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Article

An Integrative Transcriptomic and Metabolomic Analysis of Red Pitaya (Hylocereus polyrhizus) Seedlings in Response to Heat Stress

1
Guangdong Provincial Key Laboratory of Applied Botany & Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
2
University of Chinese Academy of Sciences, Beijing 100049, China
3
Wenshan Academy of Agricultural Sciences, Wenshan 663000, China
4
CAS Engineering Laboratory for Vegetation Ecosystem Restoration on Islands and Coastal Zones, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
5
Research Station of Vegetation Ecosystems on Coral Islands in South China Sea, Guangzhou 510650, China
*
Author to whom correspondence should be addressed.
Contributed equally to this work.
Academic Editor: Enriqueta Moyano Cañete
Genes 2021, 12(11), 1714; https://doi.org/10.3390/genes12111714
Received: 7 October 2021 / Revised: 20 October 2021 / Accepted: 23 October 2021 / Published: 27 October 2021
(This article belongs to the Section Plant Genetics and Genomics)
Red pitaya (Hylocereus polyrhizus) is a significant functional food that is largely planted in Southeast Asia. Heat stress (HS) induced by high temperatures is likely to restrict the growth and survival of red pitaya. Although pitaya can tolerate temperatures as high as 40 °C, little is known of how it can withstand HS. In this study, the transcriptomic and metabolomic responses of red pitaya seedlings to HS were analyzed. A total of 198 transcripts (122 upregulated and 76 downregulated) were significantly differentially expressed after 24 h and 72 h of exposure to 42 °C compared with a control grown at 28 °C. We also identified 64 differentially accumulated metabolites in pitaya under HS (37 increased and 27 decreased). These differential metabolites, especially amino acids, organic acids, and sugars, are involved in metabolic pathways and the biosynthesis of amino acids. Interaction network analysis of the heat-responsive genes and metabolites suggested that similar pathways and complex response mechanisms are involved in the response of pitaya to HS. Overexpression of one of the upregulated genes (contig10820) in Arabidopsis, which is a homolog of PR-1 and named HuPR-1, significantly increased tolerance to HS. This is the first study showing that HuPR-1 plays a role in the response of pitaya to abiotic stress. These findings provide valuable insights that will aid future studies examining adaptation to HS in pitaya. View Full-Text
Keywords: red pitaya; heat stress; transcriptome; metabolome; PR-1 protein red pitaya; heat stress; transcriptome; metabolome; PR-1 protein
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MDPI and ACS Style

Jiao, Z.; Xu, W.; Nong, Q.; Zhang, M.; Jian, S.; Lu, H.; Chen, J.; Zhang, M.; Xia, K. An Integrative Transcriptomic and Metabolomic Analysis of Red Pitaya (Hylocereus polyrhizus) Seedlings in Response to Heat Stress. Genes 2021, 12, 1714. https://doi.org/10.3390/genes12111714

AMA Style

Jiao Z, Xu W, Nong Q, Zhang M, Jian S, Lu H, Chen J, Zhang M, Xia K. An Integrative Transcriptomic and Metabolomic Analysis of Red Pitaya (Hylocereus polyrhizus) Seedlings in Response to Heat Stress. Genes. 2021; 12(11):1714. https://doi.org/10.3390/genes12111714

Chicago/Turabian Style

Jiao, Zhengli, Weijuan Xu, Quandong Nong, Mei Zhang, Shuguang Jian, Hongfang Lu, Jiantong Chen, Mingyong Zhang, and Kuaifei Xia. 2021. "An Integrative Transcriptomic and Metabolomic Analysis of Red Pitaya (Hylocereus polyrhizus) Seedlings in Response to Heat Stress" Genes 12, no. 11: 1714. https://doi.org/10.3390/genes12111714

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