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Genes 2019, 10(3), 196;

Cohesin-Mediated Genome Architecture Does Not Define DNA Replication Timing Domains

Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
Author to whom correspondence should be addressed.
Received: 14 January 2019 / Revised: 7 February 2019 / Accepted: 19 February 2019 / Published: 4 March 2019
(This article belongs to the Special Issue DNA Replication Timing: Where, When, How and Why?)
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3D genome organization is strongly predictive of DNA replication timing in mammalian cells. This work tested the extent to which loop-based genome architecture acts as a regulatory unit of replication timing by using an auxin-inducible system for acute cohesin ablation. Cohesin ablation in a population of cells in asynchronous culture was shown not to disrupt patterns of replication timing as assayed by replication sequencing (RepliSeq) or BrdU-focus microscopy. Furthermore, cohesin ablation prior to S phase entry in synchronized cells was similarly shown to not impact replication timing patterns. These results suggest that cohesin-mediated genome architecture is not required for the execution of replication timing patterns in S phase, nor for the establishment of replication timing domains in G1. View Full-Text
Keywords: replication timing; cohesin; genome architecture; chromatin interactions; DNA replication timing; cohesin; genome architecture; chromatin interactions; DNA

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Oldach, P.; Nieduszynski, C.A. Cohesin-Mediated Genome Architecture Does Not Define DNA Replication Timing Domains. Genes 2019, 10, 196.

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