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RPAP3 C-Terminal Domain: A Conserved Domain for the Assembly of R2TP Co-Chaperone Complexes

Structural Biology Programme, Spanish National Cancer Research Centre (CNIO), Melchor Fernández Almagro 3, 28029 Madrid, Spain
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Author to whom correspondence should be addressed.
Cells 2020, 9(5), 1139; https://doi.org/10.3390/cells9051139
Received: 27 March 2020 / Revised: 28 April 2020 / Accepted: 2 May 2020 / Published: 6 May 2020
(This article belongs to the Special Issue Molecular Chaperones: Cancer and Cell Death)
The Rvb1-Rvb2-Tah1-Pih1 (R2TP) complex is a co-chaperone complex that works together with HSP90 in the activation and assembly of several macromolecular complexes, including RNA polymerase II (Pol II) and complexes of the phosphatidylinositol-3-kinase-like family of kinases (PIKKs), such as mTORC1 and ATR/ATRIP. R2TP is made of four subunits: RuvB-like protein 1 (RUVBL1) and RuvB-like 2 (RUVBL2) AAA-type ATPases, RNA polymerase II-associated protein 3 (RPAP3), and the Protein interacting with Hsp90 1 (PIH1) domain-containing protein 1 (PIH1D1). R2TP associates with other proteins as part of a complex co-chaperone machinery involved in the assembly and maturation of a growing list of macromolecular complexes. Recent progress in the structural characterization of R2TP has revealed an alpha-helical domain at the C-terminus of RPAP3 that is essential to bring the RUVBL1 and RUVBL2 ATPases to R2TP. The RPAP3 C-terminal domain interacts directly with RUVBL2 and it is also known as RUVBL2-binding domain (RBD). Several human proteins contain a region homologous to the RPAP3 C-terminal domain, and some are capable of assembling R2TP-like complexes, which could have specialized functions. Only the RUVBL1-RUVBL2 ATPase complex and a protein containing an RPAP3 C-terminal-like domain are found in all R2TP and R2TP-like complexes. Therefore, the RPAP3 C-terminal domain is one of few components essential for the formation of all R2TP and R2TP-like co-chaperone complexes. View Full-Text
Keywords: RUVBL1; RUVBL2; RPAP3; PIH1D1; R2TP; HSP90 co-chaperone; TPR domain; URI1 prefoldin complex RUVBL1; RUVBL2; RPAP3; PIH1D1; R2TP; HSP90 co-chaperone; TPR domain; URI1 prefoldin complex
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MDPI and ACS Style

Rodríguez, C.F.; Llorca, O. RPAP3 C-Terminal Domain: A Conserved Domain for the Assembly of R2TP Co-Chaperone Complexes. Cells 2020, 9, 1139. https://doi.org/10.3390/cells9051139

AMA Style

Rodríguez CF, Llorca O. RPAP3 C-Terminal Domain: A Conserved Domain for the Assembly of R2TP Co-Chaperone Complexes. Cells. 2020; 9(5):1139. https://doi.org/10.3390/cells9051139

Chicago/Turabian Style

Rodríguez, Carlos F., and Oscar Llorca. 2020. "RPAP3 C-Terminal Domain: A Conserved Domain for the Assembly of R2TP Co-Chaperone Complexes" Cells 9, no. 5: 1139. https://doi.org/10.3390/cells9051139

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