necessary before the relationship between environmentally induced epigenetic changes and phenotypic plasticity is clarified for ecologically relevant mechanisms with evolutionary implications. View Full-Text
Keywords: phenotypic plasticity; epigenetics; epiGBS; stem elongation; shade avoidance phenotypic plasticity; epigenetics; epiGBS; stem elongation; shade avoidance
Show Figures

Figure 1

MDPI and ACS Style

Mouginot, P.; Luviano Aparicio, N.; Gourcilleau, D.; Latutrie, M.; Marin, S.; Hemptinne, J.-L.; Grunau, C.; Pujol, B. Phenotypic Response to Light Versus Shade Associated with DNA Methylation Changes in Snapdragon Plants (Antirrhinum majus). Genes 2021, 12, 227. https://doi.org/10.3390/genes12020227

AMA Style

Mouginot P, Luviano Aparicio N, Gourcilleau D, Latutrie M, Marin S, Hemptinne J-L, Grunau C, Pujol B. Phenotypic Response to Light Versus Shade Associated with DNA Methylation Changes in Snapdragon Plants (Antirrhinum majus). Genes. 2021; 12(2):227. https://doi.org/10.3390/genes12020227

Chicago/Turabian Style

Mouginot, Pierick, Nelia Luviano Aparicio, Delphine Gourcilleau, Mathieu Latutrie, Sara Marin, Jean-Louis Hemptinne, Christoph Grunau, and Benoit Pujol. 2021. "Phenotypic Response to Light Versus Shade Associated with DNA Methylation Changes in Snapdragon Plants (Antirrhinum majus)" Genes 12, no. 2: 227. https://doi.org/10.3390/genes12020227

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop