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Open AccessArticle

Transcriptomic and Metabolomic Changes Triggered by Fusarium solani in Common Bean (Phaseolus vulgaris L.)

1
Integrated Plant Protection Center, Lishui Institute of Agricultural and Forestry Sciences, 827 Liyang Stress, Lishui 323000, China
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Plant Protection Station of Songyang County, Lishui 323400, China
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Weihai Academy of Agricultural Sciences, No. 411, Tongyi Road, Weihai 311300, China
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School of Agricultural and Food Science, Zhejiang A&F University, Hangzhou 311300, China
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College of Ecology, Lishui University, Lishui, Zhejiang 323000, China
*
Author to whom correspondence should be addressed.
Genes 2020, 11(2), 177; https://doi.org/10.3390/genes11020177
Received: 31 December 2019 / Revised: 25 January 2020 / Accepted: 3 February 2020 / Published: 7 February 2020
(This article belongs to the Special Issue Genetic Improvement of Cereals and Grain Legumes)
Common bean (Phaseolus vulgaris L.) is a major legume and is frequently attacked by fungal pathogens, including Fusarium solani f. sp. phaseoli (FSP), which cause Fusarium root rot. FSP substantially reduces common bean yields across the world, including China, but little is known about how common bean plants defend themselves against this fungal pathogen. In the current study, we combined next-generation RNA sequencing and metabolomics techniques to investigate the changes in gene expression and metabolomic processes in common bean infected with FSP. There were 29,722 differentially regulated genes and 300 differentially regulated metabolites between control and infected plants. The combined omics approach revealed that FSP is perceived by PAMP-triggered immunity and effector-triggered immunity. Infected seedlings showed that common bean responded by cell wall modification, ROS generation, and a synergistic hormone-driven defense response. Further analysis showed that FSP induced energy metabolism, nitrogen mobilization, accumulation of sugars, and arginine and proline metabolism. Importantly, metabolic pathways were most significantly enriched, which resulted in increased levels of metabolites that were involved in the plant defense response. A correspondence between the transcript pattern and metabolite profile was observed in the discussed pathways. The combined omics approach enhances our understanding of the less explored pathosystem and will provide clues for the development of common bean cultivars’ resistant to FSP. View Full-Text
Keywords: common bean; Fusarium sp; fungus–plant interactions; induced response; transcriptome; metabolome common bean; Fusarium sp; fungus–plant interactions; induced response; transcriptome; metabolome
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Chen, L.; Wu, Q.; He, T.; Lan, J.; Ding, L.; Liu, T.; Wu, Q.; Pan, Y.; Chen, T. Transcriptomic and Metabolomic Changes Triggered by Fusarium solani in Common Bean (Phaseolus vulgaris L.). Genes 2020, 11, 177.

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