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Genes 2019, 10(3), 196; https://doi.org/10.3390/genes10030196

Cohesin-Mediated Genome Architecture Does Not Define DNA Replication Timing Domains

Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
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Received: 14 January 2019 / Revised: 7 February 2019 / Accepted: 19 February 2019 / Published: 4 March 2019
(This article belongs to the Special Issue DNA Replication Timing: Where, When, How and Why?)
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Abstract

3D genome organization is strongly predictive of DNA replication timing in mammalian cells. This work tested the extent to which loop-based genome architecture acts as a regulatory unit of replication timing by using an auxin-inducible system for acute cohesin ablation. Cohesin ablation in a population of cells in asynchronous culture was shown not to disrupt patterns of replication timing as assayed by replication sequencing (RepliSeq) or BrdU-focus microscopy. Furthermore, cohesin ablation prior to S phase entry in synchronized cells was similarly shown to not impact replication timing patterns. These results suggest that cohesin-mediated genome architecture is not required for the execution of replication timing patterns in S phase, nor for the establishment of replication timing domains in G1. View Full-Text
Keywords: replication timing; cohesin; genome architecture; chromatin interactions; DNA replication timing; cohesin; genome architecture; chromatin interactions; DNA
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Oldach, P.; Nieduszynski, C.A. Cohesin-Mediated Genome Architecture Does Not Define DNA Replication Timing Domains. Genes 2019, 10, 196.

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