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Open AccessArticle

Structure and Sequence of the Sex Determining Locus in Two Wild Populations of Nile Tilapia

1
UMR-Institut des Sciences de l’Evolution de Montpellier, Centre National de la Recherche Scientifique, Institut de Recherche pour le Développement, Ecole Pratique des Hautes Etudes, University of Montpellier, 34090 Montpellier, France
2
UMR-Institut des Sciences de l’Evolution de Montpellier, Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Campus Int. Baillarguet, 34398 Montpellier, France
3
Department of Biology, University of Maryland, College Park, MD 20742, USA
4
UMR BOREA, CNRS-7208/MNHN/UPMC/IRD-207/UCN/UA, Université des Antilles, 97159 Guadeloupe, France
5
Institute of Aquaculture, School of Natural Sciences, University of Stirling, Stirling, Scotland FK9 4LA, UK
*
Author to whom correspondence should be addressed.
Genes 2020, 11(9), 1017; https://doi.org/10.3390/genes11091017
Received: 17 July 2020 / Revised: 15 August 2020 / Accepted: 27 August 2020 / Published: 29 August 2020
In domesticated strains of the Nile tilapia, phenotypic sex has been linked to genetic variants on linkage groups 1, 20 and 23. This diversity of sex-loci might reflect a naturally polymorphic sex determination system in Nile tilapia, or it might be an artefact arising from the process of domestication. Here, we searched for sex-determiners in wild populations from Kpandu, Lake Volta (Ghana-West Africa), and from Lake Koka (Ethiopia-East Africa) that have not been subjected to any genetic manipulation. We analysed lab-reared families using double-digest Restriction Associated DNA sequencing (ddRAD) and analysed wild-caught males and females with pooled whole-genome sequencing (WGS). Strong sex-linked signals were found on LG23 in both populations, and sex-linked signals with LG3 were observed in Kpandu samples. WGS uncovered blocks of high sequence coverage, suggesting the presence of B chromosomes. We confirmed the existence of a tandem amh duplication in LG23 in both populations and determined its breakpoints between the oaz1 and dot1l genes. We found two common deletions of ~5 kb in males and confirmed the presence of both amhY and amh∆Y genes. Males from Lake Koka lack both the previously reported 234 bp deletion and the 5 bp frameshift-insertion that creates a premature stop codon in amh∆Y. View Full-Text
Keywords: sex-determinant; Y-chromosome; amh; male duplication; Nile tilapia sex-determinant; Y-chromosome; amh; male duplication; Nile tilapia
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Triay, C.; Conte, M.A.; Baroiller, J.-F.; Bezault, E.; Clark, F.E.; Penman, D.J.; Kocher, T.D.; D’Cotta, H. Structure and Sequence of the Sex Determining Locus in Two Wild Populations of Nile Tilapia. Genes 2020, 11, 1017.

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