Next Article in Journal
Eco-Evolutionary Feedbacks and the Maintenance of Metacommunity Diversity in a Changing Environment
Previous Article in Journal
PupStruct: Prediction of Pupylated Lysine Residues Using Structural Properties of Amino Acids
Previous Article in Special Issue
Slow Adaptive Response of Budding Yeast Cells to Stable Conditions of Continuous Culture Can Occur without Genome Modifications
Article

Eukaryotic Elongation Factor 3 Protects Saccharomyces cerevisiae Yeast from Oxidative Stress

Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Genes 2020, 11(12), 1432; https://doi.org/10.3390/genes11121432
Received: 26 October 2020 / Revised: 26 November 2020 / Accepted: 27 November 2020 / Published: 28 November 2020
(This article belongs to the Special Issue Genetic Aspects of Yeast: Cell Biology, Ecology and Biotechnology)
Translation is a core process of cellular protein homeostasis and, thus, needs to be tightly regulated. The production of newly synthesized proteins adapts to the current needs of the cell, including the response to conditions of oxidative stress. Overall protein synthesis decreases upon oxidative stress. However, the selective production of proteins is initiated to help neutralize stress conditions. In contrast to higher eukaryotes, fungi require three translation elongation factors, eEF1, eEF2, and eEF3, for protein synthesis. eEF1 and eEF2 are evolutionarily conserved, but they alone are insufficient for the translation elongation process. eEF3 is encoded by two paralogous genes, YEF3 and HEF3. However, only YEF3 is essential in yeast, whereas the function of HEF3 remains unknown. To elucidate the cellular function of Hef3p, we used cells that were depleted of HEF3 and treated with H2O2 and analyzed the growth of yeast, global protein production, and protein levels. We found that HEF3 is necessary to withstand oxidative stress conditions, suggesting that Hef3p is involved in the selective production of proteins that are necessary for defense against reactive oxygen species. View Full-Text
Keywords: eEF3; HEF3; YEF3; oxidative stress; reactive oxygen species (ROS); translation; elongation; fungi eEF3; HEF3; YEF3; oxidative stress; reactive oxygen species (ROS); translation; elongation; fungi
Show Figures

Figure 1

MDPI and ACS Style

Gościńska, K.; Shahmoradi Ghahe, S.; Domogała, S.; Topf, U. Eukaryotic Elongation Factor 3 Protects Saccharomyces cerevisiae Yeast from Oxidative Stress. Genes 2020, 11, 1432. https://doi.org/10.3390/genes11121432

AMA Style

Gościńska K, Shahmoradi Ghahe S, Domogała S, Topf U. Eukaryotic Elongation Factor 3 Protects Saccharomyces cerevisiae Yeast from Oxidative Stress. Genes. 2020; 11(12):1432. https://doi.org/10.3390/genes11121432

Chicago/Turabian Style

Gościńska, Karolina, Somayeh Shahmoradi Ghahe, Sara Domogała, and Ulrike Topf. 2020. "Eukaryotic Elongation Factor 3 Protects Saccharomyces cerevisiae Yeast from Oxidative Stress" Genes 11, no. 12: 1432. https://doi.org/10.3390/genes11121432

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop