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Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source

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Programa de Doctorado en Ciencias de Recursos Naturales, Universidad de La Frontera, 4780000 Temuco, Chile
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Centro de Excelencia en Investigación Biotecnológica Aplicada al Medio Ambiente (CIBAMA), Facultad de Ingeniería y Ciencias, Universidad de La Frontera, 4780000 Temuco, Chile
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BioNanotechnology and Microbiology Lab, Center for Bioinformatics and Integrative Biology, Facultad de Ciencias de la Vida, Universidad Andres Bello, 8320000 Santiago, Chile
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Laboratorio de Microbiología Aplicada y Extremófilos, Facultad de Ingeniería y Ciencias Geológicas, Universidad Católica del Norte, 1240000 Antofagasta, Chile
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Departamento de Ciencias Químicas y Recursos Naturales, Facultad de Ingeniería y Ciencias, Universidad de La Frontera, 4780000 Temuco, Chile
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Authors to whom correspondence should be addressed.
Academic Editor: Tino Polen
Genes 2021, 12(2), 187; https://doi.org/10.3390/genes12020187
Received: 26 December 2020 / Revised: 20 January 2021 / Accepted: 22 January 2021 / Published: 27 January 2021
(This article belongs to the Section Microbial Genetics and Genomics)
Here, we present the draft genome sequence of Pseudomonas sp. GC01, a cadmium-resistant Antarctic bacterium capable of biosynthesizing CdS fluorescent nanoparticles (quantum dots, QDs) employing a unique mechanism involving the production of methanethiol (MeSH) from methionine (Met). To explore the molecular/metabolic components involved in QDs biosynthesis, we conducted a comparative genomic analysis, searching for the genes related to cadmium resistance and sulfur metabolic pathways. The genome of Pseudomonas sp. GC01 has a 4,706,645 bp size with a 58.61% G+C content. Pseudomonas sp. GC01 possesses five genes related to cadmium transport/resistance, with three P-type ATPases (cadA, zntA, and pbrA) involved in Cd-secretion that could contribute to the extracellular biosynthesis of CdS QDs. Furthermore, it exhibits genes involved in sulfate assimilation, cysteine/methionine synthesis, and volatile sulfur compounds catabolic pathways. Regarding MeSH production from Met, Pseudomonas sp. GC01 lacks the genes E4.4.1.11 and megL for MeSH generation. Interestingly, despite the absence of these genes, Pseudomonas sp. GC01 produces high levels of MeSH. This is probably associated with the metC gene that also produces MeSH from Met in bacteria. This work is the first report of the potential genes involved in Cd resistance, sulfur metabolism, and the process of MeSH-dependent CdS QDs bioproduction in Pseudomonas spp. strains. View Full-Text
Keywords: Antarctic bacteria; nanoparticle biosynthesis; comparative genomics; volatile sulfur compounds Antarctic bacteria; nanoparticle biosynthesis; comparative genomics; volatile sulfur compounds
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MDPI and ACS Style

Gallardo-Benavente, C.; Campo-Giraldo, J.L.; Castro-Severyn, J.; Quiroz, A.; Pérez-Donoso, J.M. Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source. Genes 2021, 12, 187. https://doi.org/10.3390/genes12020187

AMA Style

Gallardo-Benavente C, Campo-Giraldo JL, Castro-Severyn J, Quiroz A, Pérez-Donoso JM. Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source. Genes. 2021; 12(2):187. https://doi.org/10.3390/genes12020187

Chicago/Turabian Style

Gallardo-Benavente, Carla; Campo-Giraldo, Jessica L.; Castro-Severyn, Juan; Quiroz, Andrés; Pérez-Donoso, José M. 2021. "Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source" Genes 12, no. 2: 187. https://doi.org/10.3390/genes12020187

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